Featured Research

from universities, journals, and other organizations

A Novel Approach To Protein Variation In Synapses

Date:
April 9, 2008
Source:
American Society for Biochemistry and Molecular Biology
Summary:
Most brain functions, such as memory, require a sophisticated network of molecular interactions. However, experimental methods can only analyze a limited number of these interactions at a time. Now, researchers have pioneered a novel approach, which enables them to analyze hundreds of network molecules simultaneously. They were able to compare the relative amount and, importantly, the phosphorylation status of proteins in the synapses of four different brain regions.

Most brain functions, such as memory, require a sophisticated network of molecular interactions. However, experimental methods can only analyze a limited number of these interactions at a time.

Related Articles


Now, researchers have pioneered a novel approach, which enables them to analyze hundreds of network molecules simultaneously. Ralf Schoepfer, Al Burlingame and colleagues were able to compare the relative amount and, importantly, the phosphorylation status of proteins in the synapses of four different brain regions.

Synapses are the traffic intersections in our brain, the junctions between neurons where one cell passes information to its neighbor.

To better understand this flow of traffic, the scientists used mass spectrometry tools to analyze the post-synaptic density (PSD, the synapses' receiving end) of four brain regions in mice: cortex, midbrain, cerebellum, and hippocampus. In total, they examined over 2000 proteins and found some telling data about neuronal transmissions and memory.

For example, they observed that of all the brain regions, the hippocampus contained the highest levels of kinases and phosphatases, proteins that add and remove phosphate tags from other proteins. Phosphorylation provides a flexible and easily reversible way to regulate proteins, and this revelation suggests this may be how the hippocampus carries out one of its' main duties: collecting and consolidating memories.

This novel analysis should greatly aid efforts to understand how different parts of the brain handle their different jobs, and will also provides opportunities to investigate neuronal repair mechanisms and diseases such as Autism or Schizophrenia.

Journal reference: "Quantitative Analysis of Synaptic Phosphorylation and Protein Expression" by JC Trinidad, A Thalhammer, CG Specht, AJ Lynn, PR Baker, R Schoepfer, and AL Burlingame.Molecular & Cellular Proteomics. April 2008.


Story Source:

The above story is based on materials provided by American Society for Biochemistry and Molecular Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Biochemistry and Molecular Biology. "A Novel Approach To Protein Variation In Synapses." ScienceDaily. ScienceDaily, 9 April 2008. <www.sciencedaily.com/releases/2008/04/080404174806.htm>.
American Society for Biochemistry and Molecular Biology. (2008, April 9). A Novel Approach To Protein Variation In Synapses. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2008/04/080404174806.htm
American Society for Biochemistry and Molecular Biology. "A Novel Approach To Protein Variation In Synapses." ScienceDaily. www.sciencedaily.com/releases/2008/04/080404174806.htm (accessed December 18, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, December 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
UN: Up to One Million Facing Hunger in Ebola-Hit Countries

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

AFP (Dec. 17, 2014) Border closures, quarantines and crop losses in West African nations battling the Ebola virus could lead to as many as one million people going hungry, UN food agencies said on Wednesday. Duration: 00:52 Video provided by AFP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
Why Your Boss Should Let You Sleep In

Why Your Boss Should Let You Sleep In

Newsy (Dec. 17, 2014) According to research out of the University of Pennsylvania, waking up for work is the biggest factor that causes Americans to lose sleep. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins