Featured Research

from universities, journals, and other organizations

New method for infectious diseases research

Date:
March 3, 2011
Source:
UmeŚ universitet
Summary:
Infectious diseases researchers are studying the surface properties of bacteria together with materials scientists. Studies of the outermost parts of the cell walls of bacteria yield new information about the chemical composition of structures that are important for the capacity of bacteria to infect organisms.

Infectious diseases researchers at UmeŚ University in Sweden are studying the surface properties of bacteria together with materials scientists. Studies of the outermost parts of the cell walls of bacteria yield new information about the chemical composition of structures that are important for the capacity of bacteria to infect organisms. The findings are now being reported in the Journal of Biological Chemistry.

Related Articles


When bacteria infect a host organism, they usually attach to tissue cells. Infectious diseases scientists at UmeŚ University are studying structural details of the outermost layer of bacterial cells in order to find new substances that can prevent bacterial infections. In collaboration with materials researchers at the Department of Chemistry, they describe new methods that facilitate and speed up their studies.

Chemist Madeleine Ramstedt is pursuing research on a material with new properties that prevent bacteria from attaching to its surface. The new material would be optimal for equipment in health care, where biofilms of bacteria can be a source of infection. In her research, Madeleine Ramstedt uses spectroscopic methods, among others, that she is now making available to her colleagues in the research consortium UmeŚ Centre for Microbial Research, UCMR.

Microbiologists Sun Nyunt Wai, Ryoma Nakao, and Bernt Eric Uhlin, together with chemists Jean-FranÁois Boily and Madeleine Ramstedt, were investigating whether new physiochemical analysis methods could also be used for microbial studies. The scientists combined so-called cryo-x-ray photoelectron spectroscopy with multivariate analysis. This analysis yields specific patterns of intensity curves depending on the chemical composition of the surface of the material being studied.

"We've succeeded also in analyzing the cell surfaces of bacteria with our x-ray spectroscopy. We found strong patterns that we could clearly relate to different compositions in lipids, sugar, protein, and the polymer peptidoglycan in the cell wall of the bacterium that can affect the capacity of a bacterium to infect an organism," explains Madeleine Ramstedt. "The method makes it possible to analyze the outermost layer, about 10 nanometers from the surface."

"Our method is relatively simple in comparison with other methods in which the extraction of various cell components is needed. This means that with our method the surface of the bacteria can be examined under more natural conditions in an intact bacterial cell."

X-ray photoelectron spectroscopy has previously been used to study bacteria, but only to a limited extent. The UmeŚ scientists have managed to optimize the method. "We shock freeze the bacteria and keep them frozen throughout the analysis. This allows us to assume that they do not change during the examination. Now it's possible to compare the cell walls in similar bacteria that have been treated in different ways or that have changed, for example by developing resistance. With our method we can now compare structures in cell walls in pathogenic bacteria with those of non-pathogenic bacteria, all on a larger scale. Hopefully this new method of analysis will yield more rapid results and provide infectious diseases researchers with new clues for finding new antibiotics," says Madeleine Ramstedt.

UCMR is one of UmeŚ University's strong research environments. The centre is an interdisciplinary research consortium that brings together a number of research teams in microbial research with participation from chemistry, medical and clinical microbiology, molecular biology, physics, and bioinformatics.


Story Source:

The above story is based on materials provided by UmeŚ universitet. Note: Materials may be edited for content and length.


Journal Reference:

  1. M. Ramstedt, R. Nakao, S. N. Wai, B. E. Uhlin, J.-F. Boily. Monitoring surface chemistry changes in the bacterial cell wall - multivariate analysis of Cryo-X-ray photoelectron spectroscopy data. Journal of Biological Chemistry, 2011; DOI: 10.1074/jbc.M110.209536

Cite This Page:

UmeŚ universitet. "New method for infectious diseases research." ScienceDaily. ScienceDaily, 3 March 2011. <www.sciencedaily.com/releases/2011/03/110303065339.htm>.
UmeŚ universitet. (2011, March 3). New method for infectious diseases research. ScienceDaily. Retrieved November 29, 2014 from www.sciencedaily.com/releases/2011/03/110303065339.htm
UmeŚ universitet. "New method for infectious diseases research." ScienceDaily. www.sciencedaily.com/releases/2011/03/110303065339.htm (accessed November 29, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Saturday, November 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Research on Bats Could Help Develop Drugs Against Ebola

Research on Bats Could Help Develop Drugs Against Ebola

AFP (Nov. 28, 2014) ó In Africa's only biosafety level 4 laboratory, scientists have been carrying out experiments on bats to understand how virus like Ebola are being transmitted, and how some of them resist to it. Duration: 01:18 Video provided by AFP
Powered by NewsLook.com
New Dinosaur Species Found in Museum Collection

New Dinosaur Species Found in Museum Collection

Reuters - Innovations Video Online (Nov. 27, 2014) ó A British palaeontologist has discovered a new species of dinosaur while studying fossils in a Canadian museum. Pentaceratops aquilonius was related to Triceratops and lived at the end of the Cretaceous Period, around 75 million years ago. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Tryptophan Isn't Making You Sleepy On Thanksgiving

Tryptophan Isn't Making You Sleepy On Thanksgiving

Newsy (Nov. 27, 2014) ó Tryptophan, a chemical found naturally in turkey meat, gets blamed for sleepiness after Thanksgiving meals. But science points to other culprits. Video provided by Newsy
Powered by NewsLook.com
Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) ó The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:††

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:††

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile:† iPhone Android Web
Follow:† Facebook Twitter Google+
Subscribe:† RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins