Featured Research

from universities, journals, and other organizations

2D protein maps of mucosal biopsies in patients with ileal pouch-anal anastomosis

Date:
January 14, 2010
Source:
World Journal of Gastroenterology
Summary:
A group of researchers utilized a comparative proteomic approach to profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment.

A group of researchers headed by Professors Patrizia Brigidi and Massimo Campieri utilized a comparative proteomic approach to profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment.

Total proctocolectomy with ileal J-pouch-anal anastomosis (IPAA) is the surgical treatment of choice for patients with refractory ulcerative colitis (UC). Although the surgery generally cures UC allowing a significant improvement of health-related quality of life, complications can occur after IPAA. The most common complication of this surgery is pouchitis, a non-specific inflammatory condition at the ileal pouch reservoir occurring in up to 50% of patients, with 10%-15% of these patients becoming chronic sufferers. Although the etiology and pathogenesis of pouchitis are still not entirely clear, the bulk of the evidence points towards an abnormal mucosal immune response to altered microbiota patterns.

The management of pouchitis is largely empirical and only a few small placebo-controlled clinical trials have been conducted. Although antibiotics represent the mainstay of treatment, probiotics have recently gained more attention as an effective therapeutic option for pouchitis management. However, little is known about the molecular mechanisms underlying the protective effects of probiotics against pouchitis onset.

A research article to be published on January 7, 2010 in the World Journal of Gastroenterology addresses this question. A study group led by Dr. Patrizia Brigidi from Italy, profiled protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment, using a comparative proteomic approach. For the first time, 2D protein maps of mucosal biopsy samples from patients who underwent IPAA are provided. In the first set of 2D gels, 26 different proteins with at least 2-fold changes in their expression levels between the pouchitis condition and antibiotic-induced remission were identified. In the second set of analysis, the comparison between mucosal biopsies proteomes in the normal and probiotic-treated pouch resulted in 17 significantly differently expressed proteins. Of these, 8 exhibited the same pattern of deregulation as in the pouchitis/pouch remission group.

After classifying the identified proteins on the basis of the biological functions, the authors give an overall theoretical analysis. Their findings suggest the involvement of enterocyte mitochondrial dysfunction and perturbed cytoskeletal structure into pouchitis pathophysiology. The modulation of metabolic pathways and the effects on cell integrity and morphology in response to probiotic administration, provide new insights into the molecular interaction between probiotics and the altered host intestinal ecosystem in IPAA.

The proteomic technologies could play a major role in unraveling the mystery of immunopathogenic mechanisms of pouchitis and in discovering novel biomarkers for disease activity, diagnosis and prognosis.


Story Source:

The above story is based on materials provided by World Journal of Gastroenterology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Turroni S, Vitali B, Candela M, Gionchetti P, Rizzello F, Campieri M, Brigidi P. Antibiotics and probiotics in chronic pouchitis: A comparative proteomic approach. World J Gastroenterol, 2010; 16(1): 30-41 [link]

Cite This Page:

World Journal of Gastroenterology. "2D protein maps of mucosal biopsies in patients with ileal pouch-anal anastomosis." ScienceDaily. ScienceDaily, 14 January 2010. <www.sciencedaily.com/releases/2010/01/100114091208.htm>.
World Journal of Gastroenterology. (2010, January 14). 2D protein maps of mucosal biopsies in patients with ileal pouch-anal anastomosis. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2010/01/100114091208.htm
World Journal of Gastroenterology. "2D protein maps of mucosal biopsies in patients with ileal pouch-anal anastomosis." ScienceDaily. www.sciencedaily.com/releases/2010/01/100114091208.htm (accessed July 31, 2014).

Share This




More Health & Medicine News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Health Insurers' Profits Slide

Health Insurers' Profits Slide

Reuters - Business Video Online (July 30, 2014) Obamacare-related costs were said to be behind the profit plunge at Wellpoint and Humana, but Wellpoint sees the new exchanges boosting its earnings for the full year. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
At Least 20 Chikungunya Cases in New Jersey

At Least 20 Chikungunya Cases in New Jersey

AP (July 30, 2014) At least 20 New Jersey residents have tested positive for chikungunya, a mosquito-borne virus that has spread through the Caribbean. (July 30) Video provided by AP
Powered by NewsLook.com
Xtreme Eating: Your Daily Caloric Intake All On One Plate

Xtreme Eating: Your Daily Caloric Intake All On One Plate

Newsy (July 30, 2014) The Center for Science in the Public Interest released its 2014 list of single meals with whopping calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins