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Bacteria: Third RNA binding protein identified

Date:
October 17, 2016
Source:
Julius-Maximilians-Universität Würzburg, JMU
Summary:
Pathogenic bacteria use small RNA molecules to adapt to their environment. Infection researchers have now pinpointed a protein involved in regulating the activity of these molecules.
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The bacterial RNA universe: The structures of the different regulatory RNA molecules are shown left, their preferred protein binding partners on the right.
Credit: Alexandre Smirnov

Pathogenic bacteria use small RNA molecules to adapt to their environment. Infection researchers from Würzburg have now pinpointed a protein involved in regulating the activity of these molecules.

Small regulatory RNA molecules are vital for salmonella and other bacteria potentially harmful to humans: This RNA type controls gene activity and allows bacteria to quickly adjust to changing conditions of living and stress as are typical during an infection, for example, when entering the blood stream or inside human cells.

Professor Jörg Vogel, head of the Institute for Molecular Infection Biology of the Julius-Maximilians-Universität Würzburg (JMU) in Bavaria, Germany, is a pioneer in researching small regulatory RNA molecules. He and his team are determined to get to the bottom of how these molecules work and act. His works could also show new ways to fight pathogens.

ProQ binds nearly 100 regulatory RNAs

New findings from Vogel's team have now been published in the journal PNAS: So far, two proteins (Hfg and CsrA) have been known to bind closely to the bacteria's regulatory RNA molecules and influence their activities. Using a new self-designed method, the Würzburg team has now discovered a long-suspected third protein (ProQ) whose function inside the cell has been unknown until recently.

Experiments showed that the ProQ protein binds to 98 regulatory RNAs of the enterobacterial Salmonella enterica. The bacterium has been found to have around 300 such RNAs in total. Moreover, ProQ seems to have specialised in RNA molecules with a rather complex structure.

This protein and the RNA molecules that bind to it represent a largely unresearched class of gene activity regulators in the bacterial "RNA universe." "It will be particularly exciting to find out how ProQ is able to pinpoint the highly structured RNAs among millions of other RNA molecules in a cell," says Jörg Vogel.

PNAS considers results significant

These results have been reported in PNAS by the Würzburg professor together with Alexandre Smirnov, Konrad Förstner, Erik Holmqvist and Regina Günster as well as colleagues from Greifswald and Cologne. Considered highly significant for bacterial research, the new findings are featured in the journal's "Research Highlight" section.

A new technique developed by the JMU team has successfully tracked down the activities of the ProQ protein. "The methods available so far were subject to certain limits with regard to detecting and generally classifying RNA protein interactions which we have overcome here," Professor Vogel further. Since the new method can basically be applied to any other organism, it is expected to provide more progress in researching regulatory RNA.


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Materials provided by Julius-Maximilians-Universität Würzburg, JMU. Note: Content may be edited for style and length.


Journal Reference:

  1. Alexandre Smirnov, Konrad U. Förstner, Erik Holmqvist, Andreas Otto, Regina Günster, Dörte Becher, Richard Reinhardt, Jörg Vogel. Grad-seq guides the discovery of ProQ as a major small RNA-binding protein. Proceedings of the National Academy of Sciences, 2016; 113 (41): 11591 DOI: 10.1073/pnas.1609981113

Cite This Page:

Julius-Maximilians-Universität Würzburg, JMU. "Bacteria: Third RNA binding protein identified." ScienceDaily. ScienceDaily, 17 October 2016. <www.sciencedaily.com/releases/2016/10/161017123837.htm>.
Julius-Maximilians-Universität Würzburg, JMU. (2016, October 17). Bacteria: Third RNA binding protein identified. ScienceDaily. Retrieved May 25, 2017 from www.sciencedaily.com/releases/2016/10/161017123837.htm
Julius-Maximilians-Universität Würzburg, JMU. "Bacteria: Third RNA binding protein identified." ScienceDaily. www.sciencedaily.com/releases/2016/10/161017123837.htm (accessed May 25, 2017).

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