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Molecular basis for Pseudomonas aeruginosa persistent infections in CF patients

Date:
April 21, 2010
Source:
Public Library of Science
Summary:
New research reveals Small Colony Variants (SCVs) of P. aeruginosa to be a hallmark of chronic infection in cystic fibrosis (CF) patients. Results suggest that SCV-mediated persistence might be a good target for antimicrobial chemotherapy.
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New research reveals Small Colony Variants (SCVs) of P. aeruginosa to be a hallmark of chronic infection in cystic fibrosis (CF) patients. Results, published March 12th in the open-access journal PLoS Pathogens, suggest that SCV-mediated persistence might be a good target for antimicrobial chemotherapy.

Cystic fibrosis is a widespread genetic disease that leads to progressive disability and early death. The principal cause of mortality and morbidity in CF patients is a progressive deterioration of the respiratory system caused by a chronic infection of the patients' lungs, mainly by the opportunistic bacterial pathogen Pseudomonas aeruginosa. CF lung infections can be treated with antibiotics, however full clearance is not possible due to the protective environment of the CF lung and the adaptation of infective species to a persistent lifestyle. This presents serious challenges for the long-term chemotherapy of CF patients.

Adaptive P. aeruginosa morphotypes include SCVs, slow-growing and strongly adherent variants that frequently arise in chronic lung infections. Because the appearance of SCVs correlates with poor lung function and antibiotic resistance, they have long been suspected of mediating the P. aeruginosa persistence phenotype in CF infections.

In this study, the researchers characterized a signaling system in P. aeruginosa called YfiBNR, mutations in which lead to the generation of SCV variants. Activation of YfiBNR resulted in increased levels of the signaling molecule c-di-GMP, which in turn triggered massive production of exopolysaccharides and drastically reduced growth rates, two hallmarks of SCV behavior. YfiN-mediated SCVs were shown to be highly resistant to macrophage phagocytosis, suggesting a role for the SCV phenotype in immune system evasion. Consistent with this, activation of YfiN significantly increased the persistence of P. aeruginosa in long-term infections in a mouse model, establishing a firm causal link between SCV and persistence in chronic P. aeruginosa infections.

The authors conclude that 'c-di-GMP has long been a key suspect in chronic behavior of bacterial pathogens. The finding that the c-di-GMP-mediated SCV phenotype confers a persistent advantage in mice provides the first direct evidence in favor of such a model. This study thus opens up new avenues to specifically counteract persistent infections.'


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The above story is based on materials provided by Public Library of Science. Note: Materials may be edited for content and length.


Journal Reference:

  1. Malone JG, Jaeger T, Spangler C, Ritz D, Spang A, et al. YfiBNR Mediates Cyclic di-GMP Dependent Small Colony Variant Formation and Persistence in Pseudomonas aeruginosa. PLoS Pathogens, 2010; 6 (3): e1000804 DOI: 10.1371/journal.ppat.1000804

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Public Library of Science. "Molecular basis for Pseudomonas aeruginosa persistent infections in CF patients." ScienceDaily. ScienceDaily, 21 April 2010. <www.sciencedaily.com/releases/2010/03/100311202721.htm>.
Public Library of Science. (2010, April 21). Molecular basis for Pseudomonas aeruginosa persistent infections in CF patients. ScienceDaily. Retrieved May 30, 2015 from www.sciencedaily.com/releases/2010/03/100311202721.htm
Public Library of Science. "Molecular basis for Pseudomonas aeruginosa persistent infections in CF patients." ScienceDaily. www.sciencedaily.com/releases/2010/03/100311202721.htm (accessed May 30, 2015).

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