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Rapid, One-step, Ultra-sensitive Detection Of Food Poisoning Bacteria And Biothreats

Date:
April 16, 2007
Source:
American Chemical Society
Summary:
A new mosquito-sized biosensor can detect amazingly small amounts of disease-causing E. coli bacteria in food in a single-step process that takes only minutes, compared to hours required with conventional tests for that common food poisoning agent.

A new mosquito-sized biosensor can detect amazingly small amounts of disease-causing E. coli bacteria in food in a single-step process that takes only minutes, compared to hours required with conventional tests for that common food poisoning agent, researchers in Philadelphia are reporting.

The sensor also can quickly detect proteins important in medical diagnostic testing and very low levels of biothreats such as anthrax, according to the study, published in the April 1 edition of ACS' Analytical Chemistry, a semi-monthly journal.

In the study, Raj Mutharasan and colleagues point out that rapid measurements of very low concentrations of pathogens and proteins could have wide application in medical diagnostic testing, monitoring for biothreat agents, detecting contaminated food products and other areas. Existing tests for low-level pathogens, however, take time because they require a step to boost the concentration of microbes in a sample. No direct test currently exists for low-levels of proteins, the report adds.

The study describes use of the biosensor to detect E. coli in ground beef and other materials at some of the lowest concentrations ever reported. At the heart of the new biosensor is a vibrating cantilever, with a tiny beam supported at one end and coated with antibodies at its other, free-moving end. The antibodies are specific to the material being detected, such as E. coli, anthrax or proteins that are biomarkers for disease. When that antigen is present in a sample flowing through the biosensor, it binds to the cantilever and alters the frequency of vibration in a way that can be detected electronically.

American Chemical Society article in Analytical Chemistry: "Method for Label-Free Detection of Femtogram Quantities of Biologics in Flowing Liquid Samples"


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The above story is based on materials provided by American Chemical Society. Note: Materials may be edited for content and length.


Cite This Page:

American Chemical Society. "Rapid, One-step, Ultra-sensitive Detection Of Food Poisoning Bacteria And Biothreats." ScienceDaily. ScienceDaily, 16 April 2007. <www.sciencedaily.com/releases/2007/04/070416092206.htm>.
American Chemical Society. (2007, April 16). Rapid, One-step, Ultra-sensitive Detection Of Food Poisoning Bacteria And Biothreats. ScienceDaily. Retrieved August 1, 2014 from www.sciencedaily.com/releases/2007/04/070416092206.htm
American Chemical Society. "Rapid, One-step, Ultra-sensitive Detection Of Food Poisoning Bacteria And Biothreats." ScienceDaily. www.sciencedaily.com/releases/2007/04/070416092206.htm (accessed August 1, 2014).

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