New! Sign up for our free email newsletter.
Science News
from research organizations

New imaging method reveals causes of cerebral oedema

Date:
January 31, 2022
Source:
Heinrich-Heine University Duesseldorf
Summary:
Cerebral oedema is a dangerous complication in many brain-related conditions such as strokes. Researchers have now developed a new measurement method that enables a better understanding of the cellular causes of cerebral oedema. According to a new study, the TRPV4 ion channel in particular plays an important role.
Share:
FULL STORY

Cerebral oedema is a dangerous complication in many brain-related conditions such as strokes. Researchers at the Institute of Neurobiology at Heinrich Heine University Düsseldorf (HHU) have developed a new measurement method in collaboration with colleagues from Bonn and with the involvement of a Berlin-based optoelectronics company that enables better understanding of the cellular causes of cerebral oedema. In the latest issue of the Journal of the American Society for Neuroscience they describe how the TRPV4 ion channel in particular plays an important role.

Our brain is well-protected by the bone of the skull. However, many illnesses lead to swelling of the cerebral tissue, which is referred to as "cerebral oedema." As the brain cannot expand within the skull, this swelling often results in a dangerous rise in intercranial pressure. This in turn damages further brain cells and may, for example in the case of causative strokes, further impair blood supply to the brain.

There are many causes of cerebral oedema, yet even today there are few therapeutic approaches for treating them successfully. Consequently, many patients require an operation to remove part of the skull bone -- a so-called craniotomy -- to ensure sufficient space for the brain. However, this operation is not without risks -- and it does not eliminate the dangerous swelling.

In collaboration with the company Picoquant, Professor Dr. Christine Rose and her team from the Institute of Neurobiology at HHU have now developed a new method with which they can depict the changes that lead to the swelling of nerve cells in real time. This imaging method, known as "rapidFLIM" (Fluorescence Lifetime IMaging), permits the depiction of cellular processes at an unprecedented temporal resolution. Professor Dr. Christian Henneberger from the University of Bonn provided further conceptual support.

In the paper they have now published, the researchers recreated the conditions to which nerve cells are exposed during an ischaemic stroke in the laboratory. Dr. Jan Meyer, one of the two lead authors of the study, says: "Using rapidFLIM we can show that a breakdown in cellular energy supply -- one of the principal side effects of a stroke -- results in nerve cells quickly becoming charged with sodium ions. This in turn is a key cause of the subsequent swelling of cells."

Dr. Niklas Gerkau, co-lead author, adds: "Previous methods were unable to depict properly how this sodium charging develops over time and its extent. Combining rapidFLIM with our high-resolution, multi-photon microscopy opens up new perspectives for us and allows a better understanding of the sodium regulation of nerve cells."

In their study, the researchers also discovered a previously unknown mechanism for this fatal sodium charging in which the TRPV4 ion channel in the nerve cells plays a key role. This channel is instrumental in determining how much of the element sodium enters the cell. Professor Rose comments: "The TRPV4 channel is a promising starting point for limiting cellular damage and infarction size after an ischaemic stroke."

The research work was conducted within the framework of the research group FOR 2795 "Synapses under stress: Early events induced by metabolic failure at glutamatergic synapses" coordinated by Professor Rose at HHU. Professor Henneberger is also part of this group. The Ilselore Luckow Foundation also supported the work.


Story Source:

Materials provided by Heinrich-Heine University Duesseldorf. Original written by Arne Claussen. Note: Content may be edited for style and length.


Journal Reference:

  1. Jan Meyer, Niklas J. Gerkau, Karl W. Kafitz, Matthias Patting, Fabian Jolmes, Christian Henneberger, Christine R. Rose. Rapid Fluorescence Lifetime Imaging Reveals That TRPV4 Channels Promote Dysregulation of Neuronal Na in Ischemia. The Journal of Neuroscience, 2022; 42 (4): 552 DOI: 10.1523/JNEUROSCI.0819-21.2021

Cite This Page:

Heinrich-Heine University Duesseldorf. "New imaging method reveals causes of cerebral oedema." ScienceDaily. ScienceDaily, 31 January 2022. <www.sciencedaily.com/releases/2022/01/220131110502.htm>.
Heinrich-Heine University Duesseldorf. (2022, January 31). New imaging method reveals causes of cerebral oedema. ScienceDaily. Retrieved May 23, 2024 from www.sciencedaily.com/releases/2022/01/220131110502.htm
Heinrich-Heine University Duesseldorf. "New imaging method reveals causes of cerebral oedema." ScienceDaily. www.sciencedaily.com/releases/2022/01/220131110502.htm (accessed May 23, 2024).

Explore More

from ScienceDaily

RELATED STORIES