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Brooding brittle star babies in 3-D

A plethora of images from live-bearing brittle stars allows a 3-D look inside these unique starfish relatives and an insider's view of their soon-to-be offspring

Date:
November 17, 2015
Source:
GigaScience
Summary:
A new article presents 3-D images of the inside of live-bearing brittle stars, relatives of starfish. The high-resolution images allow the analysis of developing brittle star young, but also -- besides being cool to look at -- are perfect for sharing with other researchers. Large-data sharing, though difficult, is an essential part of making research both reproducible and reusable in the broadest sense.
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The 5.4 ?m isotropic 197 voxel size scan of a brittle star species, Ampiura capensis, reveals calcified skeleton components (intensely bright areas), the juveniles developing inside (note also indicator for juvenile disk and arm), and soft tissues such as the bursal wall.
Credit: The image is under a CC-BY license. From Landschoff J, Du Plessis A, Griffiths CL. 2015a. A dataset describing brooding in three species of South African brittle stars, comprising seven high-resolution, X-ray micro tomography scans. GigaScience 2015: 4. doi:10.1186/s13742-015-0093-2.

A plethora of images from live-bearing brittle stars allows a 3D look inside these unique starfish relatives and an insider's view of their soon-to-be offspring.

Published today in GigaScience is an article that describes high-resolution 3D images, data, and videos of five individuals from three different species of live-bearing brittle stars. The entire associated 100GB of data is freely available in the GigaScience database, GigaDB. These data, generated using non-invasive micro X-ray computed tomography (microCT), were used to study the development of young brooded inside the brittle star, but the value of these data -- besides being cool to look at -- is in their suitability for broad sharing for other researchers to use to examine juveniles within the adult as well as to carry out comparative morphological or anatomical analyses. Large-data sharing is an essential part of making research both reproducible and reusable in the broadest sense. Enabling better understanding of the complex interplay between internal structures can also help throw new insight into how species interact with their environments.

Brittle stars are a class of organisms that are closely related to starfish. While most members of this group reproduce externally, there are some species that develop their young internally, and then 'give birth' to live young. While sonograms are the means by which humans can get a look at their developing offspring, live-bearing brittle stars have not been so lucky, as researchers primary means to investigate the brooding chambers and juveniles of brittle stars has been via dissection. This technique destroys the sample, thus eliminating the possibility of further study and sharing of samples for others to use. Here, by using microCT scanning, the authors were able to visualize in 3D the brooding chambers (bursae) and juveniles inside the brittle star, providing an in situ view of the developing young and the position of the bursae inside the adult. Further, because the process doesn't damage the brittle star, additional analyses can be carried out on the same individual.

First author Jannes Landschoff from the Applied Marine Science Institute at the University of Cape Town states: "Our goal was to visualise the very large brooded juveniles inside the adults without disturbing the surrounding tissues. At first, we purely wanted to show the feasibility, but we soon realised how amazing the three-dimensional visualisations looked and that our rotation movies attracted much interest." Recognizing the extremely high quality of the images they used to study brittle star development, and that the data included much more information than they actually used, they made all these data available to others in a fully open-access format through GigaScience's repository, GigaDB. The data includes videos and projection, reconstruction images and an additional volume file for easy viewing.


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Journal References:

  1. Jannes Landschoff, Anton Du Plessis, Charles L. Griffiths. A dataset describing brooding in three species of South African brittle stars, comprising seven high-resolution, micro X-ray computed tomography scans. GigaScience, 2015; 4 (1) DOI: 10.1186/s13742-015-0093-2
  2. Du Plessis, A; Landschoff, J; Griffiths, C, L. 3-dimensional microCT reconstructions of brooding brittle stars. GigaScience Database, 2015 DOI: 10.5524/100130
  3. J Landschoff, CL Griffiths. Three-dimensional visualisation of brooding behaviour in two distantly related brittle stars from South African waters. African Journal of Marine Science, 2015; 1 DOI: 10.2989/1814232X.2015.1095801

Cite This Page:

GigaScience. "Brooding brittle star babies in 3-D: A plethora of images from live-bearing brittle stars allows a 3-D look inside these unique starfish relatives and an insider's view of their soon-to-be offspring." ScienceDaily. ScienceDaily, 17 November 2015. <www.sciencedaily.com/releases/2015/11/151117091225.htm>.
GigaScience. (2015, November 17). Brooding brittle star babies in 3-D: A plethora of images from live-bearing brittle stars allows a 3-D look inside these unique starfish relatives and an insider's view of their soon-to-be offspring. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2015/11/151117091225.htm
GigaScience. "Brooding brittle star babies in 3-D: A plethora of images from live-bearing brittle stars allows a 3-D look inside these unique starfish relatives and an insider's view of their soon-to-be offspring." ScienceDaily. www.sciencedaily.com/releases/2015/11/151117091225.htm (accessed May 26, 2017).

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