Featured Research

from universities, journals, and other organizations

Winemaking Waste Proves Effective Against Disease-causing Bacteria In Early Studies

Date:
January 8, 2008
Source:
University of Rochester Medical Center
Summary:
A class of chemicals in red wine grapes may significantly reduce the ability of bacteria to cause cavities, according to a new study. The findings suggest that specific polyphenols, present in large amounts in fermented seeds and skins cast away after grapes are pressed, interfere with the ability of bacteria to contribute to tooth decay.

Grape skins used in making wine. New findings suggest that specific polyphenols, present in large amounts in fermented seeds and skins cast away after grapes are pressed, interfere with the ability of bacteria to contribute to tooth decay.
Credit: iStockphoto/Michael Westhoff

A class of chemicals in red wine grapes may significantly reduce the ability of bacteria to cause cavities, according to a study published recently in the Journal of Agricultural and Food Chemistry. The findings suggest that specific polyphenols, present in large amounts in fermented seeds and skins cast away after grapes are pressed, interfere with the ability of bacteria to contribute to tooth decay. Beyond cavities, the action of the wine grape-based chemicals may also hold clues for new ways to lessen the ability of bacteria to cause life-threatening, systemic infections.

Even better, the compounds embody an emerging philosophy in design of drugs against bacteria: take away their ability to cause disease without killing them. Current antibiotics often kill a strain of bacteria responsible for disease, only to create a vacuum quickly filled by related strains. The widespread overprescribing of antibiotics and the speed of bacterial evolution have greatly increased the likelihood that the strains most able to resist antibiotics will thrive and spread.

This trend is evident in recent reports that one strain of bacteria has become resistant to all 18 antibiotics approved for use in childhood ear infections, while another now causes more U.S. deaths than AIDS. New approaches seek to take away bacterial capabilities that cause disease (virulence factors) without "selecting for" resistance or killing beneficial bacteria.

"Most foods contain compounds that are both good and bad for dental health, so the message is not 'drink more wine to fight bacteria,'" said Hyun Koo, DDS, Ph.D., assistant professor of Dentistry within the Eastman Department of Dentistry and Center for Oral Biology at the Medical Center. "We hope to isolate the key compounds within the winemaking waste that render bad bacteria harmless, perhaps in the mouth with a new kind of rinse," said Koo, an author of the current study.

The findings are the result of collaboration between the University of Rochester Medical Center and the New York State Agricultural Experiment Station at Cornell University. Both institutions, with access to Finger Lakes wineries, have been looking at how compounds found in wine grapes impact human oral health. Together, they won a U.S. Department of Agriculture (USDA) grant in December 2005 to study the influence of grape polyphenols on oral bacteria, and today's publication is an early result.

Along with the potential value for medicine, the discovery that the waste products of winemaking may be useful in drug-making has economic implications. Grapes are one of the world's largest fruit crops, with more than 80 percent of grapes used to make wine. Fermented winemaking waste, called pomace, contains at least as many polyphenols as whole fruit, eliminating the need to use up perfectly good food to make any future drugs. Accordingly, the USDA is especially interested in the idea of bioprospecting in the winery versus the rainforest.

Study Details

Koo, who conducts his research at the Eastman Dental Center, concluded early in his career as an oral biologist that there are biochemical implications of foods on oral health -- other than to say "sugar is bad for teeth." In recent years, his and other labs have examined whether chemicals from cranberries, cocoa and grapes for instance have a therapeutic effect on bacterial pathogens. All contain polyphenols and the race is on to determine which may be the most useful medically.

The goal of the current study was to examine the make-up of polyphenols in red wine grape varieties and their ability to interfere with Streptococcus mutans (S. mutans), the bacteria that produces the substances most responsible for tooth decay: acid and the building blocks (glucans) of a dental biofilm called plaque.

Researchers prepared polyphenolic extracts from harvest season 2005 red wine grape varieties and pomace from wineries in the Finger Lakes region of New York state. These included Pinot Noir from Hosmer Winery in Ovid, N.Y., Cabernet Franc from Cornell Orchards in Lansing, N.Y., Baco Noir from Pleasant Valley Winery in Hammondsport, N.Y. and NoiretTM from Swedish Hill Winery in Romulus, N.Y. Varieties were pre-screened for their phenolic content, and grape pomace was chosen in general for its ready supply as an inexpensive source material. Red grapes have been shown to contain 40 percent more phenols content than white.

The team was most interested in examining the impact of grape polyphenols on two capabilities of S. mutans that enable it to thrive in the human mouth. First, it secretes enzymes known as glucosyltransferases (GTFs) that produce sugary, glue-like substances (glucans) that firmly attach bacteria to tooth surfaces and form a tough barrier around bacterial colonies. Such barriers, called the extracellular polysaccharide (EPS) matrix, protect the colony against environmental assaults, and make them, in some cases, hundreds of times more resistant to antibiotics. Bacteria living in these gunky fortresses are known as biofilms, whether they occur on teeth or elsewhere in the body. Many Streptococci (strep) and Staphylococci (staph) cause resistant forms of meningitis, pneumonia, staph aureus, as well as infections on heart valves and around stents, by forming biofilms. GTFs are a main virulence factor responsible for S. mutans biofilm formation, but other pathogens use similar mechanisms to produce EPS matrix. The hope is that learning about one will suggest ways to interfere with many.

A second linked set of virulence factors for S. mutans are its abilities to secrete acid, and to survive in that acid. Having evolved to be "acid durable," S. mutans can survive and out-compete other bacteria in the mouth. Better understanding of these mechanisms could also yield new ways to fight other biofilm related infections.

In the current study, researchers found that all polyphenol extracts inhibited two bacterial GTFs by as much as 85 percent (P<0.01)), a level of inhibition not previously observed in Koo's lab. Cabernet Franc extracts were more effective GTF inhibitors, with Pinot noir a close second at concentrations that might be useful therapeutically. Grape polyphenols were also found to cause S. mutans to produce significantly less acid.

This may be because they inhibit glycolysis, the process by which the bacteria turns sugar into energy also produces acid, researchers said. None of the extracts from any variety killed the bacteria outright. By targeting the ability of S. mutans to form EPS matrix, for example, therapeutic approaches to reducing the formation of biofilms could be precise and selective. Further chemical analysis will be needed to pinpoint which the most effective polythenol mix.

"Overall, the phenolic extracts disrupt essential virulence traits for a widespread, destructive oral pathogen, but without killing it," said Olga I. Padilla-Zakour, Ph.D., associate professor of Food Processing within the New York Agricultural Experiment Station of Cornell University. "We are excited about the potential application of active compounds from wine grape by-products in the control of biofilms as part of the precise targeting of bacterial disease."


Story Source:

The above story is based on materials provided by University of Rochester Medical Center. Note: Materials may be edited for content and length.


Cite This Page:

University of Rochester Medical Center. "Winemaking Waste Proves Effective Against Disease-causing Bacteria In Early Studies." ScienceDaily. ScienceDaily, 8 January 2008. <www.sciencedaily.com/releases/2008/01/080102122256.htm>.
University of Rochester Medical Center. (2008, January 8). Winemaking Waste Proves Effective Against Disease-causing Bacteria In Early Studies. ScienceDaily. Retrieved July 23, 2014 from www.sciencedaily.com/releases/2008/01/080102122256.htm
University of Rochester Medical Center. "Winemaking Waste Proves Effective Against Disease-causing Bacteria In Early Studies." ScienceDaily. www.sciencedaily.com/releases/2008/01/080102122256.htm (accessed July 23, 2014).

Share This




More Health & Medicine News

Wednesday, July 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Courts Conflicted Over Healthcare Law

Courts Conflicted Over Healthcare Law

AP (July 22, 2014) Two federal appeals courts issued conflicting rulings Tuesday on the legality of the federally-run healthcare exchange that operates in 36 states. (July 22) Video provided by AP
Powered by NewsLook.com
Why Do People Believe We Only Use 10 Percent Of Our Brains?

Why Do People Believe We Only Use 10 Percent Of Our Brains?

Newsy (July 22, 2014) The new sci-fi thriller "Lucy" is making people question whether we really use all our brainpower. But, as scientists have insisted for years, we do. Video provided by Newsy
Powered by NewsLook.com
Scientists Find New Way To Make Human Platelets

Scientists Find New Way To Make Human Platelets

Newsy (July 22, 2014) Boston scientists have discovered a new way to create fully functioning human platelets using a bioreactor and human stem cells. Video provided by Newsy
Powered by NewsLook.com
Gilead's $1000-a-Pill Drug Could Cure Hep C in HIV-Positive People

Gilead's $1000-a-Pill Drug Could Cure Hep C in HIV-Positive People

TheStreet (July 21, 2014) New research shows Gilead Science's drug Sovaldi helps in curing hepatitis C in those who suffer from HIV. In a medical study, the combination of Gilead's Hep C drug with anti-viral drug Ribavirin cured 76% of HIV-positive patients suffering from the most common hepatitis C strain. Hepatitis C and related complications have been a top cause of death in HIV-positive patients. Typical medication used to treat the disease, including interferon proteins, tended to react badly with HIV drugs. However, Sovaldi's %1,000-a-pill price tag could limit the number of patients able to access the treatment. TheStreet's Keris Lahiff reports from New York. Video provided by TheStreet
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins