Featured Research

from universities, journals, and other organizations

Genetic Mutation That May Predict Organ Rejection Identified

Date:
September 16, 2008
Source:
Children's Hospital of Pittsburgh
Summary:
Using a novel combination of cutting-edge technologies to scan the human genome, researchers have identified a genetic mutation that identifies transplant recipients who experience rejection.

Using a novel combination of cutting-edge technologies to scan the human genome, researchers at Children's Hospital of Pittsburgh of UPMC have identified a genetic mutation that identifies transplant recipients who experience rejection.

Known as a single nucleotide polymorphism (SNP), the genetic mutation validates the effectiveness of the system the researchers developed to search the human genome, according to principal investigator Rakesh Sindhi, MD, director of Pediatric Transplant Research in the Hillman Center for Pediatric Transplantation at Children's Hospital. They studied DNA samples from 80 children who received liver transplants and their parents.

"To identify mutations that mark a disease, from the millions of known mutations in the human genome, one needs to study hundreds, even thousands of patients with that disease. As a result, large-scale scanning of known mutations has not been applied to rarer diseases, such as those that affect children. However, by combining multiple layers of genetic information, with information from the cell types and processes affected by these genes, we can now study less common diseases using smaller numbers of subjects," Dr. Sindhi said. "Such mutations are likely to become the basis of a genomic fingerprint, which will allow us to predict who will experience rejection beforehand, and to personalize antirejection medication. The novel combination of techniques used in this study is a major methodological advance toward developing personalized diagnostics for transplant recipients, which will improve outcomes and quality of life."

The approach used by Dr. Sindhi and colleagues involves powerful new tools known as microarrays. Over 500,000 known mutations spanning the entire genome are evaluated in one type of microarray. The substructure of gene products known as ribonucleic acid, or RNA, is evaluated for all known genes in another type of microarray. The mutation associated with rejection was first identified by comparing known mutations in children who received liver transplantation or were given a particular antirejection regimen, with those from their biological parents. Therefore, this study also provides evidence for the inherited basis of rejection and rejection-free outcomes on a given anti-rejection regimen.

Studying antirejection medications is important because, while they make transplantation possible, they also can have side effects such as infections and cancers, some of which can be life-threatening.

"By establishing a genomic fingerprint for rejection, and applying personalized antirejection strategies before the transplant even occurs, we are hopeful we can reduce rejection rates and drug-induced side effects for children with liver transplants, from 50 percent to 20 percent or less," Dr. Sindhi said.

Results of the study are published in the September issue of Gastroenterology, the official publication of the American Gastroenterological Association.

Sindhi's research is supported by the National Institute of Allergy and Infectious Diseases, the Children's Hospital of Pittsburgh Foundation, and the Hillman Foundation. Study collaborators include Daniel E. Weeks, PhD, and associates from the Department of Human Genetics at the University of Pittsburgh, and Hakon Hakonarson, MD, from the Center for Applied Genomics at The Children's Hospital of Philadelphia.


Story Source:

The above story is based on materials provided by Children's Hospital of Pittsburgh. Note: Materials may be edited for content and length.


Cite This Page:

Children's Hospital of Pittsburgh. "Genetic Mutation That May Predict Organ Rejection Identified." ScienceDaily. ScienceDaily, 16 September 2008. <www.sciencedaily.com/releases/2008/09/080915132702.htm>.
Children's Hospital of Pittsburgh. (2008, September 16). Genetic Mutation That May Predict Organ Rejection Identified. ScienceDaily. Retrieved April 17, 2014 from www.sciencedaily.com/releases/2008/09/080915132702.htm
Children's Hospital of Pittsburgh. "Genetic Mutation That May Predict Organ Rejection Identified." ScienceDaily. www.sciencedaily.com/releases/2008/09/080915132702.htm (accessed April 17, 2014).

Share This



More Health & Medicine News

Thursday, April 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Could Even Casual Marijuana Use Alter Your Brain?

Could Even Casual Marijuana Use Alter Your Brain?

Newsy (Apr. 16, 2014) A new study conducted by researchers at Northwestern and Harvard suggests even casual marijuana use can alter your brain. Video provided by Newsy
Powered by NewsLook.com
Thousands Of Vials Of SARS Virus Go Missing

Thousands Of Vials Of SARS Virus Go Missing

Newsy (Apr. 16, 2014) A research institute in Paris somehow misplaced more than 2,000 vials of the deadly SARS virus. Video provided by Newsy
Powered by NewsLook.com
Formerly Conjoined Twins Released From Dallas Hospital

Formerly Conjoined Twins Released From Dallas Hospital

Newsy (Apr. 16, 2014) Conjoined twins Emmett and Owen Ezell were separated by doctors in August. Now, nearly nine months later, they're being released from the hospital. Video provided by Newsy
Powered by NewsLook.com
Ebola Outbreak Now Linked To 121 Deaths

Ebola Outbreak Now Linked To 121 Deaths

Newsy (Apr. 15, 2014) The ebola virus outbreak in West Africa is now linked to 121 deaths. Health officials fear the virus will continue to spread in urban areas. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins