Featured Research

from universities, journals, and other organizations

'Kissing' RNA And HIV-1: Unraveling The Details

Date:
February 3, 2004
Source:
National Institute Of Standards And Technology (NIST)
Summary:
A subtle structural change that may play a role in the molecular machinery for making HIV-1 (the virus that causes AIDS) has been identified by scientists from the National Institute of Standards and Technology (NIST) and University of Maryland working at the Center for Advanced Research in Biotechnology (CARB).

A subtle structural change that may play a role in the molecular machinery for making HIV-1 (the virus that causes AIDS) has been identified by scientists from the National Institute of Standards and Technology (NIST) and University of Maryland working at the Center for Advanced Research in Biotechnology (CARB). If confirmed in living cells, the mechanism, described in the Jan. 20 online edition of Proceedings of the National Academy of Sciences, might provide a new target for antiviral drugs.

The finding is among several to emerge recently from CARB's efforts to develop and validate sensitive tools for rapidly detecting and quantifying ribonucleic acid (RNA) interactions. RNA provides the genetic blueprint for retroviruses such as HIV-1. CARB scientists created a model system for tracking changes in an RNA structural element involved in forming HIV-1 viral particles.

As new HIV-1 viruses form and mature into infectious particles, two strands of RNA interact through a transient structure called a "molecular kiss." This structure is then packaged into new virus particles, which undergo further maturation after release from the infected cell. CARB scientists found that specific sites in the "kissing" structure acquire a proton (a positively charged particle) at a pH close to that of living cells. The proton's presence alters the RNA structure and accelerates its refolding by a protein associated with viral maturation. Taken together, these observations suggest that such a mechanism might be at work during viral infection.

To track RNA binding and folding in real-time, the CARB scientists developed fluorescent markers as substitutes for pieces of the RNA. By monitoring changes in the fluorescent signal, the scientists could follow these reactions. The scientists also used nuclear magnetic resonance to identify the sites in the RNA that acquire the proton and to characterize the resulting conformational changes.


Story Source:

The above story is based on materials provided by National Institute Of Standards And Technology (NIST). Note: Materials may be edited for content and length.


Cite This Page:

National Institute Of Standards And Technology (NIST). "'Kissing' RNA And HIV-1: Unraveling The Details." ScienceDaily. ScienceDaily, 3 February 2004. <www.sciencedaily.com/releases/2004/02/040202070227.htm>.
National Institute Of Standards And Technology (NIST). (2004, February 3). 'Kissing' RNA And HIV-1: Unraveling The Details. ScienceDaily. Retrieved October 2, 2014 from www.sciencedaily.com/releases/2004/02/040202070227.htm
National Institute Of Standards And Technology (NIST). "'Kissing' RNA And HIV-1: Unraveling The Details." ScienceDaily. www.sciencedaily.com/releases/2004/02/040202070227.htm (accessed October 2, 2014).

Share This



More Health & Medicine News

Thursday, October 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Might Not Be Out Of Control In U.S., But Coverage Is

Ebola Might Not Be Out Of Control In U.S., But Coverage Is

Newsy (Oct. 2, 2014) Coverage of the lone Ebola patient discovered in Texas has U.S. media in a frenzy — but does the coverage match the reality? Video provided by Newsy
Powered by NewsLook.com
US Hunts Contacts of Ebola Patient, Including Children

US Hunts Contacts of Ebola Patient, Including Children

AFP (Oct. 2, 2014) Health officials in Texas on Wednesday scoured the Dallas area for people, including schoolchildren, who came in contact with a Liberian man who was diagnosed with Ebola in the United States. Duration: 00:55 Video provided by AFP
Powered by NewsLook.com
Study Says Losing Sense Of Smell Can Indicate Death

Study Says Losing Sense Of Smell Can Indicate Death

Newsy (Oct. 2, 2014) Researchers found elderly adults with a poor sense of smell are more likely to die within five years. Video provided by Newsy
Powered by NewsLook.com
Pregnancy Spacing Could Have Big Impact On Autism Risks

Pregnancy Spacing Could Have Big Impact On Autism Risks

Newsy (Oct. 1, 2014) A new study says children born less than one year and more than five years after a sibling can have an increased risk for autism. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins