Featured Research

from universities, journals, and other organizations

New, Quicker Tests Identify E. Coli Strains

Date:
January 1, 2004
Source:
USDA / Agricultural Research Service
Summary:
New tests that more quickly identify dangerous strains of Escherichia coli bacteria are being developed by Agricultural Research Service scientists in Wyndmoor, Pa.

New tests that more quickly identify dangerous strains of Escherichia coli bacteria are being developed by Agricultural Research Service scientists in Wyndmoor, Pa.

ARS microbiologist Pina M. Fratamico, at the agency's Eastern Regional Research Center (ERRC) in Wyndmoor, is working with Pennsylvania State University to develop tests that quickly identify E. coli strains.

Certain E. coli strains, such as O157:H7, causes serious diseases, including bloody diarrhea and hemorrhagic colitis. Infections may result in serious health complications, including kidney failure. Other E. coli serogroups, including E. coli O26, O111 and O121, also cause gastrointestinal illnesses in humans.

Currently, scientists commonly use a procedure called serotyping to distinguish between different types of E. coli--some harmful, others harmless. However, this procedure is time-consuming and labor-intensive.

Fratamico, with ERRC's Microbial Food Safety Research Unit, and her team are developing both conventional and real-time polymerase chain reaction (PCR) tests. These chemical procedures generate enough of a bacterium's genetic material so that it can be studied and identified. With one real-time PCR reaction, four products can be amplified simultaneously and detected in "real time" as they multiply.

Scientists have little information about some individual E. coli serogroups; therefore, the number of diseases these organisms cause is likely underestimated. Fratamico is targeting genes in the E. coli O-antigen gene clusters so researchers can detect and identify specific serogroups and increase knowledge about each one's potency.

In one study, a real-time PCR assay was more sensitive than other detection methods. According to Fratamico, the U.S. Department of Agriculture's Food Safety and Inspection Service has expressed interest in the new PCR tests for detection and confirmation of not only E. coli O157:H7, but of other E. coli strains as well.

ARS is the USDA's chief scientific research agency.


Story Source:

The above story is based on materials provided by USDA / Agricultural Research Service. Note: Materials may be edited for content and length.


Cite This Page:

USDA / Agricultural Research Service. "New, Quicker Tests Identify E. Coli Strains." ScienceDaily. ScienceDaily, 1 January 2004. <www.sciencedaily.com/releases/2004/01/040101093850.htm>.
USDA / Agricultural Research Service. (2004, January 1). New, Quicker Tests Identify E. Coli Strains. ScienceDaily. Retrieved April 20, 2014 from www.sciencedaily.com/releases/2004/01/040101093850.htm
USDA / Agricultural Research Service. "New, Quicker Tests Identify E. Coli Strains." ScienceDaily. www.sciencedaily.com/releases/2004/01/040101093850.htm (accessed April 20, 2014).

Share This



More Plants & Animals News

Sunday, April 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Vermont Goat Meat Gives Refugees Taste of Home

Vermont Goat Meat Gives Refugees Taste of Home

AP (Apr. 18, 2014) Dairy farmers and ethnic groups in Vermont are both benefiting from a unique collaborative effort that's feeding a growing need for fresh and affordable goat meat. (April 18) Video provided by AP
Powered by NewsLook.com
Man Claims He Found Loch Ness Monster With... Apple Maps?

Man Claims He Found Loch Ness Monster With... Apple Maps?

Newsy (Apr. 18, 2014) Andy Dixon showed the Daily Mail a screenshot of what he believes to be the mythical beast swimming just below the lake's surface. Video provided by Newsy
Powered by NewsLook.com
First Ever 'Female Penis' Discovered In Animal Kingdom

First Ever 'Female Penis' Discovered In Animal Kingdom

Newsy (Apr. 18, 2014) Not only are these newly discovered bugs' sex organs reversed, but they also mate for up to 70 hours. Video provided by Newsy
Powered by NewsLook.com
Little Progress Made In Fighting Food Poisoning, CDC Says

Little Progress Made In Fighting Food Poisoning, CDC Says

Newsy (Apr. 18, 2014) A new report shows rates of two foodborne infections increased in the U.S. in recent years, while salmonella actually dropped 9 percent. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins