ScienceDaily (Apr. 29, 2005) A small, hardy fly called a biting midge may play an important role in spreading vesicular stomatitis virus (VSV), which infects cattle, horses and swine, according to Agricultural Research Service (ARS) microbiologist Barbara Drolet.
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VSV causes significant economic losses to the livestock industry from sickened animals, quarantines and subsequent export/import restrictions.
Drolet, at the ARS Arthropod-Borne Animal Diseases Research Laboratory in Laramie, Wyo., and colleagues now have proof that VSV is capable of surviving and spreading throughout the blood-sucking midge, Culicoides sonorensis. To verify that Culicoides propagated VSV, the scientists had to find a way to show, without killing the midge, that virus ingested in a blood meal could survive the insect's midgut, then replicate and escape from the midgut to infect other organs.
Using an artificial feeding system, Drolet fed midges a viral meal and tracked the ensuing infections over time to show that VSV infects the midge's salivary glands and eggs and is shed in the midge's droppings. Drolet used a genetic technique to verify that the virus is able to replicate within the insect.


