Featured Research

from universities, journals, and other organizations

Live cells 'printed' using standard inkjet printer

Date:
March 16, 2012
Source:
The Journal of Visualized Experiments
Summary:
Researchers have found a way to create temporary holes in the membranes of live cells using a standard inkjet printer. Creating temporary pores allow researchers to put molecules inside of cells that wouldn't otherwise fit, and study how the cells react.

Researchers from Clemson University have found a way to create temporary holes in the membranes of live cells using a standard inkjet printer.
Credit: Image courtesy of The Journal of Visualized Experiments

Researchers from Clemson University have found a way to create temporary holes in the membranes of live cells using a standard inkjet printer.

The method will be published in JoVE, the Journal of Visualized Experiments, on March 16.

"We first had the idea for this method when we wanted to be able to visualize changes in the cytoskeleton arrangement due to applied forces on cells," said paper-author Dr. Delphine Dean.

She said other researchers have been using this method to print cells onto slides, but that they have only recently discovered that printing the cells causes the disruption in their membranes for a few hours. Creating temporary pores allow researchers to put molecules inside of cells that wouldn't otherwise fit, and study how the cells react.

"The authors have used an extremely innovative approach for bioprinting cells. Moreover, this approach can be used for applications other than cell printing," said JoVE Science Editor, Dr. Nandita Singh. "Matrix proteins can be printed onto substrates with this technique for cell patterning. This JoVE publication will make this approach simple and approachable and enable other labs to replicate the procedure."

The printer is modified by removing the paperfeed mechanism and adding a "stage" from which to feed the slides. The ink is replaced with a cell solution, and the cells are printed directly on to the slides.

Using this method, the researchers are able to process thousands of cells in a matter of minutes. Dr. Dean's team used the holes to introduce fluorescent molecules that illuminate the skeleton of the cell.

"We are actually interested in the cell mechanics of compressed cells. This method allows us to push on the cells and watch the response easily," said Dr. Dean. "We are interested in cardiovascular cells, and how they respond to mechanical force."


Story Source:

The above story is based on materials provided by The Journal of Visualized Experiments. Note: Materials may be edited for content and length.


Journal Reference:

  1. Meaghan A. O'Reilly, Adam C. Waspe, Rajiv Chopra, Kullervo Hynynen. MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model. Journal of Visualized Experiments, 2012; (61) DOI: 10.3791/3555

Cite This Page:

The Journal of Visualized Experiments. "Live cells 'printed' using standard inkjet printer." ScienceDaily. ScienceDaily, 16 March 2012. <www.sciencedaily.com/releases/2012/03/120316112648.htm>.
The Journal of Visualized Experiments. (2012, March 16). Live cells 'printed' using standard inkjet printer. ScienceDaily. Retrieved August 22, 2014 from www.sciencedaily.com/releases/2012/03/120316112648.htm
The Journal of Visualized Experiments. "Live cells 'printed' using standard inkjet printer." ScienceDaily. www.sciencedaily.com/releases/2012/03/120316112648.htm (accessed August 22, 2014).

Share This




More Plants & Animals News

Friday, August 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Newsy (Aug. 21, 2014) — An experimental drug used to treat Marburg virus in rhesus monkeys could give new insight into a similar treatment for Ebola. Video provided by Newsy
Powered by NewsLook.com
Terrifying City-Dwelling Spiders Are Bigger And More Fertile

Terrifying City-Dwelling Spiders Are Bigger And More Fertile

Newsy (Aug. 21, 2014) — According to a new study, spiders that live in cities are bigger, fatter and multiply faster. Video provided by Newsy
Powered by NewsLook.com
Lost Brain Cells To Blame For Sleep Problems Among Seniors

Lost Brain Cells To Blame For Sleep Problems Among Seniors

Newsy (Aug. 21, 2014) — According to a new study, elderly people might have trouble sleeping because of the loss of a certain group of neurons in the brain. Video provided by Newsy
Powered by NewsLook.com
Ramen Health Risks: The Dark Side of the Noodle

Ramen Health Risks: The Dark Side of the Noodle

AP (Aug. 21, 2014) — South Koreans eat more instant ramen noodles per capita than anywhere else in the world. But American researchers say eating too much may increase the risk of diabetes, heart disease and stroke. (Aug. 21) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins