Featured Research

from universities, journals, and other organizations

New Method Used To Transfer Genes Into Mouse

Date:
April 2, 2002
Source:
University Of Minnesota
Summary:
For the first time, a special segment of DNA called a transposon and an enzyme known as the Sleeping Beauty transposase have been used to genetically modify a vertebrate animal. In the study, which is published in the April 2 issue of the Proceedings of the National Academy of Sciences, University of Minnesota researchers injected a transposon containing the gene for a yellow coat color into a mouse embryo, resulting in a genetically modified mouse.

MINNEAPOLIS / ST. PAUL (April 1, 2002)-- For the first time, a special segment of DNA called a transposon and an enzyme known as the Sleeping Beauty transposase have been used to genetically modify a vertebrate animal. In the study, which is published in the April 2 issue of the Proceedings of the National Academy of Sciences, University of Minnesota researchers injected a transposon containing the gene for a yellow coat color into a mouse embryo, resulting in a genetically modified mouse.

"This is a new type of technology, an entirely different way to make genetically modified animals," said David Largaespada, Ph.D., an assistant professor in the university's department of genetics, cell biology and development and director of the University of Minnesota Cancer Center's Genetic Mechanisms of Cancer research program. "The Sleeping Beauty transposase enzyme plus the transposon is like a truck used to carry the cargo, or specific genes, into the animal. These specific genes could help treat diseases such as cancer."

Researchers at the Cancer Center injected a one-cell mouse embryo--a fertilized egg--with a linear piece of DNA containing the transposon, along with a source of Sleeping Beauty transposase enzyme. In this case, the transposon contained the gene for making a yellow-colored coat. The enzyme then caused the transposon to "jump" from the linear piece of DNA to a mouse chromosome, where it was able to express its function of coat color.

"We're very excited about Sleeping Beauty’s potential," said Largaespada. "One use would be to add genes to germ cells or early embryos in order to produce large amounts of a protein in an animal. The protein then would be purified and used as a drug treatment for hemophilia, for instance."

Another function would be to create genetically altered farm animals, which could be used as a source of organs for transplantation. Gene identification and function may also be determined by transposons' ability to mutate or "knock out" genes.

"We and other scientists at the university, such as Scott McIvor, are also working on transferring genes directly into cells of the body, in the liver or lungs, for instance," said Largaespada. "We hope this procedure could help cure diseases such as cystic fibrosis or hemophilia."

Largaespada is co-founder of Minneapolis-based Discovery Genomics, Inc., which has exclusive license to Sleeping Beauty and plans to commercialize this technology. http://www.discoverygenomics.net/

The University of Minnesota Cancer Center is a National Cancer Institute-designated Comprehensive Cancer Center. Awarded more than $68 million in peer-reviewed grants during fiscal year 2001, the center conducts cancer research that advances knowledge and enhances care. The center also sponsors community outreach and public education efforts addressing cancer. For more information on cancer in general, visit the Web site at http://www.cancer.umn.edu or call (1 888-CANCERMN, 1-888-226-2376 or 612-624-2620).


Story Source:

The above story is based on materials provided by University Of Minnesota. Note: Materials may be edited for content and length.


Cite This Page:

University Of Minnesota. "New Method Used To Transfer Genes Into Mouse." ScienceDaily. ScienceDaily, 2 April 2002. <www.sciencedaily.com/releases/2002/04/020402072401.htm>.
University Of Minnesota. (2002, April 2). New Method Used To Transfer Genes Into Mouse. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/2002/04/020402072401.htm
University Of Minnesota. "New Method Used To Transfer Genes Into Mouse." ScienceDaily. www.sciencedaily.com/releases/2002/04/020402072401.htm (accessed July 30, 2014).

Share This




More Health & Medicine News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Generics Eat Into Pfizer's Sales

Generics Eat Into Pfizer's Sales

Reuters - Business Video Online (July 29, 2014) Pfizer, the world's largest drug maker, cut full-year revenue forecasts because generics could cut into sales of its anti-arthritis drug, Celebrex. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins