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Staying in shape: How rod-shaped bacteria grow long, not wide

Date:
May 20, 2019
Source:
Marine Biological Laboratory
Summary:
Researchers show how the rod-shaped bacteria Bacillus subtilis maintains its precise diameter while growing end to end.
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The slender, rod-shaped Bacillus subtilis is one of the best-studied bacteria in the world, a go-to system for exploring and understanding how bacteria grow, replicate, and divide. One of its outstanding mysteries has been how it manages to keep its precise diameter while growing and and getting bigger end-to-end.

This week, a team led by Ethan Garner of Harvard University describes the opposing and balanced enzymatic actions that keep B. subtilis from bulging wide while it builds up its inner cell wall and elongates. The study, in Nature Microbiology, is a collaboration with microscopy developer Rudolf Oldenbourg of the Marine Biological Laboratory (MBL).

"I had been impressed by Rudolf's work for many years and always hoped that I (or someone) would introduce polarization microscopy to bacterial cell biology," Garner says. This paper was his opportunity.

With polarization microscopy, scientists can visualize the orientation of individual molecules in a live cell, and how that orientation may change over time. "Polarization microscopy was key to this project," Garner says, giving his team essential and hard-to-obtain information on the orientation of material that B. subtilis adds to its cell wall as it grows.

"As I have been giving talks on this work, the bacterial community has been incredibly impressed by this [polarization microscopy] assay," Garner says. "There are many other bacteria that people want to explore with it."

Oldenbourg, a senior scientist at MBL, is happy to oblige. "We are standing ready to support the bacteria research community through the OpenPolScope Resource at MBL," he says.


Story Source:

Materials provided by Marine Biological Laboratory. Original written by Diana Kenney. Note: Content may be edited for style and length.


Journal Reference:

  1. Michael F. Dion, Mrinal Kapoor, Yingjie Sun, Sean Wilson, Joel Ryan, Antoine Vigouroux, Sven van Teeffelen, Rudolf Oldenbourg, Ethan C. Garner. Bacillus subtilis cell diameter is determined by the opposing actions of two distinct cell wall synthetic systems. Nature Microbiology, 2019; DOI: 10.1038/s41564-019-0439-0

Cite This Page:

Marine Biological Laboratory. "Staying in shape: How rod-shaped bacteria grow long, not wide." ScienceDaily. ScienceDaily, 20 May 2019. <www.sciencedaily.com/releases/2019/05/190520165015.htm>.
Marine Biological Laboratory. (2019, May 20). Staying in shape: How rod-shaped bacteria grow long, not wide. ScienceDaily. Retrieved June 24, 2024 from www.sciencedaily.com/releases/2019/05/190520165015.htm
Marine Biological Laboratory. "Staying in shape: How rod-shaped bacteria grow long, not wide." ScienceDaily. www.sciencedaily.com/releases/2019/05/190520165015.htm (accessed June 24, 2024).

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