Featured Research

from universities, journals, and other organizations

Scientists Develop Split Green For Tagging Protein

Date:
January 17, 2005
Source:
Los Alamos National Laboratory
Summary:
University of California scientists working at Los Alamos National Laboratory have developed a new protein tagging and detection system based on a process for "splitting" a green fluorescent protein.

Credit: Image Los Alamos National Laboratory

LOS ALAMOS, N.M., Jan. 3, 2004 - University of California scientists working at Los Alamos National Laboratory have developed a new protein tagging and detection system based on a process for "splitting" a green fluorescent protein. Unlike current protein detection methods, the method works both in living cells and in the test tube and can be used to quantify proteins down to 0.1 picomole, or one billionth of a gram of a typical protein molecule. Because the method can be used to detect protein aggregation within the living organisms, it will be useful for high-throughput studies of protein structure and protein production and for studying diseases, like Alzheimer's, that are associated with protein misfolding and aggregation.

In research published recently in the online version of the scientific journal Nature Biotechnology, Los Alamos scientists Stιphanie Cabantous, Tom Terwilliger and Geoff Waldo describe a method for engineering soluble, self-associating fragments of green fluorescent proteins that can be used to tag or detect soluble and insoluble proteins in living cells or cell lysates without changing protein solubility.

According to team member Geoff Waldo, "we think this discovery will have a major impact in the field of protein biotechnology and work related to deciphering the structure and function of proteins. I like to think of it as an enabling technology, a toolbox, if you will, for protein researchers, that could help them close the gap between sequencing the DNA of the human genome and determining the structures and functions of the encoded proteins."

The new system is based on the Rapid Protein Folding Assay (RPFA) method developed several years ago by Waldo, which used green fluorescence to signal protein folding. That method worked by fusing a protein's DNA to the DNA for green fluorescent proteins (GFP). The hybrid protein created by this linking then had the characteristics of both the GFP and the protein being assayed. If the protein being produced, or expressed, folds correctly, then the attached GFP also will fold correctly as it too is expressed. If the protein being expressed does not fold correctly, then the GFP also will not fold correctly and not fluoresce green. After scientists discovered that the GFP had some drawbacks, they developed the new system, which uses GFP fragments instead.

The split green fluorescent protein research resulted from Laboratory scientists efforts to develop a practical method for engineering protein folding and solubility as part of the National Institutes of Health (NIH) Protein Structure Initiative, a large-scale effort to determine the structures of thousands of protein molecules. These protein structures can be used in the design of new therapeutics and to deepen our understanding of how cells work.

###

Los Alamos is the lead institution in one of nine NIH-funded Protein Structure Initiative Centers. The Los Alamos center seeks to eradicate tuberculosis by solving questions regarding the structure of key proteins from Mycobacterium tuberculosis, which can then be targeted for drug-design efforts.


Story Source:

The above story is based on materials provided by Los Alamos National Laboratory. Note: Materials may be edited for content and length.


Cite This Page:

Los Alamos National Laboratory. "Scientists Develop Split Green For Tagging Protein." ScienceDaily. ScienceDaily, 17 January 2005. <www.sciencedaily.com/releases/2005/01/050111122854.htm>.
Los Alamos National Laboratory. (2005, January 17). Scientists Develop Split Green For Tagging Protein. ScienceDaily. Retrieved October 20, 2014 from www.sciencedaily.com/releases/2005/01/050111122854.htm
Los Alamos National Laboratory. "Scientists Develop Split Green For Tagging Protein." ScienceDaily. www.sciencedaily.com/releases/2005/01/050111122854.htm (accessed October 20, 2014).

Share This



More Matter & Energy News

Monday, October 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Hey, Doc! Sewage, Beer and Food Scraps Can Power Chevrolet’s Bi-Fuel Impala

Hey, Doc! Sewage, Beer and Food Scraps Can Power Chevrolet’s Bi-Fuel Impala

3BL Media (Oct. 20, 2014) — Hey, Doc! Sewage, Beer and Food Scraps Can Power Chevrolet’s Bi-fuel Impala Video provided by 3BL
Powered by NewsLook.com
Would A Travel Ban Even Work In Stopping Ebola Spread?

Would A Travel Ban Even Work In Stopping Ebola Spread?

Newsy (Oct. 19, 2014) — The U.S. currently isn't banning travel from Ebola-stricken areas, but it's at least being considered. Some argue though it could be counterproductive. Video provided by Newsy
Powered by NewsLook.com
Tech Giants Push Back After FBI Suggests Less Encryption

Tech Giants Push Back After FBI Suggests Less Encryption

Newsy (Oct. 19, 2014) — FBI Director James Comey's stance on encryption technology isn't receiving much support from the tech community. Video provided by Newsy
Powered by NewsLook.com
Microneedle Patch Promises Painless Pricks

Microneedle Patch Promises Painless Pricks

Reuters - Innovations Video Online (Oct. 18, 2014) — Researchers at The National University of Singapore have invented a new microneedle patch that could offer a faster and less painful delivery of drugs such as insulin and painkillers. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins