Featured Research

from universities, journals, and other organizations

Certain laboratory technique allows rapid detection of eye pathogens

Date:
May 14, 2010
Source:
JAMA and Archives Journals
Summary:
A laboratory technique using real-time polymerase chain reaction that copies DNA segments may allow clinicians to accurately identify pathogens infecting the cornea more quickly than standard methods, according to a new study.

A laboratory technique using real-time polymerase chain reaction (PCR) that copies DNA segments may allow clinicians to accurately identify pathogens infecting the cornea more quickly than standard methods, according to a report in the May issue of Archives of Ophthalmology, one of the JAMA/Archives journals.

"Corneal ulcer, including bacterial keratitis, fungal keratitis and Acanthamoeba keratitis, can cause corneal opacity, deteriorated visual acuity or even lead to some lifelong complications," the authors write as background information in the article. "Bacterial culture and smear examination using corneal scrapings is the conventional method to detect causative pathogens of corneal ulcer. However, bacterial culture is time-consuming and results of smear examination depend on the laboratory technician's skill. Therefore, a fast and accurate diagnostic method is highly desirable."

In recent years, PCR has become more widely used clinically for the detection of bacteria and viruses; it amplifies a small segment of DNA for assessment. Motoki Itahashi, M.D., Ph.D., and colleagues at Kinki University School of Medicine, Osaka-Sayama, Japan, compared real-time PCR using corneal scrapings to bacterial culture for the detection of six common bacteria and fungi in 40 eyes of 40 patients diagnosed with corneal ulcer.

The real-time PCR assay delivered results within two hours, whereas culture results were examined after 48 hours. Of the 40 eyes, 20 had the same pathogens detected by both methods and six showed negative results by both methods. Results differed in 14 eyes: 11 eyes had positive results for one of the six pathogens only on PCR, two had positive results on culture only and one eye had positive results for two different pathogens.

"Although PCR has a high risk of false positivity, we actually treated the patients with positive PCR results only according to their real-time PCR results and the treatment outcomes were all satisfactory," the authors write. "This may demonstrate a better detection sensitivity in the PCR assay."

"In conclusion, though the numbers included in this study were limited, particularly with fungal ulcers, we have demonstrated that real-time PCR can accurately and simultaneously detect bacterial and fungal pathogens in a speedy fashion," the authors write. "With real-time PCR, it may be possible to develop a diagnostic kit for pathogen-specific detection in the busy ophthalmic clinical practice."


Story Source:

The above story is based on materials provided by JAMA and Archives Journals. Note: Materials may be edited for content and length.


Journal Reference:

  1. Motoki Itahashi; Shiro Higaki; Masahiko Fukuda; Yoshikazu Shimomura. Detection and Quantification of Pathogenic Bacteria and Fungi Using Real-Time Polymerase Chain Reaction by Cycling Probe in Patients With Corneal Ulcer. Arch Ophthalmol, 2010; 128 (5): 535-540 [link]

Cite This Page:

JAMA and Archives Journals. "Certain laboratory technique allows rapid detection of eye pathogens." ScienceDaily. ScienceDaily, 14 May 2010. <www.sciencedaily.com/releases/2010/05/100510161300.htm>.
JAMA and Archives Journals. (2010, May 14). Certain laboratory technique allows rapid detection of eye pathogens. ScienceDaily. Retrieved August 21, 2014 from www.sciencedaily.com/releases/2010/05/100510161300.htm
JAMA and Archives Journals. "Certain laboratory technique allows rapid detection of eye pathogens." ScienceDaily. www.sciencedaily.com/releases/2010/05/100510161300.htm (accessed August 21, 2014).

Share This




More Health & Medicine News

Thursday, August 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Newsy (Aug. 21, 2014) An experimental drug used to treat Marburg virus in rhesus monkeys could give new insight into a similar treatment for Ebola. Video provided by Newsy
Powered by NewsLook.com
Cadavers, a Teen, and a Medical School Dream

Cadavers, a Teen, and a Medical School Dream

AP (Aug. 21, 2014) Contains graphic content. He's only 17. But Johntrell Bowles has wanted to be a doctor from a young age, despite the odds against him. He was recently the youngest participant in a cadaver program at the Indiana University NW medical school. (Aug. 21) Video provided by AP
Powered by NewsLook.com
American Ebola Patients Released: What Cured Them?

American Ebola Patients Released: What Cured Them?

Newsy (Aug. 21, 2014) It's unclear whether the American Ebola patients' recoveries can be attributed to an experimental drug or early detection and good medical care. Video provided by Newsy
Powered by NewsLook.com
Lost Brain Cells To Blame For Sleep Problems Among Seniors

Lost Brain Cells To Blame For Sleep Problems Among Seniors

Newsy (Aug. 21, 2014) According to a new study, elderly people might have trouble sleeping because of the loss of a certain group of neurons in the brain. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins