Featured Research

from universities, journals, and other organizations

Research Accelerates Discovery Of Novel Gene Function

Date:
July 9, 2004
Source:
University Of North Carolina School Of Medicine
Summary:
University of North Carolina at Chapel Hill researchers have developed a new technique for rapidly identifying the functions of genes.

CHAPEL HILL -- University of North Carolina at Chapel Hill researchers have developed a new technique for rapidly identifying the functions of genes.

The "high throughput" technique can be used in both cell culture and in animal models to screen thousands of genes for a particular biological function. It provides a method for the rapid development of a cDNA library, which would contain protein-encoding sequences of DNA. Researchers then can use the library to analyze a specific gene function.

The report appears in the July issue of Molecular Therapy, the American Society of Gene Therapy's journal.

"All the genes in the human genome have now been sequenced, but the problem is that we don't know their function," said Dr. Tal Kafri, principal investigator of the study and an assistant professor of microbiology and immunology at UNC's School of Medicine. He also is a member of the UNC Gene Therapy Center.

The study helps to resolve two bottlenecks in determining gene functions, he said.

"It offers a quick and efficient way to transfer cDNA into a viral vector library, and it also helps isolate altered cells, ensuring that the changes in them are due to the introduced gene. The closed system we have developed allows us to take candidate genes from virus to bacteria to cell to animal, quickly and efficiently."

The technique system may have clinical applications, including drug design, Kafri added. "We could easily modify the library to find peptides or small molecules with potential to act as inhibitors for a particular cellular state or pathway. Doing so would drastically accelerate the process of high-throughput drug design and testing, taking gene candidates from cell culture to the animal model."

The technique uses a genetically engineered HIV-1 virus, in which genes can be shuttled among bacteria, cell culture or animal models, in the same vehicle, or vector. This avoids time-consuming methods involved in gene isolation and amplification, such as the polymerase chain reaction, or PCR, procedure.

The method, designed by Kafri and gene therapy center researcher Hong Ma, enables scientists to rapidly screen cells for changes in a particular phenotype. They can easily isolate and identify the gene causing the changes and place it into an animal model or bacteria for further study.

The National Institutes of Health and the National Hemophilia Foundation supported this study.


Story Source:

The above story is based on materials provided by University Of North Carolina School Of Medicine. Note: Materials may be edited for content and length.


Cite This Page:

University Of North Carolina School Of Medicine. "Research Accelerates Discovery Of Novel Gene Function." ScienceDaily. ScienceDaily, 9 July 2004. <www.sciencedaily.com/releases/2004/07/040707090612.htm>.
University Of North Carolina School Of Medicine. (2004, July 9). Research Accelerates Discovery Of Novel Gene Function. ScienceDaily. Retrieved August 28, 2014 from www.sciencedaily.com/releases/2004/07/040707090612.htm
University Of North Carolina School Of Medicine. "Research Accelerates Discovery Of Novel Gene Function." ScienceDaily. www.sciencedaily.com/releases/2004/07/040707090612.htm (accessed August 28, 2014).

Share This




More Health & Medicine News

Thursday, August 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Mini Pacemaker Has No Wires

Mini Pacemaker Has No Wires

Ivanhoe (Aug. 27, 2014) Cardiac experts are testing a new experimental device designed to eliminate major surgery and still keep the heart on track. Video provided by Ivanhoe
Powered by NewsLook.com
After Cancer: Rebuilding Breasts With Fat

After Cancer: Rebuilding Breasts With Fat

Ivanhoe (Aug. 27, 2014) More than 269 million women are diagnosed with breast cancer each year. Many of them will need surgery and radiation, but there’s a new simple way to reconstruct tissue using a patient’s own fat. Video provided by Ivanhoe
Powered by NewsLook.com
Blood Clots in Kids

Blood Clots in Kids

Ivanhoe (Aug. 27, 2014) Every year, up to 200,000 Americans die from a blood clot that travels to their lungs. You’ve heard about clots in adults, but new research shows kids can get them too. Video provided by Ivanhoe
Powered by NewsLook.com
Radio Waves Knock out Knee Pain

Radio Waves Knock out Knee Pain

Ivanhoe (Aug. 27, 2014) Doctors have used radio frequency ablation or RFA to reduce neck and back pain for years. But now, that same technique is providing longer-term relief for patients with severe knee pain. Video provided by Ivanhoe
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins