Featured Research

from universities, journals, and other organizations

Wealth Of Genomic Hotspots Discovered In Embryonic Stem Cells

Date:
June 15, 2008
Source:
Agency for Science, Technology and Research (A*STAR), Singapore
Summary:
Singapore scientists unveil an atlas showing the location of "genomic hotspots" of essential protein "switches" (transcription factors) that help maintain the embryonic stem cell state. Using advanced high throughput sequencing technology, the scientists discovered over 3,000 hotspots. These findings could improve understanding of the unique properties of stem cells that enable them to maintain their intriguing ability to grow and differentiate to virtually any cell type.

Singapore scientists at the Genome Institute of Singapore (GIS) and the National University of Singapore (NUS) have unveil an atlas that showing the location of "genomic hotspots" of essential protein "switches" (transcription factors) that are critical for maintaining the embryonic stem (ES) cell state.

Using advanced high throughput sequencing technology, the scientists discovered over 3,000 hotspots. These findings could improve understanding of the unique properties of stem cells that enable them to maintain their intriguing ability to grow and differentiate to virtually any cell type.

"This is the first time such a large scale study has been conducted in Singapore and obtaining such groundbreaking results has caused much excitement," said Wei Chia Lin, Ph.D., Senior Group Leader at GIS. "This blueprint that we obtained is like a treasure map, pointing us to specific sites where we can further study how these switches interact within the cell. Hopefully, this will eventually allow us unlock the secrets of stem cells."

Ng Huck Hui, Ph.D., also a Senior Group Leader at GIS, added, "we think that these 'stemness' hotspots are the most critical points in the genetic blueprint of ES cells. By targeting these hotspots, we may be able to reconnect the wiring in non-stem cells and jump-start the stem cell program in them. This can potentially create an inexhaustible source of clinically useful cells for regenerative medicine or cell based therapies in the future." The team has already started work to investigate further into this area of research.

"Using cutting edge sequencing technology, scientists from the GIS and NUS have identified hotspots in embryonic stem cells," said Prof. Lee Eng Hin, Executive Director of A*STAR's Biomedical Research Council. "These are important hubs of the genome of embryonic stem cells. This piece of work illustrates how scientists from different disciplines and across institutions can come together to define fundamental features of these intriguing cells."

"In this new paper in Cell, the team at the GIS continues their remarkable progress in defining the precise DNA sequences to which an important group of 13 transcriptional factors bind in mouse embryonic stem cells," said Alan Colman, Ph.D., Executive Director of Singapore's Stem Cell Consortium. "This particular group of factors is responsible for maintaining the self renewal and pluripotency of the embryonic stem cells. The team shows that many of the factors which bind to the same gene regions ('hotspots') and their work provide a working model of the transcriptional networks at play within the cells, and how these intracellular networks are linked to events that can be influenced by external stimuli."

The researchers performed genome-wide mapping of the in vivo binding sites for 13 sequence-specific transcription factors in ES cells. These transcription factors play different roles in self-renewal, pluripotency, reprogramming and chromatin insulation. This study uncovers two major modes of binding that give rise to transcription factor co- localization hotspots. The Nanog/Oct4/Sox2 centric hotspots are commonly co-bound by Smad1 and STAT3 and they represent points of integration for the intrinsic and external signaling pathways. The combinatorial wiring of transcription factors is important in deciphering the code behind gene expression program in ES cells.

The work done by the GIS team is a follow up on a series of ongoing research ( "The Oct4 and Nanog transcription network that regulates pluripotency in mouse embroynic stem cells," Nature Genetics 38:431-440, 2006) into understanding and mapping the transcriptional networks of master genes in ES and somatic cells.


Story Source:

The above story is based on materials provided by Agency for Science, Technology and Research (A*STAR), Singapore. Note: Materials may be edited for content and length.


Journal Reference:

  1. Integration of external signaling pathways with the core transcriptional network in embryonic stem cells. Cell, June 13, 2008

Cite This Page:

Agency for Science, Technology and Research (A*STAR), Singapore. "Wealth Of Genomic Hotspots Discovered In Embryonic Stem Cells." ScienceDaily. ScienceDaily, 15 June 2008. <www.sciencedaily.com/releases/2008/06/080613115331.htm>.
Agency for Science, Technology and Research (A*STAR), Singapore. (2008, June 15). Wealth Of Genomic Hotspots Discovered In Embryonic Stem Cells. ScienceDaily. Retrieved October 22, 2014 from www.sciencedaily.com/releases/2008/06/080613115331.htm
Agency for Science, Technology and Research (A*STAR), Singapore. "Wealth Of Genomic Hotspots Discovered In Embryonic Stem Cells." ScienceDaily. www.sciencedaily.com/releases/2008/06/080613115331.htm (accessed October 22, 2014).

Share This



More Health & Medicine News

Wednesday, October 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

CDC Revamps Ebola Guidelines After Criticism

CDC Revamps Ebola Guidelines After Criticism

Newsy (Oct. 21, 2014) The Centers for Disease Control and Prevention have issued new protocols for healthcare workers interacting with Ebola patients. Video provided by Newsy
Powered by NewsLook.com
WHO: Ebola Vaccine Trials to Start a in January

WHO: Ebola Vaccine Trials to Start a in January

AP (Oct. 21, 2014) Tens of thousands of doses of experimental Ebola vaccines could be available for "real-world" testing in West Africa as soon as January as long as they are deemed safe in soon to start trials, the World Health Organization said Tuesday. (Oct. 21) Video provided by AP
Powered by NewsLook.com
First-Of-Its-Kind Treatment Gives Man Ability To Walk Again

First-Of-Its-Kind Treatment Gives Man Ability To Walk Again

Newsy (Oct. 21, 2014) A medical team has for the first time given a man the ability to walk again after transplanting cells from his brain onto his severed spinal cord. Video provided by Newsy
Powered by NewsLook.com
CDC Issues New Ebola Guidelines for Health Workers

CDC Issues New Ebola Guidelines for Health Workers

Reuters - US Online Video (Oct. 21, 2014) The U.S. Centers for Disease Control and Prevention has set up new guidelines for health workers taking care of patients infected with Ebola. Linda So reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins