Featured Research

from universities, journals, and other organizations

Purifying Parasites From Host Cells With Light

Date:
September 16, 2008
Source:
American Society for Biochemistry and Molecular Biology
Summary:
Researchers have developed a clever method to purify parasitic organisms from their host cells, which will allow for more detailed studies and a deeper insight into the biology of organisms that cause millions of cases of disease each year.

Electron micrographs (left=low, right=high magnification) highlighting the purification of Leishmania parasites (dark shapes) from their host cells.
Credit: MCP

Researchers have developed a clever method to purify parasitic organisms from their host cells, which will allow for more detailed proteomic studies and a deeper insight into the biology of organisms that cause millions of cases of disease each year.

Related Articles


Many infectious pathogens, like those that cause Toxoplasmosis or Leishmaniases, have a complex life cycle alternating between free-living creature and cell-enclosed parasite. A thorough analysis of the proteins that help these organisms undergo this lifestyle change would be tremendously useful for drug or vaccine development; however, it's extremely difficult to separate the parasites from their host cell for detailed study.

As reported in the September Molecular & Cellular Proteomics, Toni Aebischer and colleagues worked around this problem by designing special fluorescent Leishmania mexicana (one of the many Leishmaniases parasites). They then passed infected cells through a machine that can separate cell components based on how much they glow. Using this approach, the researchers separated the Leishmania parasites with only about 2% contamination, far better than current methods.

They then successfully identified 509 proteins in the parasites, 34 of which were more prominent in parasites than free –living Leishmania. The results yielded many characteristics of these organisms, such as a high presence of fatty acid degrading enzymes, which highlights adaptation to intracellularly available energy sources. The identified proteins should provide a good data set for continued selection of drug targets, and the success of this method should make it a good resource for other cellular parasites like malaria.


Story Source:

The above story is based on materials provided by American Society for Biochemistry and Molecular Biology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Daniel Paape, Christoph Lippuner, Monika Schmid, Renate Ackermann, Martin E. Barrios-Llerena, Ursula Zimny-Arndt, Volker Brinkmann, Benjamin Arndt, Klaus Peter Pleissner, Peter R. Jungblut, and Toni Aebischer. Transgenic, Fluorescent Leishmania mexicana Allow Direct Analysis of the Proteome of Intracellular Amastigotes. Molecular & Cellular Proteomics, 2008; 7 (9): 1688 DOI: 10.1074/mcp.M700343-MCP200

Cite This Page:

American Society for Biochemistry and Molecular Biology. "Purifying Parasites From Host Cells With Light." ScienceDaily. ScienceDaily, 16 September 2008. <www.sciencedaily.com/releases/2008/09/080912132958.htm>.
American Society for Biochemistry and Molecular Biology. (2008, September 16). Purifying Parasites From Host Cells With Light. ScienceDaily. Retrieved March 27, 2015 from www.sciencedaily.com/releases/2008/09/080912132958.htm
American Society for Biochemistry and Molecular Biology. "Purifying Parasites From Host Cells With Light." ScienceDaily. www.sciencedaily.com/releases/2008/09/080912132958.htm (accessed March 27, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, March 27, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Jockey Motion Tracking Reveals Racing Prowess

Jockey Motion Tracking Reveals Racing Prowess

Reuters - Innovations Video Online (Mar. 26, 2015) Using motion tracking technology, researchers from the Royal Veterinary College (RVC) are trying to establish an optimum horse riding style to train junior jockeys, as well as enhance safety, health and well-being of both racehorses and jockeys. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Botswana Talks to End Illegal Wildlife Trade

Botswana Talks to End Illegal Wildlife Trade

AFP (Mar. 25, 2015) Experts are gathering in Botswana to try to end the illegal wildlife trade that is decimating populations of elephants, rhinos and other threatened species. Duration: 01:05 Video provided by AFP
Powered by NewsLook.com
Elephants Help Keep 18-Wheeler From Toppling Over

Elephants Help Keep 18-Wheeler From Toppling Over

Newsy (Mar. 25, 2015) The Natchitoches Parish Sheriff&apos;s Office discovered two elephants keeping a tractor-trailer that had gotten stuck in some mud upright on a highway. Video provided by Newsy
Powered by NewsLook.com
Baby 'pet' Orangutan Rescued from Chicken Cage Takes First Steps

Baby 'pet' Orangutan Rescued from Chicken Cage Takes First Steps

Reuters - Light News Video Online (Mar. 25, 2015) Buti, a baby orangutan who was left malnourished in a chicken cage before his rescue, takes his first steps after months of painful physical therapy. Mana Rabiee reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins