Featured Research

from universities, journals, and other organizations

Biosensing Nanodevice To Revolutionize Health Screenings

Date:
March 27, 2008
Source:
Arizona State University
Summary:
One day soon a biosensing nanodevice may eliminate long lines at airport security checkpoints and revolutionize health screenings for diseases like anthrax, cancer and antibiotic resistant Staphylococcus aureus. Even more incredible than the device itself, is that it is based on the world's tiniest rotary motor: a biological engine measured on the order of molecules.

Composition of the nanodevice.
Credit: Wayne Frasch and David Spetzler

One day soon a biosensing nanodevice developed by Arizona State University researcher Wayne Frasch may eliminate long lines at airport security checkpoints and revolutionize health screenings for diseases like anthrax, cancer and antibiotic resistant Staphylococcus aureus (MRSA).

Even more incredible than the device itself, is that it is based on the world's tiniest rotary motor: a biological engine measured on the order of molecules.

Frasch works with the enzyme F1-adenosine triphosphatase, better known as F1- ATPase. This enzyme, only 10 to 12 nanometers in diameter, has an axle that spins and produces torque. This tiny wonder is part of a complex of proteins key to creating energy in all living things, including photosynthesis in plants. F1-ATPase breaks down adenosine triphosphate (ATP) to adenosine diphospahte (ADP), releasing energy. Previous studies of its structure and characteristics have been the source of two Nobel Prizes awarded in 1979 and 1997.

It was through his own detailed study of the rotational mechanism of the F1-ATPase, which operates like a three-cylinder Mazda rotary motor, that Frasch conceived of a way to take this tiny biological powerhouse and couple it with science applications outside of the human body.

An article authored by Frasch and his colleagues in the ASU School of Life Sciences details the technology that would allow this. Their publication "Single-molecule detection of DNA via sequence-specific links between F1-ATPase motors and gold nanorod sensors" was recently published in the journal Lab on a Chip, and featured in the online journal Chemical Biology.

What Frasch and his colleagues show is that the enzyme can be armed with an optical probe (gold nanorod) and manipulated to emit a signal when it detects a single molecule of target DNA. This is achieved by anchoring a quiescent F1-ATPase motor to a surface. A single strand of a reference biotinylated DNA molecule is then attached to its axle. The marker protein, biotin, on the DNA is known to bind specifically and tightly to the glycoprotein avidin, so an avidin-coated gold nanorod is then added. The avidin-nanorod attaches to the biotinylated DNA strand and forms a stable complex.

When a test solution containing a target piece of DNA is added, this DNA binds to the single complementary reference strand attached to the F1-ATPase. The DNA complex, suspended between the nanorod and the axle, forms a stiff bridge. Once ATP is added to the test solution, the F1-ATPase axle spins, and with it, the attached (now double-stranded) DNA and nanorod. The whirling nano-sized device emits a pulsing red signal that can then be detected with a microscope.

According to Frasch, the rotation discriminates fully assembled nanodevices from nonspecifically bound nanorods, resulting in a sensitivity limit of one zeptomole (600 molecules). Simply put, if it's not moving and flashing, it simply isn't relevant.

Moreover, Frasch says, "Studies with the F1-ATPase in my laboratory show that since it can detect single DNA molecules, it far exceeds the detection limits of conventional PCR [polymerase chain reaction] technology."

Such a detection instrument based on the F1-ATPase enzyme would also be "faster and more portable," he adds.

With support from Science Foundation Arizona (SFAz), Frasch will transfer his work from the bench to biotech, through establishment of a local company that utilizes the nano-sized F1-ATPase to produce a DNA detection instrument.

A prototype of the DNA detector is already in development. It is roughly the size of a small tissue box. Sampling would be as simple as taking a swab from an infected wound or a piece of baggage, dissolving it in a solution and placing a drop on a slide bearing reference F1-ATPases and their nanorods. Once in the instrument, red blinking signals emitted by rotating nanorods would let a computer know there's trouble, literally, in a flash.

SFAz funding has also enabled Frasch to extend the method to do protein detection at the single molecule level. This is novel because, unlike DNA, proteins can not be amplified artificially to improve the chances of detection.

"Rapid and sensitive biosensing of nucleic acids and proteins is vital for the identification of pathogenic agents of biomedical and bioterrorist importance," notes Frasch, who is also with the Center for Bioenergy and Photosynthesis in the College of Liberal Arts and Sciences. "It also provides a new avenue through which to analyze genotypes and forensic evidence."


Story Source:

The above story is based on materials provided by Arizona State University. Note: Materials may be edited for content and length.


Cite This Page:

Arizona State University. "Biosensing Nanodevice To Revolutionize Health Screenings." ScienceDaily. ScienceDaily, 27 March 2008. <www.sciencedaily.com/releases/2008/03/080325083344.htm>.
Arizona State University. (2008, March 27). Biosensing Nanodevice To Revolutionize Health Screenings. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/2008/03/080325083344.htm
Arizona State University. "Biosensing Nanodevice To Revolutionize Health Screenings." ScienceDaily. www.sciencedaily.com/releases/2008/03/080325083344.htm (accessed July 30, 2014).

Share This




More Matter & Energy News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Climate Change Could Cost Billions, According To White House

Climate Change Could Cost Billions, According To White House

Newsy (July 29, 2014) A report from the White House warns not curbing greenhouse gas emissions could cost the U.S. billions. Video provided by Newsy
Powered by NewsLook.com
Stranded Whale Watching Boat Returns to Boston

Stranded Whale Watching Boat Returns to Boston

Reuters - US Online Video (July 29, 2014) Passengers stuck overnight on a whale watching boat return safely to Boston. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Baluchistan Mining Eyes an Uncertain Future

Baluchistan Mining Eyes an Uncertain Future

AFP (July 29, 2014) Coal mining is one of the major industries in Baluchistan but a lack of infrastructure and frequent accidents mean that the area has yet to hit its potential. Duration: 01:58 Video provided by AFP
Powered by NewsLook.com
Easier Nuclear Construction Promises Fall Short

Easier Nuclear Construction Promises Fall Short

AP (July 29, 2014) The U.S. nuclear industry started building its first new plants using prefabricated Lego-like blocks meant to save time and prevent the cost overruns that crippled the sector decades ago. So far, it's not working. (July 29) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins