Featured Research

from universities, journals, and other organizations

Blu-ray player detects microorganisms and toxins on discs

Date:
February 18, 2014
Source:
Plataforma SINC
Summary:
In addition to storing films, optical discs can be used to detect microorganisms, toxins, allergens and tumoral biomarkers. Blu-ray technology has allowed researchers to develop a way to find out if a sample contains Salmonella or toxic substances. This simple and cheap analytical system may be applied to clinical diagnosis and environmental monitoring.

Samples deposited on the disc. In each drop there are 64 points (8 x 8), each 125 µm in diameter.
Credit: UPV

In addition to storing films, optical discs can be used to detect microorganisms, toxins, allergens and tumoral biomarkers. Blu-ray technology has allowed researchers at the Polytechnic University of Valencia to develop a way to find out if a sample contains Salmonella or toxic substances. This simple and cheap analytical system may be applied to clinical diagnosis and environmental monitoring.

A system devised by Chemists at the Polytechnic University of Valencia (UPV, Spain) uses Blu-ray discs and players to detect pathogenic bacteria and toxins in biological samples. The journal 'Biosensors and Bioelectronics' published the study.

"We use the surface of these commercial discs as a platform for analysis to carry out the tests and then, with the laser reader of the recorder/player we can identify the bacteria and determine their concentration," Sergi Morais, one of the authors, explained.

Specifically, the team has analysed the DNA of two types of pathogenic bacteria in this way: Salmonella typhimurium, which causes salmonellosis, and Cronobacter sakazakii, an intestinal parasite. "The method could be used to detect these and other microorganisms in breast milk or other foods," says Morais.

The researchers also applied Blu-ray technology to determine the concentration of microcystins in water, a kind of toxin that causes gastrointestinal disorders or allergic reactions, and which are produced by cyanobacteria.

"This type of test can also be used to detect tumoral biomarkers, food and drug allergens, and pesticides in water, for example," the researcher states. "The level of pollutants in water and air are regulated by directives that set a maximum residue limit, and the sensitivity of our technique allows analytes below that required by government to be detected."

The samples are deposited in small quantities on the discs in the form of microarrays or two-dimensional arrays. "The hydrophobic nature of the surface of the Blu-ray disc allows the proteins to be locked in place by passive adsorption in a high-density format (64 points in each 1 mm2 drop)," one of the authors, Ángel Maquieira, points out.

"On the 90 cm2 surface of these discs it's possible to imprint 138,000 points, each one 125 µm in diameter," he adds. "Furthermore, the low sample volume used (5-10 microlitres) and the low cost of the developed hardware make this technology a very practical and economically competitive tool."

According to the researchers, the accuracy and sensitivity of these electronic devices is similar to that obtained with conventional laboratory techniques, such as the quantitative polymerase chain reaction (PCR). Though it's not an "official" methodology, the process offers a very practical strategy to eliminate samples before applying more exhaustive analytical techniques. "Samples that are shown to be positive by this methodology will also be positive using the other techniques," Morais explains.

Its characteristics make Blu-ray technology a "promising alternative" due the fact it may be applied to the development of new systems of analysis used in clinical diagnosis, or be used in situ, in the field, in the agrofood industry, and in environmental monitoring.

The study warns that the lack of simple sensor systems that combine a high detection resolution with low costs is the reason why some analytical technologies are not found in installations with a low budget.


Story Source:

The above story is based on materials provided by Plataforma SINC. Note: Materials may be edited for content and length.


Journal Reference:

  1. Tania Arnandis-Chover, Sergi Morais, Miguel Ángel González-Martínez, Rosa Puchades, Ángel Maquieira. High density MicroArrays on Blu-ray discs for massive screening. Biosensors and Bioelectronics, 2014; 51: 109 DOI: 10.1016/j.bios.2013.07.045

Cite This Page:

Plataforma SINC. "Blu-ray player detects microorganisms and toxins on discs." ScienceDaily. ScienceDaily, 18 February 2014. <www.sciencedaily.com/releases/2014/02/140218110529.htm>.
Plataforma SINC. (2014, February 18). Blu-ray player detects microorganisms and toxins on discs. ScienceDaily. Retrieved October 23, 2014 from www.sciencedaily.com/releases/2014/02/140218110529.htm
Plataforma SINC. "Blu-ray player detects microorganisms and toxins on discs." ScienceDaily. www.sciencedaily.com/releases/2014/02/140218110529.htm (accessed October 23, 2014).

Share This



More Plants & Animals News

Thursday, October 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Working Mother DIY: Pumpkin Pom-Pom

Working Mother DIY: Pumpkin Pom-Pom

Working Mother (Oct. 22, 2014) — How to make a pumpkin pom-pom. Video provided by Working Mother
Powered by NewsLook.com
Goofy Dinosaur Blends Barney and Jar Jar Binks

Goofy Dinosaur Blends Barney and Jar Jar Binks

AP (Oct. 22, 2014) — A collection of dinosaur bones reveal a creature that is far more weird and goofy-looking than scientists originally thought when they found just the arm bones nearly 50 years ago, according to a new report in the journal Nature. (Oct. 22) Video provided by AP
Powered by NewsLook.com
San Diego Zoo's White Rhinos Provide Hope for the Critically Endangered Species

San Diego Zoo's White Rhinos Provide Hope for the Critically Endangered Species

Reuters - Light News Video Online (Oct. 22, 2014) — The pair of rare white northern rhinos bring hope for their species as only six remain in the world. Elly Park reports. Video provided by Reuters
Powered by NewsLook.com
Raw: Bear Cub Strolls Through Oregon Drug Store

Raw: Bear Cub Strolls Through Oregon Drug Store

AP (Oct. 22, 2014) — Shoppers at an Oregon drug store were surprised by a bear cub scurrying down the aisles this past weekend. (Oct. 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins