Featured Research

from universities, journals, and other organizations

'MaMTH' advance: New technology sheds light on protein interactions

Date:
March 24, 2014
Source:
University of Toronto
Summary:
Scientists have a better way to study human proteins -- large molecules that are part of every cell in the body -- thanks to a new technology. The technology tracks a class of proteins called membrane proteins as they interact with other proteins to either maintain health or contribute to disease.

“This technology gives us a new tool to examine membrane proteins in their natural environment of the human cell,” said Igor Stagljar, a Professor in the Donnelly Centre for Cellular and Biomolecular Research.
Credit: Image courtesy of University of Toronto

Scientists have a better way to study human proteins -- large molecules that are part of every cell in the body -- thanks to a new technology developed by University of Toronto researchers. The technology tracks a class of proteins called membrane proteins as they interact with other proteins to either maintain health or contribute to disease.

Related Articles


Membrane proteins make up about one third of all proteins in the human body, and their malfunction is associated with more than 500 diseases. But they've been hard to study because understanding their role depends on observing their interactions with other proteins.

"This technology gives us a new tool to examine membrane proteins in their natural environment of the human cell," said Igor Stagljar, a Professor in the Donnelly Centre for Cellular and Biomolecular Research. "As well, it's sensitive enough to detect minor changes upon introduction of drugs, so it should prove useful in the development of therapeutics, particularly for cancer and neurological diseases."

The journal Nature Methods published the research online today.

Stagljar and his colleagues also applied the new technology, which they dubbed MaMTH (for Mammalian-Membrane Two-Hybrid assay), to identify a protein that plays a role in the most common form of lung cancer called non-small cell lung cancer.

That protein, CRKII, interacts with another protein called an epidermal growth factor receptor. Mutation of this receptor -- which is already the target of several cancer drugs either approved or in development -- causes a proliferation of cancer cells.

"CRKII most likely regulates the stability of mutated epidermal growth factor receptors and drives cancer growth by promoting signaling, or communication, within cancer cells," said Julia Petschnigg, lead author on the paper and a postdoctoral fellow at U of T. "We found that a combinatorial chemotherapy that inhibits those mutated receptors and CRKII could be beneficial in treating lung cancer."

The research was highly collaborative, involving cancer clinicians, bioinformaticians and researchers in five labs around Toronto and Boston. Stagljar and his lab spent four years developing the MaMTH technology, which they adapted from a similar technology that captures protein-protein interactions in yeast.

Next, the group will apply their technology to study some of the 500 proteins that are mutated in other human diseases.

"You simply cannot publish meaningful research in proteomics without collaborating," said Stagljar, who is also a Professor in the Departments of Biochemistry and Molecular Genetics. "Fortunately, we have access to great cross-disciplinary expertise and infrastructure in the Donnelly Centre. Science is alive and well in Toronto."


Story Source:

The above story is based on materials provided by University of Toronto. Note: Materials may be edited for content and length.


Journal Reference:

  1. Julia Petschnigg, Bella Groisman, Max Kotlyar, Mikko Taipale, Yong Zheng, Christoph F Kurat, Azin Sayad, J Rafael Sierra, Mojca Mattiazzi Usaj, Jamie Snider, Alex Nachman, Irina Krykbaeva, Ming-Sound Tsao, Jason Moffat, Tony Pawson, Susan Lindquist, Igor Jurisica, Igor Stagljar. The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nature Methods, 2014; DOI: 10.1038/nmeth.2895

Cite This Page:

University of Toronto. "'MaMTH' advance: New technology sheds light on protein interactions." ScienceDaily. ScienceDaily, 24 March 2014. <www.sciencedaily.com/releases/2014/03/140324104600.htm>.
University of Toronto. (2014, March 24). 'MaMTH' advance: New technology sheds light on protein interactions. ScienceDaily. Retrieved December 20, 2014 from www.sciencedaily.com/releases/2014/03/140324104600.htm
University of Toronto. "'MaMTH' advance: New technology sheds light on protein interactions." ScienceDaily. www.sciencedaily.com/releases/2014/03/140324104600.htm (accessed December 20, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, December 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

The Best Tips to Curb Holiday Carbs

The Best Tips to Curb Holiday Carbs

Buzz60 (Dec. 19, 2014) It's hard to resist those delicious but fattening carbs we all crave during the winter months, but there are some ways to stay satisfied without consuming the extra calories. Vanessa Freeman (@VanessaFreeTV) has the details. Video provided by Buzz60
Powered by NewsLook.com
Sierra Leone Bikers Spread the Message to Fight Ebola

Sierra Leone Bikers Spread the Message to Fight Ebola

AFP (Dec. 19, 2014) More than 100 motorcyclists hit the road to spread awareness messages about Ebola. Nearly 7,000 people have now died from the virus, almost all of them in west Africa, according to the World Health Organization. Video provided by AFP
Powered by NewsLook.com
Researchers Test Colombian Village With High Alzheimer's Rates

Researchers Test Colombian Village With High Alzheimer's Rates

AFP (Dec. 19, 2014) In Yarumal, a village in N. Colombia, Alzheimer's has ravaged a disproportionately large number of families. A genetic "curse" that may pave the way for research on how to treat the disease that claims a new victim every four seconds. Duration: 02:42 Video provided by AFP
Powered by NewsLook.com
The Best Protein-Filled Foods to Energize You for the New Year

The Best Protein-Filled Foods to Energize You for the New Year

Buzz60 (Dec. 19, 2014) The new year is coming and nothing will energize you more for 2015 than protein-filled foods. Fitness and nutrition expert John Basedow (@JohnBasedow) gives his favorite high protein foods that will help you build muscle, lose fat and have endless energy. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins