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When protein crystals grow

Date:
January 4, 2017
Source:
University of Vienna
Summary:
Scientists are investigating so-called polyoxometalates. These compounds exhibit a great diversity and offer the scientists a wide range of applications. In interaction with enzymes they can enable the crystallization of proteins. On the other hand, the polyoxometalates represent compounds with an enormous application potential in catalysis and materials science.
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Showcasing research from Annette Rompel's Laboratory at the Department of Biophysical Chemistry, University of Vienna, Austria.
Credit: Annette Rompel

Polyoxometalates (POM) are a substance class consisting of anionic metal oxide clusters, which have a great structural diversity and outstanding properties and therefore offer a wide range of possible applications. One of the most prominent POM structures is the so-called Anderson-Evans structure, which is studied intensively by Rompel's working group. The tellurium-centered Anderson polyoxotungstate (TEW) has been applied by Rompel for the first time as a successful additive in protein crystallization.

"In order to understand the interaction between a protein and TEW, we tried to obtain protein crystals in the presence of TEW and also without the TEW," explains Rompel. This has been successful with the enzyme Aurone synthase. A novel structure was obtained, which for the first time contains a bond between the TEW and the protein. The crystallization additive was flexible and able to adapt to the conditions in the protein, which makes TEW particularly valuable as an additive in protein crystallization. By now, Annette Rompel has succeeded in crystallizing three different proteins using TEW.

In addition, the chemist now tries to link functional groups to the Anderson basic structure -- with the aim of producing new materials. "After the functionalization of the first Anderson polyoxotungstate by our group, it will now be possible to produce novel functional materials," says Rompel.

In addition to chemical applications, Rompel's findings are highly relevant for biological, biochemical, medical and pharmaceutical studies. Protein crystallography is currently the most productive and widely used method to obtain structural information about proteins. The structural findings are important for understanding the protein function, but also for the development of protein-ligand molecules, which can be small organic substances and also larger molecules such as antibodies, influencing the protein function. "Our research shows the importance of TEW as an additive in protein crystallization. Our proposed synthesis strategy opens the door to new multifunctional organic-inorganic hybrid materials," concludes Annette Rompel.


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Journal References:

  1. Christian Molitor, Aleksandar Bijelic, Annette Rompel. In situ formation of the first proteinogenically functionalized [TeW6O24O2(Glu)]7−structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun., 2016; 52 (83): 12286 DOI: 10.1039/C6CC07004C
  2. Christian Molitor, Aleksandar Bijelic, Annette Rompel. In situ formation of the first proteinogenically functionalized [TeW6O24O2(Glu)]7−structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun., 2016; 52 (83): 12286 DOI: 10.1039/C6CC07004C
  3. Nadiia I. Gumerova, Alexander Roller, Annette Rompel. [Ni(OH)3W6O18(OCH2)3CCH2OH]4−: the first tris-functionalized Anderson-type heteropolytungstate. Chem. Commun., 2016; 52 (59): 9263 DOI: 10.1039/C6CC04326G
  4. Amir Blazevic, Annette Rompel. The Anderson–Evans polyoxometalate: From inorganic building blocks via hybrid organic–inorganic structures to tomorrows “Bio-POM”. Coordination Chemistry Reviews, 2016; 307: 42 DOI: 10.1016/j.ccr.2015.07.001

Cite This Page:

University of Vienna. "When protein crystals grow." ScienceDaily. ScienceDaily, 4 January 2017. <www.sciencedaily.com/releases/2017/01/170104104308.htm>.
University of Vienna. (2017, January 4). When protein crystals grow. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2017/01/170104104308.htm
University of Vienna. "When protein crystals grow." ScienceDaily. www.sciencedaily.com/releases/2017/01/170104104308.htm (accessed May 26, 2017).

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