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Key metabolic pathway implicated in intractable form of breast cancer

Date:
July 19, 2011
Source:
Whitehead Institute for Biomedical Research
Summary:
Using a new in vivo screening system, researchers have identified a protein in a key metabolic pathway that is essential in estrogen receptor (ER)-negative breast cancer. When the expression of the gene that codes for this protein -- phosphoglycerate dehydrogenase or PHGDH -- is suppressed in tumors and cell lines with an overabundance of the protein, the rate of cellular growth declines markedly. PHGDH is overexpressed in approximately 70 percent of ER-negative breast cancer patients.

Using a new in vivo screening system, Whitehead Institute researchers have identified a protein in the serine biosynthesis pathway that is essential in estrogen receptor (ER)-negative breast cancer -- a notoriously difficult disease to treat associated with low five-year survival rates.

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According to the researchers, when expression of the gene that codes for this protein -- phosphoglycerate dehydrogenase or PHGDH -- is suppressed in tumors and cell lines with an overabundance of the protein, the rate of cellular growth declines markedly.

As reported this month in Nature, the in vivo screen focused on 133 metabolic genes that the researchers predicted to be necessary for tumorigenesis. Using RNA interference (RNAi), first author Richard Possemato targeted these genes in human breast cancer cells implanted in mice.

"Our goal for this study was to look for essential cancer genes in vivo, where the levels of metabolites are likely more appropriate than in an in vitro model system," says Possemato, a postdoctoral researcher in the lab of Whitehead Member David Sabatini.

In vivo screening provides a more realistic understanding of what would work in a living organism rather than in a Petri dish's artificial environment. During the screen Possemato and colleagues identified PHGDH, which is overexpressed in approximately 70% of ER-negative breast cancer patients, as essential to tumor growth. The PHGDH protein is one of three enzymes involved in the metabolic serine biosynthesis pathway. Cancer cells alter their metabolism in the interest of sustaining rapid growth, and high levels of PHGDH appear to drive such metabolic change. When Possemato suppressed PHGDH protein production in breast cancer cell lines with elevated levels of it, the cells stopped proliferating.

The findings suggest that PHGDH may represent a promising target for drug development for ER-negative breast cancer.

"We do think this has some therapeutic relevance, where an inhibitors of this enzyme would have effects on the cells we identified that tend to overexpress this enzyme," says Sabatini, who is also a biology professor at MIT. "By RNAi, we've provided proof of principle, but whether a drug against this protein would be valuable remains to be determined."

This research was supported by Susan G. Komen for the Cure, Life Science Research Foundation, Keck Foundation, David H. Koch Institute for Integrative Cancer Research at MIT, The Alexander and Margaret Stewart Trust Fund, and National Institutes of Health (NIH).

Sabatini serves as a Member of the Scientific Advisory Board of Agios Pharmaceuticals.


Story Source:

The above story is based on materials provided by Whitehead Institute for Biomedical Research. The original article was written by Nicole Giese. Note: Materials may be edited for content and length.


Journal Reference:

  1. Richard Possemato, Kevin M. Marks, Yoav D. Shaul, Michael E. Pacold, Dohoon Kim, Kıvanη Birsoy, Shalini Sethumadhavan, Hin-Koon Woo, Hyun G. Jang, Abhishek K. Jha, Walter W. Chen, Francesca G. Barrett, Nicolas Stransky, Zhi-Yang Tsun, Glenn S. Cowley, Jordi Barretina, Nada Y. Kalaany, Peggy P. Hsu, Kathleen Ottina, Albert M. Chan, Bingbing Yuan, Levi A. Garraway, David E. Root, Mari Mino-Kenudson, Elena F. Brachtel, Edward M. Driggers, David M. Sabatini. Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature, 2011; DOI: 10.1038/nature10350

Cite This Page:

Whitehead Institute for Biomedical Research. "Key metabolic pathway implicated in intractable form of breast cancer." ScienceDaily. ScienceDaily, 19 July 2011. <www.sciencedaily.com/releases/2011/07/110718164209.htm>.
Whitehead Institute for Biomedical Research. (2011, July 19). Key metabolic pathway implicated in intractable form of breast cancer. ScienceDaily. Retrieved October 24, 2014 from www.sciencedaily.com/releases/2011/07/110718164209.htm
Whitehead Institute for Biomedical Research. "Key metabolic pathway implicated in intractable form of breast cancer." ScienceDaily. www.sciencedaily.com/releases/2011/07/110718164209.htm (accessed October 24, 2014).

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