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Researcher using next-generation sequencing, other new methods to rapidly identify pathogens

Date:
July 28, 2014
Source:
Kansas State University
Summary:
A recently published study looks at the discovery of porcine enterovirus G, which is an important find in the United States. The virus is thought to be benign and is not known to cause disease, but it had only been reported before in Europe and Asia. "We had isolated a virus in cells, but didn't know what it was," one researcher said. "We used next-generation sequencing to identify it, and it turned out to be porcine enterovirus G, which had been described before but had never previously been found in North America."

Benjamin Hause has joined the Kansas State Veterinary Diagnostic Laboratory as a research assistant professor. Hause uses next-generation sequencing and other new methods to rapidly identify pathogens.
Credit: Kansas State University

He calls himself the bug hunter, but the target of his work consists of viruses that can only be found and identified with special methods and instruments. Benjamin Hause, an assistant research professor at the Kansas State Veterinary Diagnostic Laboratory at Kansas State University, recently published an article about one of his discoveries, porcine enterovirus G, which is an important find in the United States.

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"We had isolated a virus in cells, but didn't know what it was," Hause said. "We used next-generation sequencing to identify it, and it turned out to be porcine enterovirus G, which had been described before but had never previously been found in North America."

The virus is thought to be benign and is not known to cause disease, but it had only been reported before in Europe and Asia.

"Fortunately porcine enterovirus G doesn't do much in pigs, but it raises concerns about other viruses getting through the border," Hause said. "We're not sure if this has been here for some time undetected or is a recent introduction. Coincidentally, the virus was most similar to 2012 Chinese isolates and was detected around the same time as a couple of other viruses: porcine epidemic diarrhea virus and porcine deltacoronavirus, both of which were detected in China in the same time frame prior to the U.S."

Both porcine epidemic diarrhea virus and porcine deltacoronavirus have caused major economic losses to hog producers in North America and are impacting several other aspects of the swine industry. Hause has mapped these viruses at the Kansas State Veterinary Diagnostic Laboratory as a way to ensure the reliability of the next-generation sequencing methods he uses to identify and characterize pathogens. The technique will soon be offered at the laboratory.

"As we isolate viruses, we can completely sequence their genomes," Hause said. "We can get a good understanding of what makes those viruses tick. Next-generation sequencing goes farther and allows us to perform metagenomic sequencing where we don't isolate the virus. Instead, we can sequence all DNA contained in a sample, which includes the host DNA, plus it reads all of the viruses in the sample too. It's a universal method to detect viruses that we have adapted and applied to veterinary diagnostics."

Hause's most recent work has led to the discovery of an influenza virus in cattle.

"A swine sample came in that we thought was influenza, but all other tests were negative," Hause said. "We found instead that this was an entirely new type of influenza. Subsequent research has shown that it is widespread in cattle, not just pigs. Now we're studying the association of this strain of bovine influenza with respiratory disease in feedlots."

Before joining Kansas State University in May, Hause was the diagnostic lab director at Newport Labs in Worthington, Minnesota. He worked on his porcine enterovirus G paper with Richard Hesse, a diagnostic virologist at the Kansas State Veterinary Diagnostic Laboratory, who helped recruit Hause to the university to further develop his next-generation sequencing methodology, which has other benefits from identifying and characterizing pathogens.

"As a virus mutates and changes, next-generation sequencing can be used to help update vaccines so they are still effective," Hause said. "Through this technology we can build a database with a collection of viruses based on where they came from and what kind of clinical presentation was seen. Then we can mine that dataset to match the vaccines or to get additional information on the pathogen. Some diseases such as flu mutate and change rapidly, and can jump from humans to pigs and back to humans, so it's important for both animal health and human health that we monitor and understand these viruses as much as possible."


Story Source:

The above story is based on materials provided by Kansas State University. The original article was written by Joe Montgomery. Note: Materials may be edited for content and length.


Journal Reference:

  1. Srivishnupriya Anbalagan, Richard A. Hesse, Ben M. Hause. First Identification and Characterization of Porcine Enterovirus G in the United States. PLoS ONE, 2014; 9 (5): e97517 DOI: 10.1371/journal.pone.0097517

Cite This Page:

Kansas State University. "Researcher using next-generation sequencing, other new methods to rapidly identify pathogens." ScienceDaily. ScienceDaily, 28 July 2014. <www.sciencedaily.com/releases/2014/07/140728113133.htm>.
Kansas State University. (2014, July 28). Researcher using next-generation sequencing, other new methods to rapidly identify pathogens. ScienceDaily. Retrieved October 24, 2014 from www.sciencedaily.com/releases/2014/07/140728113133.htm
Kansas State University. "Researcher using next-generation sequencing, other new methods to rapidly identify pathogens." ScienceDaily. www.sciencedaily.com/releases/2014/07/140728113133.htm (accessed October 24, 2014).

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