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Empty spaces, how do they make a protein unstable?

Date:
October 15, 2019
Source:
Aarhus University
Summary:
Partial unfolding of proteins can be a major challenge in the industry, as it may affect the stability of products. So how does an empty space or cavity in its hydrophobic core destabilize a protein? And would such a cavity, in fact, be empty? These are some of the questions that researchers answer in a new study.
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Proteins exist as groups of microscopic configurations, regulated by a landscape of free energy, in which there is a multitude of "excited" states that co-exists with the minimum energy structure. These alternatively folded and partially "disordered" states occur continuously due to protein dynamics and are key elements required to understand the function and stability of proteins.

Because these excited states exist only briefly and are lowly populated they are "invisible" to most experimental methods. However, recent developments in NMR spectroscopy allow for their detection and structural investigation at atomic resolution.

In this study, the researchers used a classic model system for protein folding, the L99A mutant of T4 lysozyme. This protein has a cavity in its hydrophobic core that is large enough to fit a benzene ring (a chemical compound consisting of a ring of 6 carbon atoms).

Pressure reveals invisible states

Mulder and his team used Nuclear Magnetic Resonance (NMR) spectroscopy coupled with hydrostatic pressure to monitor "invisible" excited states. High pressure favors compact states, and the protein unfolds or collapses at high pressure to remove cavities.

The researchers have succeeded in obtaining a unique picture of the hierarchy of unfolded states in the protein's energy landscape by subjecting it to pressure. Furthermore, with these pressure perturbations, they have been able to identify empty protein cavities and determine the energetic consequences of filling these with water.

Partial unfolding of unstable parts of proteins is a major concern in the development of industrial enzymes and biological drugs, as well as a starting point for protein deposition diseases. The approach shown in this study here establishes a powerful way to rationally understand and gain control of protein stability at the atomic level.


Story Source:

Materials provided by Aarhus University. Note: Content may be edited for style and length.


Journal Reference:

  1. Mengjun Xue, Takuro Wakamoto, Camilla Kejlberg, Yuichi Yoshimura, Tania Aaquist Nielsen, Michael Wulff Risør, Kristian Wejse Sanggaard, Ryo Kitahara, Frans A. A. Mulder. How internal cavities destabilize a protein. Proceedings of the National Academy of Sciences, 2019; 201911181 DOI: 10.1073/pnas.1911181116

Cite This Page:

Aarhus University. "Empty spaces, how do they make a protein unstable?." ScienceDaily. ScienceDaily, 15 October 2019. <www.sciencedaily.com/releases/2019/10/191015113311.htm>.
Aarhus University. (2019, October 15). Empty spaces, how do they make a protein unstable?. ScienceDaily. Retrieved April 17, 2024 from www.sciencedaily.com/releases/2019/10/191015113311.htm
Aarhus University. "Empty spaces, how do they make a protein unstable?." ScienceDaily. www.sciencedaily.com/releases/2019/10/191015113311.htm (accessed April 17, 2024).

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