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New sensor gives unprecedented look at changes in cell's energy 'currency'

Date:
May 16, 2024
Source:
Howard Hughes Medical Institute
Summary:
A new sensor is giving researchers the best look yet at ATP levels inside living cells, enabling scientists to study in greater detail than ever before how fluctuations in this cellular currency affect the cell and contribute to disease.
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FULL STORY

Just as the US economy runs on the dollar, the cellular economy runs on ATP. The energy-carrying molecule fuels nearly all processes inside the cell, making ATP critical for cellular life.

Now, a new sensor developed at Janelia is giving researchers the best look yet at ATP levels inside living cells, enabling scientists to study in greater detail than ever before how fluctuations in this cellular currency affect the cell and contribute to disease.

Although ATP is critically important to cells, there haven't been good ways for scientists to track how it changes in living cells. Previous ATP sensors were dim, slow, or difficult to use.

In 2019, Janelia and UCLA researchers developed a fluorescent protein sensor, iATPSnFR, that works in a similar way to the popular GCaMP sensors used to detect calcium. A fluorescent molecule is attached to a protein that binds ATP. When this binding occurs, the protein changes shape, causing the fluorescent molecule to light up. This first-generation sensor could detect changes in ATP, but it only operated in a narrow range, so it was not useful for tracking changes in ATP concentrations inside cells.

Now, Janelia scientists and collaborators, led by Jonathan Marvin, a senior scientist on Janelia's Tool Translation Team, have developed the next generation sensor, iATPSnFR2, that can track ATP concentrations over a much larger range. This enables scientists to measure ATP inside living cells in much greater detail than ever before.

Tim Ryan, a researcher at Weill Cornell Medicine and a Janelia Scholar, who worked with Marvin and the team to develop and test the new sensor, used iATPSnFR2 to track changes in ATP at individual synapses, the junctions where neurons communicate. Ryan and his team are studying how changes in ATP activity at synapses could play a role in the development of Parkinson's disease. The new sensor enables them to directly observe these changes and home in on how these fluctuations could be contributing to the disease.

Beyond this research, the team expects the new sensor will be used by other scientists to study a wide range of research questions involving ATP that have been difficult to answer with previous tools.


Story Source:

Materials provided by Howard Hughes Medical Institute. Note: Content may be edited for style and length.


Journal Reference:

  1. Jonathan S. Marvin, Alexandros C. Kokotos, Mukesh Kumar, Camila Pulido, Ariana N. Tkachuk, Jocelyn Shuxin Yao, Timothy A. Brown, Timothy A. Ryan. iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP. Proceedings of the National Academy of Sciences, 2024; 121 (21) DOI: 10.1073/pnas.2314604121

Cite This Page:

Howard Hughes Medical Institute. "New sensor gives unprecedented look at changes in cell's energy 'currency'." ScienceDaily. ScienceDaily, 16 May 2024. <www.sciencedaily.com/releases/2024/05/240516160527.htm>.
Howard Hughes Medical Institute. (2024, May 16). New sensor gives unprecedented look at changes in cell's energy 'currency'. ScienceDaily. Retrieved June 19, 2024 from www.sciencedaily.com/releases/2024/05/240516160527.htm
Howard Hughes Medical Institute. "New sensor gives unprecedented look at changes in cell's energy 'currency'." ScienceDaily. www.sciencedaily.com/releases/2024/05/240516160527.htm (accessed June 19, 2024).

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