Featured Research

from universities, journals, and other organizations

Platform developed to monitor hematopoietic stem cells

Date:
May 23, 2011
Source:
University of British Columbia
Summary:
Researchers have developed an automated microfluidic cell culture platform to monitor the growth, survival and responses of hundreds of hematopoietic stem cells (HSCs) at the single cell level.

A Canadian research team has developed an automated microfluidic cell culture platform to monitor the growth, survival and responses of hundreds of hematopoietic stem cells (HSCs) at the single cell level.

This new tool allows scientists to study multiple temporally varying culture conditions simultaneously and to gain new insights on the growth factor requirements for HSC survival.

"The ability to perform massively parallel cultures of single non-adherent mammalian cells will provide new avenues to explore complex biological questions," says Vιronique Lecault, lead author of the study and a PhD candidate in the UBC Dept. of Chemical and Biological Engineering.

"Our results will find use in broader applications such as drug development, clone selection and culture optimization," says Lecault.

The findings appear in the May 22 issue of the online journal Nature Methods. The study is a collaborative project between the laboratories of Asst. Prof. Carl Hansen, UBC Physics and Astronomy, Centre for High-Throughput Biology, Prof. James Piret, UBC Chemical and Biological Engineering, Michael Smith Laboratories, Prof. Connie Eaves, Terry Fox Laboratory, BC Cancer Agency, and Dr. Keith Humphries, Terry Fox Laboratory, BC Cancer Agency.

Lecault explains that HSCs are found mainly in adult bone marrow and have the astounding ability to sustain the continuous production of specialized blood cells.

These cells have major clinical implications, in particular for the treatment of cancer and blood-borne diseases, but the mechanisms regulating their division into stem cells (self-renewal) or more mature cells (differentiation) are not very well understood.

The heterogeneous nature of hematopoietic populations further complicates the study of these rare HSCs by hiding individual responses into average measurements. Single cell studies are therefore critical to elucidate these mechanisms but current techniques are labour intensive, require expensive reagents and provide limited flexibility to characterize cells or exchange culture conditions.

The team designed and fabricated microfluidic devices -- about the size of a matchbox -- containing 1,600 to 6,400 miniature culture chambers that can sustain robust cell growth, along with an automated time-lapse imaging system to track clones over multiple days as they expand from single cells.

"There are many challenges associated with the culture of suspension cells in nanolitre volumes including dehydration, nutrient limitations, and rapid variations if culture conditions are not well controlled," says Lecault.

The team was able to solve these problems by integrating an osmotic bath to block evaporation combined with a unique geometry that allows for automated medium exchange, immunostaining on live clones and cell recovery.


Story Source:

The above story is based on materials provided by University of British Columbia. Note: Materials may be edited for content and length.


Journal Reference:

  1. Vιronique Lecault, Michael VanInsberghe, Sanja Sekulovic, David J H F Knapp, Stefan Wohrer, William Bowden, Francis Viel, Thomas McLaughlin, Asefeh Jarandehei, Michelle M Miller, Didier Falconnet, Adam K White, David G Kent, Michael R Copley, Fariborz Taghipour, Connie J Eaves, R Keith Humphries, James M Piret, Carl L Hansen. High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays. Nature Methods, 2011; DOI: 10.1038/nmeth.1614

Cite This Page:

University of British Columbia. "Platform developed to monitor hematopoietic stem cells." ScienceDaily. ScienceDaily, 23 May 2011. <www.sciencedaily.com/releases/2011/05/110522141558.htm>.
University of British Columbia. (2011, May 23). Platform developed to monitor hematopoietic stem cells. ScienceDaily. Retrieved April 21, 2014 from www.sciencedaily.com/releases/2011/05/110522141558.htm
University of British Columbia. "Platform developed to monitor hematopoietic stem cells." ScienceDaily. www.sciencedaily.com/releases/2011/05/110522141558.htm (accessed April 21, 2014).

Share This



More Plants & Animals News

Monday, April 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Breakfast Foods Are Getting Pricier

Breakfast Foods Are Getting Pricier

AP (Apr. 21, 2014) — Breakfast is now being served with a side of sticker shock. The cost of morning staples like bacon, coffee and orange juice is on the rise because of global supply problems. (April 21) Video provided by AP
Powered by NewsLook.com
Mich. Boy Unearths 10,000-Year-Old Mastodon Tooth

Mich. Boy Unearths 10,000-Year-Old Mastodon Tooth

Newsy (Apr. 20, 2014) — A 9-year-old Michigan boy was exploring a creek when he came across a 10,000-year-old tooth from a prehistoric mastodon. Video provided by Newsy
Powered by NewsLook.com
Vermont Goat Meat Gives Refugees Taste of Home

Vermont Goat Meat Gives Refugees Taste of Home

AP (Apr. 18, 2014) — Dairy farmers and ethnic groups in Vermont are both benefiting from a unique collaborative effort that's feeding a growing need for fresh and affordable goat meat. (April 18) Video provided by AP
Powered by NewsLook.com
Man Claims He Found Loch Ness Monster With... Apple Maps?

Man Claims He Found Loch Ness Monster With... Apple Maps?

Newsy (Apr. 18, 2014) — Andy Dixon showed the Daily Mail a screenshot of what he believes to be the mythical beast swimming just below the lake's surface. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins