Featured Research

from universities, journals, and other organizations

Family trees for yeast cells

Date:
May 13, 2013
Source:
Université du Luxembourg
Summary:
Researchers have developed a revolutionary method to analyze the genomes of yeast families.

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families.

Related Articles


The team of Dr. Aimée Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads.

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads." To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that underlie complex traits requires extremely large numbers of progeny and high volumes of DNA sequencing," says Patrick May. "BEST combines the power of a gold standard genetic technique with ultra-high-throughput genome sequencing in a way that will enable the study of problems that were previously not possible."


Story Source:

The above story is based on materials provided by Université du Luxembourg. Note: Materials may be edited for content and length.


Journal Reference:

  1. Catherine L Ludlow, Adrian C Scott, Gareth A Cromie, Eric W Jeffery, Amy Sirr, Patrick May, Jake Lin, Teresa L Gilbert, Michelle Hays, Aimée M Dudley. High-throughput tetrad analysis. Nature Methods, 2013; DOI: 10.1038/nmeth.2479

Cite This Page:

Université du Luxembourg. "Family trees for yeast cells." ScienceDaily. ScienceDaily, 13 May 2013. <www.sciencedaily.com/releases/2013/05/130513083051.htm>.
Université du Luxembourg. (2013, May 13). Family trees for yeast cells. ScienceDaily. Retrieved April 25, 2015 from www.sciencedaily.com/releases/2013/05/130513083051.htm
Université du Luxembourg. "Family trees for yeast cells." ScienceDaily. www.sciencedaily.com/releases/2013/05/130513083051.htm (accessed April 25, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Saturday, April 25, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Dispute Flares Over Controversial Thai Temple Tigers

Dispute Flares Over Controversial Thai Temple Tigers

AFP (Apr. 24, 2015) — Thai wildlife officials begin a headcount of nearly 150 tigers kept by monks at a temple which has become the centre of a dispute over the welfare of the animals. Video provided by AFP
Powered by NewsLook.com
College Kegger: University Gets in on Craft Brew

College Kegger: University Gets in on Craft Brew

AP (Apr. 24, 2015) — Theres never been a shortage of beer on college campuses. But students at Cal Poly-Pomona are learning how to brew, serving their product to classmates, and hoping to land jobs in craft breweries when they graduate. (April 24) Video provided by AP
Powered by NewsLook.com
Cambodian Butterflies Help Villagers Make a Living

Cambodian Butterflies Help Villagers Make a Living

AFP (Apr. 24, 2015) — Cambodia&apos;s Banteay Srey Butterfly Centre is the largest of its kind in Southeast Asia. As well as educating tourists about the creatures, it also offers a source of income to nearby villagers, who are paid to breed local species. Duration: 02:04 Video provided by AFP
Powered by NewsLook.com
3D Food Printing: The Meal of the Future?

3D Food Printing: The Meal of the Future?

AP (Apr. 23, 2015) — Developers of 3D food printing hope the culinary technology will revolutionize the way we cook and eat. (April 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins