Featured Research

from universities, journals, and other organizations

Building A Better DNA Molecule

Date:
May 27, 2008
Source:
Weizmann Institute of Science
Summary:
Scientists have demonstrated that a mathematical concept called recursion can be applied to constructing flawless synthetic DNA molecules. The ideal molecules are created in successive rounds in which faultless segments are lifted from longer, error-containing DNA strands and assembled anew.

Building faultless objects from faulty components may seem like alchemy. Yet scientists from the Weizmann Institute’s Computer Science and Applied Mathematics, and Biological Chemistry Departments have achieved just that, using a mathematical concept called recursion. 'We all use recursion, intuitively, to compose and comprehend sentences like ‘the dog that chases the cat that bit the mouse that ate the cheese that the man dropped is black,’' says Prof. Ehud Shapiro.

Recursion allows long DNA molecules to be composed hierarchically from smaller building blocks. But synthetic DNA building blocks have random errors within their sequence, as do the resulting molecules. Correcting these errors is necessary for the molecules to be useful. Even though the synthetic molecules are error prone, some of them are likely to have long stretches that do not contain any faults.

These stretches of faultless DNA can be identified, extracted, and reused in another round of recursive construction. Starting from longer and more accurate building blocks in this round increases the chances of producing a flawless long DNA molecule. The team, led by doctoral students Gregory Linshiz and Tuval Ben-Yehezkel under the supervision of Shapiro, found in their experiments that two rounds of recursive construction were enough to produce a flawless target DNA molecule. If need be, however, the error correction procedure could be repeated until the desired molecule is formed.

The team’s research, recently published in the journal Molecular Systems Biology, provides a novel way to construct faultless DNA molecules with greater speed, precision, and ease of combining synthetic and natural DNA fragments than existing methods. 'Synthetic DNA molecules are widely needed in bio-logical and biomedical research, and we hope that their efficient and accurate construction using this recursive process will help to speed up progress in these fields,' says Shapiro.

Prof. Ehud Shapiro’s research is supported by the Clore Center for Biological Physics; the Arie and Ida Crown Memorial Charitable Fund; the Cymerman - Jakubskind Prize; the Fusfeld Research Fund; the Phyllis and Joseph Gurwin Fund for Scientific Advancement; the Henry Gutwirth Fund for Research; Ms. Sally Leafman Appelbaum, Scottsdale, AZ; the Carolito Stiftung, Switzerland; the Louis Chor Memorial Trust Fund; and the estate of Fannie Sherr, New York, NY. Prof. Shapiro is the incumbent of the Harry Weinrebe Chair of Computer Science and Biology.


Story Source:

The above story is based on materials provided by Weizmann Institute of Science. Note: Materials may be edited for content and length.


Cite This Page:

Weizmann Institute of Science. "Building A Better DNA Molecule." ScienceDaily. ScienceDaily, 27 May 2008. <www.sciencedaily.com/releases/2008/05/080527094154.htm>.
Weizmann Institute of Science. (2008, May 27). Building A Better DNA Molecule. ScienceDaily. Retrieved August 30, 2014 from www.sciencedaily.com/releases/2008/05/080527094154.htm
Weizmann Institute of Science. "Building A Better DNA Molecule." ScienceDaily. www.sciencedaily.com/releases/2008/05/080527094154.htm (accessed August 30, 2014).

Share This




More Plants & Animals News

Saturday, August 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

We've Got Mites Living In Our Faces And So Do You

We've Got Mites Living In Our Faces And So Do You

Newsy (Aug. 30, 2014) A new study suggests 100 percent of adult humans (those over 18 years of age) have Demodex mites living in their faces. Video provided by Newsy
Powered by NewsLook.com
Experimental Ebola Drug ZMapp Cures Lab Monkeys Of Disease

Experimental Ebola Drug ZMapp Cures Lab Monkeys Of Disease

Newsy (Aug. 29, 2014) In a new study, a promising experimental treatment for Ebola managed to cure a group of infected macaque monkeys. Video provided by Newsy
Powered by NewsLook.com
Killer Amoeba Found in Louisiana Water System

Killer Amoeba Found in Louisiana Water System

AP (Aug. 28, 2014) State health officials say testing has confirmed the presence of a killer amoeba in a water system serving three St. John the Baptist Parish towns. (Aug. 28) Video provided by AP
Powered by NewsLook.com
Raw: Australian Sheep Gets Long Overdue Haircut

Raw: Australian Sheep Gets Long Overdue Haircut

AP (Aug. 28, 2014) Hoping to break the record for world's wooliest, Shaun the sheep came up 10 pounds shy with his fleece weighing over 50 pounds after being shorn for the first time in years. (Aug. 28) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins