Featured Research

from universities, journals, and other organizations

Zebrafish Cloning Methods Improved

Date:
September 1, 2009
Source:
Michigan State University
Summary:
Researchers have developed a new, more efficient way of cloning zebra fish, a breakthrough that could have implications for human health research.

These are zebrafish that were cloned in the Michigan State University Cellular Reprogramming Laboratory.
Credit: G.L. Kohuth, MSU University Relations

A team of Michigan State University researchers has developed a new, more efficient way of cloning zebra fish, a breakthrough that could have implications for human health research.

Related Articles


The work of the MSU researchers, detailed in the recent issue of the journal Nature Methods, is important because zebra fish, small striped fish common to many aquariums, are quickly becoming the animal of choice for many researchers.

"After the mouse, it is the most commonly used vertebrate in genetic studies," said Jose Cibelli, an MSU professor of animal science and one of the paper's co-authors. "It is used in cancer research and cardiovascular research because they have many of the same genes we have."

For more than 20 years, Cibelli said, zebra fish have also served as an excellent model for understanding normal development and birth defects. More recently, research with zebra fish has extended to model human diseases and to analyze the formation and functions of cell populations within organs.

Previous methods of cloning zebra fish have had very low success rates. But using this new method, the number of cloned fish that can be obtained from an adult fin cell or an embryonic tail clip increased by 2 percent to 13 percent respectively.

The normal cloning technique uses an unfertilized egg and a donor cell. The DNA is removed from the egg and replaced by the DNA taken from the donor. The egg cell is then coaxed into dividing. The resulting fish is an exact genetic copy of the donor.

Using Cibelli and colleagues' technique, the egg is gently removed from the female zebra fish and placed in a solution of ovarian fluid from a Chinook salmon.

"This worked well," he said, "because it kept the egg inactive for some time. It gave us two or three hours to work with.

"It was also useful that we are working in Michigan. The state's Department of Natural Resources was very generous in helping us collect fluid from female Chinooks."

Then, borrowing a technique from human in vitro fertilization, the DNA was removed from the egg by using a laser.

Next, Cibelli's team devised a novel, more efficient way of transplanting the donor cells into the egg.

"The tricky part was finding a way to get into the egg," he said. "We used the same entrance that sperm uses. There was only one spot on the egg, and we had to find it."

What makes zebra fish so useful in research is their eggs are transparent and the fish's development is easy to follow.

Improving on the techniques of zebra fish cloning also is important because currently only the mouse remains the best model for gene targeting.

"So far the mouse is the only one from which you can delete genes in a reliable fashion," Cibelli said. "What researchers do is mutate a gene, abolish its function completely, and then study the consequences."


Story Source:

The above story is based on materials provided by Michigan State University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Kannika Siripattarapravat et al. Novel Somatic Cell Nuclear Transfer Method in Zebra Fish. Nature Methods, August 30, 2009

Cite This Page:

Michigan State University. "Zebrafish Cloning Methods Improved." ScienceDaily. ScienceDaily, 1 September 2009. <www.sciencedaily.com/releases/2009/08/090830192034.htm>.
Michigan State University. (2009, September 1). Zebrafish Cloning Methods Improved. ScienceDaily. Retrieved March 6, 2015 from www.sciencedaily.com/releases/2009/08/090830192034.htm
Michigan State University. "Zebrafish Cloning Methods Improved." ScienceDaily. www.sciencedaily.com/releases/2009/08/090830192034.htm (accessed March 6, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, March 6, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Giant Panda Goes Walkabout in Southwest China

Giant Panda Goes Walkabout in Southwest China

AFP (Mar. 6, 2015) — A giant panda goes walkabout alone at night in southwest China. Duration: 00:37 Video provided by AFP
Powered by NewsLook.com
Nesting Bald Eagle Covered in Snow Up to Its Neck

Nesting Bald Eagle Covered in Snow Up to Its Neck

Buzz60 (Mar. 6, 2015) — The Pennsylvania State Game Commission captured amazing shots of a nesting bald eagle who stayed on its nest during a snowstorm, even when the snow piled all the way up to its neck. Jen Markham (@jenmarkham) has the story. Video provided by Buzz60
Powered by NewsLook.com
Lack of Snow Pushes Alaska Sled Dog Race North

Lack of Snow Pushes Alaska Sled Dog Race North

AP (Mar. 6, 2015) — A shortage of snow has forced Alaska&apos;s Iditarod Trail Sled Dog Race to move 300 miles north to Fairbanks. The ceremonial start through downtown Anchorage will take place this weekend, using snow stockpiled earlier this winter. (March 6) Video provided by AP
Powered by NewsLook.com
Praying Mantis Looks Long Before It Leaps

Praying Mantis Looks Long Before It Leaps

Reuters - Innovations Video Online (Mar. 5, 2015) — Slowed-down footage of the leaps of praying mantises show the insect&apos;s extraordinary precision, say researchers. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins