Featured Research

from universities, journals, and other organizations

Protein Shown To Be Major Component Of Synapse Construction

Date:
December 26, 2008
Source:
Rockefeller University Press
Summary:
Nitric oxide gets neurons together. And it seems to do it backward. New research suggests that a protein called PSD-95 prompts nitric oxide release from postsynaptic dendritic spines, prompting nearby presynaptic axons to lock on, and develop new synapses.

More PSD-95 means bigger spines (top), and multiple axon connections.
Credit: Nikonenko, I., et al. 2008. J. Cell Biol. doi:10.1083/jcb.200805132

Nitric oxide gets neurons together. And it seems to do it backward. Work by Nikonenko et al. suggests that a protein called PSD-95 prompts nitric oxide release from postsynaptic dendritic spines, prompting nearby presynaptic axons to lock on, and develop new synapses.

Related Articles


The study will appear in the December 15, 2008 issue of The Journal of Cell Biology (JCB).

It is becoming increasingly clear that synaptogenesis is not solely axon driven. PSD-95 is a major component of postsynaptic densities—a conglomeration of scaffolding proteins, neurotransmitter receptors, and signaling proteins that are thought to shape dendritic spines—and reduced levels of PSD-95 impair synapse development. How PSD-95 works, however, was unknown.

Nikonenko et al. overexpressed PSD-95 in cultured hippocampal neurons and found that the cells' dendritic spines grew two to three times their normal size and were often contacted by multiple axons—a rare occurrence in the adult brain. By mutating different parts of PSD-95, the team discovered that the region responsible for prompting multi-axon connections was also required for binding nitrogen oxide synthase. The team cut to the chase, bathed neurons in nitric oxide, and showed this was sufficient to promote the extra axon connections. Since bathing cells in nitric oxide and overexpressing proteins do not reflect normal physiological conditions, the team also inhibited nitric oxide synthase in wild-type neurons and confirmed that synapse density was reduced.

Overexpressing PSD-95 increased the amount of nitric oxide synthase at postsynaptic densities, suggesting PSD-95 recruits the synthase to its required locale. Interestingly, PSD-95 that lacked its synthase interaction domain still induced super-sized dendritic spines, suggesting PSD-95 wears more than one hat at the synapse construction site.


Story Source:

The above story is based on materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Cite This Page:

Rockefeller University Press. "Protein Shown To Be Major Component Of Synapse Construction." ScienceDaily. ScienceDaily, 26 December 2008. <www.sciencedaily.com/releases/2008/12/081215111133.htm>.
Rockefeller University Press. (2008, December 26). Protein Shown To Be Major Component Of Synapse Construction. ScienceDaily. Retrieved February 27, 2015 from www.sciencedaily.com/releases/2008/12/081215111133.htm
Rockefeller University Press. "Protein Shown To Be Major Component Of Synapse Construction." ScienceDaily. www.sciencedaily.com/releases/2008/12/081215111133.htm (accessed February 27, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Friday, February 27, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Could a $34 Smartphone Device Improve HIV Diagnosis in Africa?

Reuters - Innovations Video Online (Feb. 27, 2015) A dongle that plugs into a Smartphone mimics a lab-based blood test for HIV and syphilis and can detect the diseases in 15 minutes, say researchers. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Doctor Says Head Transplants Possible Within Two Years

Doctor Says Head Transplants Possible Within Two Years

Buzz60 (Feb. 27, 2015) An Italian doctor is saying he could stick someone&apos;s head onto someone else&apos;s body. Patrick Jones (@Patrick_E_Jones) reports. Video provided by Buzz60
Powered by NewsLook.com
How Your Dentist Could Help Screen You For Diabetes

How Your Dentist Could Help Screen You For Diabetes

Newsy (Feb. 27, 2015) A new study from researchers at New York University suggests dentists could soon use blood samples taken from patients&apos; mouths to test for diabetes. Video provided by Newsy
Powered by NewsLook.com
The Best Tips to Makeover Your Health

The Best Tips to Makeover Your Health

Buzz60 (Feb. 27, 2015) If you&apos;re looking to boost your health this season, there are a few quick and easy steps to prompt you for success. Krystin Goodwin (@Krystingoodwin) has the best tips to give your health a makeover this spring! Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins