Soft devices--powered by 'stressed' algae -- glow in the dark when squished or stretched

The work was published recently in Nature Communications.

The researchers took their inspiration for these devices from the bioluminescent waves that sometimes occur at San Diego's beaches during red tide events. Shengqiang Cai, a professor of mechanical and aerospace engineering at the UC San Diego Jacobs School of Engineering and the study's senior author, was watching the glowing blue waves with his family one spring night and was curious to learn more about what causes this impressive display.

The source of the glow is a type of single-celled algae called dinoflagellates. But what fascinated Cai in particular was learning that dinoflagellates produce light when subjected to mechanical stress, such as the forces from the ocean waves. "This was very interesting to me because my research focuses on the mechanics of materials -- anything related to how deformation and stress affect material behavior," he said.

Cai wanted to harness this natural glow to develop devices for soft robots that can be used in the dark without electricity. He teamed up with Michael Latz, a marine biologist at UC San Diego's Scripps Institution of Oceanography, who studies bioluminescence in dinoflagellates and how it responds to various water flow conditions. The collaboration was a perfect opportunity to merge Latz's fundamental research on bioluminescence with Cai's materials science work for robotics applications.

To make the devices, the researchers inject a culture solution of the dinoflagellate Pyrocystis lunula inside a cavity of a soft, stretchy, transparent material. The material can be any shape -- here, the researchers tested a variety of shapes including flat sheets, X-shaped structures and small pouches.

When the material is pressed, stretched, or deformed in any way, it causes the dinoflagellate solution inside to flow. The mechanical stress from that flow triggers the dinoflagellates to glow. A key feature of the design here is that the inner surface of the material is lined with small pillars to give it a rough inner texture. This disturbs the fluid flow inside the material and makes it stronger. A stronger flow applies more stress to the dinoflagellates, which in turn triggers a brighter glow.

The devices are so sensitive that even a soft tap is enough to make them glow. The researchers also made the devices glow by vibrating them, drawing on their surfaces, and blowing air on them to make them bend and sway -- which shows that they could potentially be used to harvest air flow to produce light. The researchers also inserted small magnets inside the devices so that they can be magnetically steered, glowing as they move and contort.

The devices can be recharged with light. The dinoflagellates are photosynthetic, meaning they use sunlight to produce food and energy. Shining light on the devices during the day gives them the juice they need to glow during the night.

The beauty of these devices, noted Cai, is their simplicity. "They are basically maintenance-free. Once we inject culture solution into the materials, that's it. As long as they get recharged with sunlight, they can be used over and over again for at least a month. We don't need to change out the solution or anything. Each device is its own little ecosystem -- an engineered living material."

The biggest challenge was figuring out how to keep the dinoflagellates alive and thriving inside the material structures. "When you're putting living organisms inside a synthetic, enclosed space, you need to think about how to make that space habitable -- how it will let air in and out, for example -- while still keeping the material properties that you want," said study first author Chenghai Li, a mechanical and aerospace engineering Ph.D. student in Cai's lab. The key, noted Li, was to make the elastic polymer that he worked with porous enough for gases like oxygen to pass through without having the culture solution leak out. The dinoflagellates can survive for more than a month inside this material.

The researchers are now creating new glowing materials with the dinoflagellates. In this study, the dinoflagellates simply fill the cavity of an already existing material. In the next stage of their work, the team is using them as an ingredient of the material itself. "This could provide more versatility in the sizes and shapes that we can experiment with moving forward," said Li.

The team is excited about the possibilities this work could bring to the fields of marine biology and materials science. "This is a neat demonstration of using living organisms for an engineering application," said Latz. "This work continues to advance our understanding of bioluminescent systems from the basic research side while setting the stage for a variety of applications, ranging from biological force sensors to electronics-free robotics and much more."

This work was supported by the Office of Naval Research (grant N00014-17-1-2062) and the U.S. Army Research Office (grant 2911NF-20-2-0182).

Video: https://youtu.be/JhwUB7KIex4