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Tick-borne Encephalitis Virus Reveals Its Access Code

Date:
October 22, 2008
Source:
Rockefeller University Press
Summary:
Biologists have identified an amino acid switch that flaviviruses flip to gain access to cells. Flaviviruses such as tick-borne encephalitis virus (TBEV), yellow fever, and dengue are dangerous human pathogens.

Fritz et al. have identified an amino acid switch that flaviviruses flip to gain access to cells.

Flaviviruses such as tick-borne encephalitis virus (TBEV), yellow fever, and dengue are dangerous human pathogens. These membrane-encircled viruses enter cells by being gobbled up into endosomes and fusing their membrane with that of the endosome.

Fusion is triggered by the endosome's acidic environment. Low pH prompts the aptly named fusion protein, on the virus's outer membrane, to change shape and grab hold of the endosome membrane, bringing the two membranes together. In their search for possible pH sensors, researchers have focused on five highly conserved histidine residues in the flavivirus fusion protein. The chemical properties of histidines make them prime candidates—they switch from uncharged to having a double positive charge upon acidification of their environment, such as that in endosomes.

Fritz et al. replaced each of the five histidines of the TBEV fusion protein with alternative residues and observed the virus's fusion ability. Given the conservation of the five histidines, the team was surprised, that mutation of one of the histidines, His323, was sufficient to completely abolish fusion. Individual mutation of three of the others had no effect on fusion whatsoever, and mutation of the fourth led to an untestable ill-formed fusion protein.

The team went on to show that mutation of the crucial His323 interfered with the pH-induced shape change of the fusion protein.


Story Source:

The above story is based on materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Journal Reference:

  1. Fritz et al. Identification of specific histidines as pH sensors in flavivirus membrane fusion. The Journal of Cell Biology, 2008; 183 (2): 353 DOI: 10.1083/jcb.200806081

Cite This Page:

Rockefeller University Press. "Tick-borne Encephalitis Virus Reveals Its Access Code." ScienceDaily. ScienceDaily, 22 October 2008. <www.sciencedaily.com/releases/2008/10/081020093356.htm>.
Rockefeller University Press. (2008, October 22). Tick-borne Encephalitis Virus Reveals Its Access Code. ScienceDaily. Retrieved April 18, 2014 from www.sciencedaily.com/releases/2008/10/081020093356.htm
Rockefeller University Press. "Tick-borne Encephalitis Virus Reveals Its Access Code." ScienceDaily. www.sciencedaily.com/releases/2008/10/081020093356.htm (accessed April 18, 2014).

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