Featured Research

from universities, journals, and other organizations

Novel Approach In Molecular Differentiation Of Prion Strains

Date:
August 30, 2008
Source:
Public Library of Science
Summary:
Scientists have identified a prion protein characteristic that is unique to some natural but unusual sheep scrapie cases. This finding may provide a novel method by which to study prion diversity and their possible changes during cross-species transmission.

A team from the French Food Safety Agency, Lyon, France, has identified a prion protein characteristic that is unique to some natural but unusual sheep scrapie cases. This finding may provide a novel method by which to study prion diversity and their possible changes during cross-species transmission.

Mystery still surrounds the origin of the transmissible agent involved in the food-borne epidemic of bovine spongiform encephalopathy (BSE). Classical BSE, more commonly known as mad cow disease, is a known cause of a variant form of the incurable, degenerative neurological disorder Creutzfeldt-Jakob disease in humans. It has recently been proposed that this could have been the result of the recycling of an atypical, more probably sporadic form of BSE (called bovine amyloidotic spongiform encephalopathy, or L-type BSE) in an intermediate host, such as sheep.

The team, led by Thierry Baron, analyzed the molecular features of the disease-associated protease-resistant prion protein (PrPres) to determine any differences which might discriminate between scrapie and BSE cases. The researchers sampled PrPres from the brains of transgenic mice overexpressing the ovine prion protein after experimental infection with prions from bovine classical BSE, L-type BSE, and ovine scrapie. Scrapie cases were found to include rare ''CH1641-like'' isolates, which share some PrPres molecular features with classical BSE and L-type BSE.

The molecular features of the prion protein in the "CH1641-like" sheep scrapie cases more closely resemble those found in L-type BSE compared to classical BSE. However, from a series of four "CH1641-like" scrapie cases, the researchers found a pathological C-terminal prion protein product that was undetectable from both L-type and classical BSE transmitted to such mice, clearly suggesting that such scrapie isolates are not linked to these BSE forms.

Further studies to confirm this discriminating factor are needed in sheep, especially from sheep experimentally infected with L-type BSE, which were not available for this study. These findings, however, add a novel approach in the molecular differentiation of prion strains and may help to better understand their possible changes during cross-species transmissions.


Story Source:

The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Baron et al. A C-Terminal Protease-Resistant Prion Fragment Distinguishes Ovine “CH1641-Like” Scrapie from Bovine Classical and L-Type BSE in Ovine Transgenic Mice. PLoS Pathogens, 2008; 4 (8): e1000137 DOI: 10.1371/journal.ppat.1000137

Cite This Page:

Public Library of Science. "Novel Approach In Molecular Differentiation Of Prion Strains." ScienceDaily. ScienceDaily, 30 August 2008. <www.sciencedaily.com/releases/2008/08/080828220507.htm>.
Public Library of Science. (2008, August 30). Novel Approach In Molecular Differentiation Of Prion Strains. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/2008/08/080828220507.htm
Public Library of Science. "Novel Approach In Molecular Differentiation Of Prion Strains." ScienceDaily. www.sciencedaily.com/releases/2008/08/080828220507.htm (accessed July 30, 2014).

Share This




More Plants & Animals News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Otters Enjoy Water Slides at Japan Zoo

Raw: Otters Enjoy Water Slides at Japan Zoo

AP (July 30, 2014) River otters were hitting the water slides to beat the summer heatwave on Wednesday at Ichikawa City's Zoological and Botanical Garden. (July 30) Video provided by AP
Powered by NewsLook.com
In Virginia, the Rise of a New Space Coast

In Virginia, the Rise of a New Space Coast

AP (July 30, 2014) Every summer, tourists make the pilgrimage to Chincoteague Island, Va. to see wild ponies cross the Assateague Channel. But, it's the rockets sending to supplies to the International Space Station that are making this a year-round destination. (July 30) Video provided by AP
Powered by NewsLook.com
Rodents Rampant in Gardens Around Louvre

Rodents Rampant in Gardens Around Louvre

AP (July 29, 2014) Food scraps and other items left on the grounds by picnickers brings unwelcome visitors to the grounds of the world famous and popular Louvre Museum in Paris. (July 29) Video provided by AP
Powered by NewsLook.com
Jane Goodall Warns Great Apes Face Extinction

Jane Goodall Warns Great Apes Face Extinction

AFP (July 29, 2014) The world's great apes face extinction within decades, renowned chimpanzee expert Jane Goodall warned Tuesday in a call to arms to ensure man's closest relatives are not wiped out. Duration: 00:58 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins