Featured Research

from universities, journals, and other organizations

New tool for RNA silencing

Date:
April 2, 2010
Source:
BioMed Central
Summary:
Antisense reagents have been developed for C. Elegans micro RNA. Researchers have created the first class of reagents to potently and selectively inhibit miRNAs in this widely used model organism.

Antisense reagents have been developed for C. elegans micro RNA. Researchers writing in BioMed Central's open access journal Silence have created the first class of reagents to potently and selectively inhibit miRNAs in this widely used model organism.

Wen-hong Li, from the University of Texas Southwestern Medical Center, USA, worked with a team of researchers including Dr. Genhua Zheng and Dr. Victor Ambros (University of Massachusetts Medical School) to develop this latest addition to the genetics toolkit. He said, "Caenorhabditis elegans has long been used as a model organism for studying the regulation and function of small non-coding RNA molecules, and yet no antisense reagents have been available to reliably inhibit miRNAs in worms. Our fluorescently labeled reagents were synthesized by conjugating dextran with 2'-O-methyl oligoribonucleotide, and can be conveniently introduced into the germline of adult hermaphrodites and are transmitted to their progeny."

Li's team found that their new reagents efficiently and specifically inhibited targeted miRNA in different tissues, including the hypodermis, the vulva and the nervous system. They can be used combinatorially to inhibit more than one miRNA in the same animal. They conclude, "Combined with numerous mutants or reporter stains available, these reagents should provide a convenient approach to examine genetic interactions that involve miRNA, and may facilitate studying functions of miRNAs, especially ones whose deletion strains are difficult to generate. Further, the remarkable efficacy of these antisense reagents seen in worms also suggested C. elegans as a powerful, convenient, and economical biological system to facilitate developing new chemistry and novel probes for studying miRNA and other small non-coding RNAs."


Story Source:

The above story is based on materials provided by BioMed Central. Note: Materials may be edited for content and length.


Journal References:

  1. Genhua Zheng, Victor Ambros and Wen-hong Li. Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent. Silence, 2010; DOI: 10.1186/1758-907X-1-9
  2. Samrat T Kundu, Frank J Slack. Robust and specific inhibition of microRNAs in Caenorhabditis elegans. Journal of Biology, 2010; 9 (3): 20 DOI: 10.1186/jbiol230

Cite This Page:

BioMed Central. "New tool for RNA silencing." ScienceDaily. ScienceDaily, 2 April 2010. <www.sciencedaily.com/releases/2010/04/100401101001.htm>.
BioMed Central. (2010, April 2). New tool for RNA silencing. ScienceDaily. Retrieved April 23, 2014 from www.sciencedaily.com/releases/2010/04/100401101001.htm
BioMed Central. "New tool for RNA silencing." ScienceDaily. www.sciencedaily.com/releases/2010/04/100401101001.htm (accessed April 23, 2014).

Share This



More Plants & Animals News

Wednesday, April 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Monkeys Are Better At Math Than We Thought, Study Shows

Monkeys Are Better At Math Than We Thought, Study Shows

Newsy (Apr. 23, 2014) A Harvard University study suggests monkeys can use symbols to perform basic math calculations. Video provided by Newsy
Powered by NewsLook.com
Raw: Leopard Bites Man in India

Raw: Leopard Bites Man in India

AP (Apr. 22, 2014) A leopard caused panic in the city of Chandrapur on Monday when it sprung from the roof of a house and charged at rescue workers. (April 22) Video provided by AP
Powered by NewsLook.com
Iowa College Finds Beauty in Bulldogs

Iowa College Finds Beauty in Bulldogs

AP (Apr. 22, 2014) Drake University hosts 35th annual Beautiful Bulldog Contest. (April 21) Video provided by AP
Powered by NewsLook.com
805-Pound Shark Caught Off The Coast Of Florida

805-Pound Shark Caught Off The Coast Of Florida

Newsy (Apr. 22, 2014) One Florida fisherman caught a 805-pound shark off the coast of Florida earlier this month. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins