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Harnessing CRISPR for rapid detection of viral and bacterial infection

Date:
April 13, 2017
Source:
American Association for the Advancement of Science
Summary:
Researchers have created a version of CRISPR-Cas that can be used to diagnose infections, such as Zika and dengue, with a high level of sensitivity.
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Researchers have created a version of CRISPR-Cas that can be used to diagnose infections, such as Zika and dengue, with a high level of sensitivity.

The advancement could help facilitate rapid detection and diagnosis of many other pathogens, too. While some methods exist for detecting genetic sequences, they have trade-offs among sensitivity, specificity, simplicity, cost, and speed. In the search for a more effective method, Feng Zhang, Jonathan S. Gootenberg and colleagues turned to a CRISPR-Cas system that targets RNA.

Binding the target RNA activates this particular Cas enzyme to promiscuously cleave nearby RNA. They exploited this by including a reporter RNA that releases a fluorescent signal when it is cleaved, and used a technique, called recombinase polymerase amplification, to further boost sensitivity.

This combination resulted in a system, which the team dubbed SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing), that could detect rather low titers of Zika virus in serum, urine, and saliva. SHERLOCK can also be used to measure viral load, the authors show.

Next, they found that the technique was effective at identifying various bacterial strains, even differentiating between strains of bacterial pneumonia with different resistance mutations. Lastly, the authors show that SHERLOCK can be used to detect different cancer mutations.

A SHERLOCK test can be redesigned and synthesized in a matter of days for as low as $0.61/test, the authors say, noting that the high sensitivity of the system opens new avenues for rapid, robust and sensitive detection of biological molecules.


Story Source:

Materials provided by American Association for the Advancement of Science. Note: Content may be edited for style and length.


Journal Reference:

  1. Jonathan S. Gootenberg et al. Nucleic acid detection with CRISPR-Cas13a/C2c2. Science, 2017 DOI: 10.1126/science.aam9321

Cite This Page:

American Association for the Advancement of Science. "Harnessing CRISPR for rapid detection of viral and bacterial infection." ScienceDaily. ScienceDaily, 13 April 2017. <www.sciencedaily.com/releases/2017/04/170413141106.htm>.
American Association for the Advancement of Science. (2017, April 13). Harnessing CRISPR for rapid detection of viral and bacterial infection. ScienceDaily. Retrieved May 27, 2017 from www.sciencedaily.com/releases/2017/04/170413141106.htm
American Association for the Advancement of Science. "Harnessing CRISPR for rapid detection of viral and bacterial infection." ScienceDaily. www.sciencedaily.com/releases/2017/04/170413141106.htm (accessed May 27, 2017).

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