Featured Research

from universities, journals, and other organizations

Purifying Parasites From Host Cells With Light

Date:
September 16, 2008
Source:
American Society for Biochemistry and Molecular Biology
Summary:
Researchers have developed a clever method to purify parasitic organisms from their host cells, which will allow for more detailed studies and a deeper insight into the biology of organisms that cause millions of cases of disease each year.

Electron micrographs (left=low, right=high magnification) highlighting the purification of Leishmania parasites (dark shapes) from their host cells.
Credit: MCP

Researchers have developed a clever method to purify parasitic organisms from their host cells, which will allow for more detailed proteomic studies and a deeper insight into the biology of organisms that cause millions of cases of disease each year.

Related Articles


Many infectious pathogens, like those that cause Toxoplasmosis or Leishmaniases, have a complex life cycle alternating between free-living creature and cell-enclosed parasite. A thorough analysis of the proteins that help these organisms undergo this lifestyle change would be tremendously useful for drug or vaccine development; however, it's extremely difficult to separate the parasites from their host cell for detailed study.

As reported in the September Molecular & Cellular Proteomics, Toni Aebischer and colleagues worked around this problem by designing special fluorescent Leishmania mexicana (one of the many Leishmaniases parasites). They then passed infected cells through a machine that can separate cell components based on how much they glow. Using this approach, the researchers separated the Leishmania parasites with only about 2% contamination, far better than current methods.

They then successfully identified 509 proteins in the parasites, 34 of which were more prominent in parasites than free –living Leishmania. The results yielded many characteristics of these organisms, such as a high presence of fatty acid degrading enzymes, which highlights adaptation to intracellularly available energy sources. The identified proteins should provide a good data set for continued selection of drug targets, and the success of this method should make it a good resource for other cellular parasites like malaria.


Story Source:

The above story is based on materials provided by American Society for Biochemistry and Molecular Biology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Daniel Paape, Christoph Lippuner, Monika Schmid, Renate Ackermann, Martin E. Barrios-Llerena, Ursula Zimny-Arndt, Volker Brinkmann, Benjamin Arndt, Klaus Peter Pleissner, Peter R. Jungblut, and Toni Aebischer. Transgenic, Fluorescent Leishmania mexicana Allow Direct Analysis of the Proteome of Intracellular Amastigotes. Molecular & Cellular Proteomics, 2008; 7 (9): 1688 DOI: 10.1074/mcp.M700343-MCP200

Cite This Page:

American Society for Biochemistry and Molecular Biology. "Purifying Parasites From Host Cells With Light." ScienceDaily. ScienceDaily, 16 September 2008. <www.sciencedaily.com/releases/2008/09/080912132958.htm>.
American Society for Biochemistry and Molecular Biology. (2008, September 16). Purifying Parasites From Host Cells With Light. ScienceDaily. Retrieved March 3, 2015 from www.sciencedaily.com/releases/2008/09/080912132958.htm
American Society for Biochemistry and Molecular Biology. "Purifying Parasites From Host Cells With Light." ScienceDaily. www.sciencedaily.com/releases/2008/09/080912132958.htm (accessed March 3, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Tuesday, March 3, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

500 Snakes Surprise Construction Workers In Canada

500 Snakes Surprise Construction Workers In Canada

Newsy (Mar. 2, 2015) Hundreds of snakes, disturbed by a construction project, were relocated to a wildlife rescue association in Canada. Video provided by Newsy
Powered by NewsLook.com
Going Gluten-Free Could Get You A Tax Break

Going Gluten-Free Could Get You A Tax Break

Newsy (Mar. 2, 2015) If a doctor advises you to remove gluten from your diet, you could get a tax deduction on the amount you spend on gluten-free foods. Video provided by Newsy
Powered by NewsLook.com
Zookeepers Copy Animal Poses In Hilarious Viral Photos

Zookeepers Copy Animal Poses In Hilarious Viral Photos

Buzz60 (Mar. 2, 2015) Zookeepers at the Symbio Wildlife Park in Helensburgh, Australia decided to take some of their favorite animal photos and recreate them by posing just like the animals. Jen Markham (@jenmarkham) has the story. Video provided by Buzz60
Powered by NewsLook.com
Heavy Toll as Australian Farmers Struggle Through Drought

Heavy Toll as Australian Farmers Struggle Through Drought

AFP (Mar. 2, 2015) Mounting debts, despair and forced repossessions are taking a heavy toll on farmers in parts of Australia suffering from its worst drought in 100 years. Duration: 02:16 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins