Featured Research

from universities, journals, and other organizations

Cholera discovery could revolutionize antibiotic delivery

Date:
October 19, 2012
Source:
Simon Fraser University
Summary:
Scientists have made a discovery that could help revolutionize antibiotic treatment of deadly bacteria. They have explained how Vibrio cholerae became a deadly pathogen thousands of years ago. Two genes within V. cholerae's genome make it toxic and deadly. The bacterium acquired these genes when a bacterial virus or bacteriophage called CTX-phi infected it.

Three Simon Fraser University scientists are among six researchers who've made a discovery that could help revolutionize antibiotic treatment of deadly bacteria.

Lisa Craig, Christopher Ford and Subramaniapillai Kolappan, SFU researchers in molecular biology and biochemistry, have explained how Vibrio cholerae became a deadly pathogen thousands of years ago.

V. cholerae causes the diarrheal disease cholera, which is endemic in many developing countries and can emerge in regions devastated by war and natural disasters. An outbreak following the 2010 earthquake in Haiti has killed at least 7,500 people.

Two genes within V. cholerae's genome make it toxic and deadly. The bacterium acquired these genes when a bacterial virus or bacteriophage called CTX-phi infected it.

The SFU researchers and their colleagues at the University of Oslo and Harvard Medical School propose that a Trojan horse-like mechanism within V. cholerae enabled CTX-phi to invade it.

The CTX-phi latches onto a long, hair-like pilus filament floating on the surface of V. cholerae. The filament then retracts, pulling the toxin-gene-carrying CTX-phi inside the bacterium where it binds to TolA, a protein in the bacterial wall.

The process transforms V. cholerae into a deadly human pathogen.

The Journal of Biological Chemistry has just published a paper written by the researchers describing the atomic structures of the CTX-phi protein pIII alone and bound to V. cholera TolA.

The authors recommend that pilus filaments be explored further as a transport mechanism to deliver antibiotics into a bacterium.

"We'd be exploiting the pilus retraction mechanism to introduce antibiotics directly into a cell, bypassing its outer membrane barrier," explains Craig. The SFU associate professor is an expert on the role that pili play in bacterial infections.

"We do have antibiotics for V. cholerae, but these antibiotics also kill beneficial bacteria in the gut. The idea of using pili as a Trojan horse for antibiotic delivery is new and allows us to specifically and effectively target a given bacterial pathogen."

Craig says her team's discovery of V. cholerae's retractable pili is made all the more exciting by the simplicity of its workings. "We know that other deadly bacteria have retractable pili but it'll be much easier to isolate how the mechanism can be used to uptake antibiotics in Vibrio cholerae."

Craig says using pili as an antibiotic delivery mechanism to treat Pseudomonas aeruginosa, a deadly bacterial respiratory infection that hits mainly people with Cystic Fibrosis, could save many lives.

Christopher Ford is a research associate in Craig's lab. Subramaniapillai Kolappan, one of Craig's master's students, recently graduated from SFU.


Story Source:

The above story is based on materials provided by Simon Fraser University. Note: Materials may be edited for content and length.


Journal Reference:

  1. C. G. Ford, S. Kolappan, H. T. H. Phan, M. K. Waldor, H. C. Winther-Larsen, L. Craig. Crystal Structures of a CTX pIII Domain Unbound and in Complex with a Vibrio cholerae TolA Domain Reveal Novel Interaction Interfaces. Journal of Biological Chemistry, 2012; 287 (43): 36258 DOI: 10.1074/jbc.M112.403386

Cite This Page:

Simon Fraser University. "Cholera discovery could revolutionize antibiotic delivery." ScienceDaily. ScienceDaily, 19 October 2012. <www.sciencedaily.com/releases/2012/10/121019153239.htm>.
Simon Fraser University. (2012, October 19). Cholera discovery could revolutionize antibiotic delivery. ScienceDaily. Retrieved April 20, 2014 from www.sciencedaily.com/releases/2012/10/121019153239.htm
Simon Fraser University. "Cholera discovery could revolutionize antibiotic delivery." ScienceDaily. www.sciencedaily.com/releases/2012/10/121019153239.htm (accessed April 20, 2014).

Share This



More Plants & Animals News

Sunday, April 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Vermont Goat Meat Gives Refugees Taste of Home

Vermont Goat Meat Gives Refugees Taste of Home

AP (Apr. 18, 2014) Dairy farmers and ethnic groups in Vermont are both benefiting from a unique collaborative effort that's feeding a growing need for fresh and affordable goat meat. (April 18) Video provided by AP
Powered by NewsLook.com
Man Claims He Found Loch Ness Monster With... Apple Maps?

Man Claims He Found Loch Ness Monster With... Apple Maps?

Newsy (Apr. 18, 2014) Andy Dixon showed the Daily Mail a screenshot of what he believes to be the mythical beast swimming just below the lake's surface. Video provided by Newsy
Powered by NewsLook.com
First Ever 'Female Penis' Discovered In Animal Kingdom

First Ever 'Female Penis' Discovered In Animal Kingdom

Newsy (Apr. 18, 2014) Not only are these newly discovered bugs' sex organs reversed, but they also mate for up to 70 hours. Video provided by Newsy
Powered by NewsLook.com
Little Progress Made In Fighting Food Poisoning, CDC Says

Little Progress Made In Fighting Food Poisoning, CDC Says

Newsy (Apr. 18, 2014) A new report shows rates of two foodborne infections increased in the U.S. in recent years, while salmonella actually dropped 9 percent. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins