Resistant wheat goes for the gut to protect against Hessian flies

Hessian fly larvae midguts -- similar to human intestines -- were observed in three different feeding situations. Larvae on susceptible plants had normal midgut function. Those that were given nothing to eat showed no damage to the midgut, though they starved. But those on plants resistant to Hessian flies showed serious midgut disruption.

Richard Shukle and Christie Williams, research scientists with the USDA Agricultural Research Service Crop Production and Pest Control Research Unit and Purdue adjunct associate professors of entomology, and Subhashree Subramanyam, a research associate in agronomy, had their findings published in the early online edition of the Journal of Insect Physiology.

Shukle said that within three hours, larvae feeding on resistant wheat had abnormal microvilli, fingerlike appendages that extend inward from the walls of the midgut to increase surface area for nutrient absorption. By six hours, the microvilli were nearly destroyed and the midgut was void of food.

"Some chemical or compound they're encountering from the resistant plant is causing the microvilli to become disrupted, and it's happening very quickly," Shukle said. "The midgut is certainly one of the major targets of the defense compounds elicited from a resistant plant. There could be other targets, but the midgut is a major target."

The research also showed that Hessian fly midguts are lacking a peritrophic membrane, a common insect feature that protects the microvilli from toxic substances.

Hessian flies cause considerable damage to wheat crops, with grain yields reduced as much as 20 bushels per acre with moderate infestation levels. There are about a half dozen undeployed genes identified in wheat that offer high resistance to Hessian flies and could be deployed to defend the plants. But understanding how the resistance genes stave off Hessian flies is important because the fly has overcome or is overcoming several genes initially deployed to protect wheat lines.

Williams and Subramanyam believe lectins could be one of the toxic substances causing the midgut disruption. Lectins are proteins that bind carbohydrates.

Williams said resistant wheat plants contain higher levels of proteins made by the Hessian fly responsive genes, called Hfr-1 and Hfr-3, than susceptible plants. Studies have shown that the protein made by Hfr-1 is a lectin, and the protein made by Hfr-3 is lectin-like.

The fly larvae activate the genes responsible for resistance, and Williams and Subramanyam believe those genes may be triggering lectin function.

"Lectins are one of the toxins in resistant plants, and they are probably targeting the microvilli," Subramanyam said.

Shukle said he will next try to determine what is attaching to the microvilli causing the disruption and will test the Hfr-1 protein to see if it is toxic to the flies.

The USDA ARS Crop Production and Pest Control Research Unit funded the research.