Featured Research

from universities, journals, and other organizations

The newest of the new in gene therapy: 'Tag and target and exchange'

Date:
December 14, 2011
Source:
Federation of American Societies for Experimental Biology
Summary:
New research demonstrates how a combination of two techniques improves the efficiency of experimental gene therapies, while reducing side effects. Scientists combined techniques involving site-specific recombinases that facilitate the exchange of genetic material between DNA strands, to guide where new genetic material is inserted into a cell's DNA. This approach to gene therapy represents an important advance, and has the potential to correct root causes of numerous illnesses.

A combination of two techniques promises to improve the efficiency and effectiveness of experimental gene therapies, while also reducing potential side effects says a new research report published in the December 2011 issue of the FASEB Journal. The report describes how scientists from Germany combined two techniques involving the use of site-specific recombinases, or enzymes that facilitate the exchange of genetic material between DNA strands, to help guide exactly where new genetic material is inserted into a cell's DNA. This experimental approach to gene therapy represents an important advance, as successful gene therapy has the potential to correct the root cause of numerous illnesses and health conditions.

"The central outcome of these and related techniques is the predictability and safety of a therapeutic regimen," said Juergen Bode, a researcher involved in the work from the Institute of Experimental Hematology at Hanover Medical School in Hanover, Germany. "These novel strategies will obviate the majority of animal experiments that are presently needed; it will enhance the effectiveness and shorten the timeline."

To make this discovery, Bode and colleagues identified two types of site-specific recombinases (SSR), one from yeast (Flp recombinase) and one from phages (PhiC31 recombinase), which are capable of tagging and targeting specific areas in a DNA strand. Specifically, the tagging process involves mounting a distinct address within a genome, whereas the targeting process covers the delivery of genetic material to this address. PhiC31was identified as an ideal enzyme for tagging because it recognizes just a limited number of pre-existent genomic addresses with well-known and mostly beneficial characteristics, allows for only a one-way transfer of genetic material, and is basically irreversible. In contrast, Flp recombinase acts in a reversible manner, meaning that a given target can be modified over and over again to study the role of slight changes in the structure of therapeutic proteins. Although both techniques have particular properties, they complement one another and may even be used in conjunction to obtain ultimate results.

"Successful gene therapy has been called the Holy Grail of biomedical research. It could revolutionize medicine as much, if not more, than the development of antibiotics," said Gerald Weissmann, M.D., Editor-in-Chief of the FASEB Journal. "While we have a long way to go, this report is an important advance. It shows us how to insert important genetic material exactly where it is needed, rather than blindly popping it into a cell and hoping for the best."


Story Source:

The above story is based on materials provided by Federation of American Societies for Experimental Biology. Note: Materials may be edited for content and length.


Journal Reference:

  1. S. Turan, J. Bode. Site-specific recombinases: from tag-and-target- to tag-and-exchange-based genomic modifications. The FASEB Journal, 2011; 25 (12): 4088 DOI: 10.1096/fj.11-186940

Cite This Page:

Federation of American Societies for Experimental Biology. "The newest of the new in gene therapy: 'Tag and target and exchange'." ScienceDaily. ScienceDaily, 14 December 2011. <www.sciencedaily.com/releases/2011/11/111130115822.htm>.
Federation of American Societies for Experimental Biology. (2011, December 14). The newest of the new in gene therapy: 'Tag and target and exchange'. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2011/11/111130115822.htm
Federation of American Societies for Experimental Biology. "The newest of the new in gene therapy: 'Tag and target and exchange'." ScienceDaily. www.sciencedaily.com/releases/2011/11/111130115822.htm (accessed July 31, 2014).

Share This




More Health & Medicine News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Xtreme Eating: Your Daily Caloric Intake All On One Plate

Xtreme Eating: Your Daily Caloric Intake All On One Plate

Newsy (July 30, 2014) The Center for Science in the Public Interest released its 2014 list of single meals with whopping calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



    Save/Print:
    Share:

    Free Subscriptions


    Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

    Get Social & Mobile


    Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

    Have Feedback?


    Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
    Mobile: iPhone Android Web
    Follow: Facebook Twitter Google+
    Subscribe: RSS Feeds Email Newsletters
    Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins