Featured Research

from universities, journals, and other organizations

First-ever view of gene activity, transcript patterns in single human cells

Date:
October 6, 2013
Source:
University of Zurich
Summary:
Biologists have developed a method to visualize the activity of genes in single cells. The method is so efficient that, for the first time, a thousand genes can be studied in parallel in ten thousand single human cells. Applications lie in fields of basic research and medical diagnostics. The new method shows that the activity of genes, and the spatial organization of the resulting transcript molecules, strongly vary between single cells.

Biologists of the University of Zurich have developed a method to visualize the activity of genes in single cells. The method is so efficient that, for the first time, a thousand genes can be studied in parallel in ten thousand single human cells. Applications lie in fields of basic research and medical diagnostics. The new method shows that the activity of genes, and the spatial organization of the resulting transcript molecules, strongly vary between single cells.

Whenever cells activate a gene, they produce gene specific transcript molecules, which make the function of the gene available to the cell. The measurement of gene activity is a routine activity in medical diagnostics, especially in cancer medicine. Today’s technologies determine the activity of genes by measuring the amount of transcript molecules. However, these technologies can neither measure the amount of transcript molecules of one thousand genes in ten thousand single cells, nor the spatial organization of transcript molecules within a single cell. The fully automated procedure, developed by biologists of the University of Zurich under the supervision of Prof. Lucas Pelkmans, allows, for the first time, a parallel measurement of the amount and spatial organization of single transcript molecules in ten thousands single cells. The results, which were recently published in the scientific journal Nature Methods, provide completely novel insights into the variability of gene activity of single cells.

Robots, a fluorescence microscope and a supercomputer

The method developed by Pelkmans’ PhD students Nico Battich and Thomas Stoeger is based upon the combination of robots, an automated fluorescence microscope and a supercomputer. “When genes become active, specific transcript molecules are produced. We can stain them with the help of a robot”, explains Stoeger. Subsequently, fluorescence microscope images of brightly glowing transcript molecules are generated. Those images were analyzed with the supercomputer Brutus, of the ETH Zurich. With this method, one thousand human genes can be studied in ten thousand single cells. According to Pelkmans, the advantages of this method are the high number of single cells and the possibility to study, for the first time, the spatial organization of the transcript molecules of many genes.

New insights into the spatial organization of transcript molecules

The analysis of the new data shows that individual cells distinguish themselves in the activity of their genes. While the scientists had been suspecting a high variability in the amount of transcript molecules, they were surprised to discover a strong variability in the spatial organization of transcript molecules within single cells and between multiple single cells. The transcript molecules adapted distinctive patterns.

“We realized that genes with a similar function also have a similar variability in the transcript patterns,” explains Battich. “This similarity exceeds the variability in the amount of transcript molecules, and allows us to predict the function of individual genes.” The scientists suspect that transcript patterns are a countermeasure against the variability in the amount of transcript molecules. Thus, such patterns would be responsible for the robustness of processes within a cell.

The importance of these new insights was summarized by Pelkmans: “Our method will be of importance to basic research and the understanding of cancer tumors because it allows us to map the activity of genes within single tumor cells.”


Story Source:

The above story is based on materials provided by University of Zurich. Note: Materials may be edited for content and length.


Journal Reference:

  1. Nico Battich, Thomas Stoeger, Lucas Pelkmans. Image-based transcriptomics in thousands of single human cells at single-molecule resolution. Nature Methods, October 2013

Cite This Page:

University of Zurich. "First-ever view of gene activity, transcript patterns in single human cells." ScienceDaily. ScienceDaily, 6 October 2013. <www.sciencedaily.com/releases/2013/10/131006161357.htm>.
University of Zurich. (2013, October 6). First-ever view of gene activity, transcript patterns in single human cells. ScienceDaily. Retrieved September 22, 2014 from www.sciencedaily.com/releases/2013/10/131006161357.htm
University of Zurich. "First-ever view of gene activity, transcript patterns in single human cells." ScienceDaily. www.sciencedaily.com/releases/2013/10/131006161357.htm (accessed September 22, 2014).

Share This



More Plants & Animals News

Monday, September 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: San Diego Zoo Welcomes Cheetah Cubs

Raw: San Diego Zoo Welcomes Cheetah Cubs

AP (Sep. 20, 2014) The San Diego Zoo has welcomed two Cheetah cubs to its Safari Park. The nearly three-week-old female cubs are being hand fed and are receiving around the clock care. (Sept. 20) Video provided by AP
Powered by NewsLook.com
Chocolate Museum Opens in Brussels

Chocolate Museum Opens in Brussels

AFP (Sep. 19, 2014) Considered a "national heritage" in Belgium, chocolate now has a new museum in Brussels. In a former chocolate factory, visitors to the permanent exhibition spaces, workshops and tastings can discover derivatives of the cocoa bean. Duration: 01:00 Video provided by AFP
Powered by NewsLook.com
Could Grief Affect The Immune Systems Of Senior Citizens?

Could Grief Affect The Immune Systems Of Senior Citizens?

Newsy (Sep. 19, 2014) The study found elderly people are much more likely to become susceptible to infection than younger adults going though a similar situation. Video provided by Newsy
Powered by NewsLook.com
Jury Delivers Verdict in Salmonella Trial

Jury Delivers Verdict in Salmonella Trial

AP (Sep. 19, 2014) A federal jury has convicted three people in connection with an outbreak of salmonella poisoning five years ago that sickened hundreds of people and was linked to a number of deaths. (Sept. 19) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins