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Physicist detects movement of macromolecules engineered into our food

Date:
September 12, 2011
Source:
University of Montreal
Summary:
Toxin proteins are genetically engineered into our food because they kill insects by perforating body cell walls, and now researchers studying membrane proteins have detected the molecular mechanism involved.

Toxin proteins are genetically engineered into our food because they kill insects by perforating body cell walls, and Professor Rikard Blunck of the University of Montreal's Group for the study of membrane proteins (GΙPROM) has detected the molecular mechanism involved.

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In recognition of his breakthrough, he recently received the Traditional Paul F. Cranefield Award of the Society of General Physiologists. "This study is about gaining a better understanding of the basic functioning of the toxin proteins in order to judge the risks of using them as pesticides for our nutrition," Dr. Blunck explained.

The Cry1Aa toxin of B. thuringiensis that was investigated is a member of the class of proteins which are called "pore-forming toxins" because they perforate the walls, or membranes, of cells. Cry toxins kill insect larvae if ingested by them and are, therefore, genetically engineered into a number of transgenic crops, including those for human consumption, to make them resistant against these insects.

The pores in the membranes cause minerals necessary for the cell to live to break out and collapse the energy household of the cell. While these toxins could be studied outside of cell membranes through existing techniques that provide images of the 3D structure, the toxins rapidly change their architecture once in contact with the membrane, where the traditional approaches cannot be applied.

Dr. Blunck and his co-workers found a way of using fluorescent light to analyze the architecture and mechanism of the proteins in an artificial cell wall environment. Planar lipid bilayer (PLB) are artificial 0.1 mm-wide systems that mimic the cell membrane. The researchers developed a chip to investigate proteins introduced into these artificial cell walls with fluorescent light waves. Molecular fluorescent probes are coupled to the toxin proteins. If the proteins now enter the artificial membranes and change their structure, their architecture and movement and even their distribution can be followed -- thanks to the developed technique -- by the fluorescent light they are emitting.

"By watching the toxin in both its active and inactive state, and by measuring the dynamic changes of the light emitted by the molecular probes, we were able to determine which parts of it were interacting with the membrane to cause the pores." Dr. Blunck explained. "We expect the technique to be applied to a wide range of disease-causing toxins in future."


Story Source:

The above story is based on materials provided by University of Montreal. Note: Materials may be edited for content and length.


Journal Reference:

  1. N. Groulx, M. Juteau, R. Blunck. Rapid topology probing using fluorescence spectroscopy in planar lipid bilayer: the pore-forming mechanism of the toxin Cry1Aa of Bacillus thuringiensis. The Journal of General Physiology, 2010; 136 (5): 497 DOI: 10.1085/jgp.200910347

Cite This Page:

University of Montreal. "Physicist detects movement of macromolecules engineered into our food." ScienceDaily. ScienceDaily, 12 September 2011. <www.sciencedaily.com/releases/2011/09/110912102126.htm>.
University of Montreal. (2011, September 12). Physicist detects movement of macromolecules engineered into our food. ScienceDaily. Retrieved October 31, 2014 from www.sciencedaily.com/releases/2011/09/110912102126.htm
University of Montreal. "Physicist detects movement of macromolecules engineered into our food." ScienceDaily. www.sciencedaily.com/releases/2011/09/110912102126.htm (accessed October 31, 2014).

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