Featured Research

from universities, journals, and other organizations

Large-scale erythrocyte production using erythrocyte progenitor cells

Date:
December 5, 2013
Source:
Center for iPS Cell Research and Application - Kyoto University
Summary:
By transducing two genes (c-MYC and BCL-XL) into iPS cells and ES cells, a research team has succeeded in producing erythrocyte progenitor cells with almost unlimited ability to replicate in vitro, which they then differentiated successfully into mature erythrocytes. Although these erythrocytes consisted mostly of fetal-type hemoglobin, they were confirmed to have oxygen-carrying capacity and to have circulatory capacity following transfusion into mice.

By transducing two genes (c-MYC and BCL-XL) into iPS cells and ES cells, a Kyoto University research team has succeeded in producing erythrocyte progenitor cells with almost unlimited ability to replicate in vitro, which they then differentiated successfully into mature erythrocytes. Although these erythrocytes consisted mostly of fetal-type hemoglobin, they were confirmed to have oxygen-carrying capacity and to have circulatory capacity following transfusion into mice. Going forward, this technology is expected to contribute to a more reliable transfusion system by offering a new option that allows a stable supply of erythrocytes to be produced without depending on the availability of donor blood.

The findings of the research team, which included Sho-ichi Hirose (University of Tokyo researcher; currently TERUMO corporation), former Assistant Professor Naoya Takayama (Kyoto University, CiRA; currently researcher at Ontario Institute for Cancer Research), and Professor Koji Eto (Kyoto University, CiRA), are due to be published at 12:00 noon on December 5, 2013 (U.S Eastern time) in the online edition of the U.S. scientific journal Stem Cell Reports.

Erythrocytes, which have the function of transporting oxygen within the body, have no nucleus and are therefore not capable of self-replication. As a result, patients with blood diseases that cause serious anemia are currently dependent on the transfusion of blood products prepared from donated blood. Unfortunately, the number of blood donors is on the decline, mainly due to demographic changes. Hopes for the creation of a more stable transfusion system have centered on the establishment of a technology for large-scale erythrocyte production ex vivo, using for instance iPS cells or ES cells. Up to now, however, there have been no reports of erythrocyte production in sufficient quantities for use in transfusion.

Erythrocytes are produced in the body by progressive evolution of hematopoietic stem cells into hematopoietic progenitor cells and then erythroblasts. During the differentiation process from hematopoietic progenitor cells, chromosomes in the cell nucleus aggregate and the cell becomes a mature erythrocyte by subsequently losing this nucleus (enucleation). Hematopoietic progenitor cells, however, still have self-replication ability, and if the factors involved in this self-replication could be identified, it might become possible to induce virtually unlimited replication of progenitor cells. The research team had already succeeded in producing megakaryocytes (cells from which platelets develop) with almost unlimited replication ability, but in this latest research, aimed at creating erythrocyte progenitor cells which would have a similar almost unlimited replication ability, they transduced two genes (c-MYC, BCL-XL) into iPS cells and ES cells, which resulted in the production of erythrocyte progenitor cells with virtually unlimited in vitro replication ability.

The research showed that an increase in the expression of the c-MYC gene led to an enhancement of the replication ability of erythrocyte progenitor cells. It was also found that the expression of the BCL-XL gene was higher during the maturation process of erythrocyte progenitor cells than during their replication process. These findings indicate that these genes are important for the maturation of erythrocytes. As the c-MYC gene and BCL-XL gene are present within erythrocyte progenitor cells, methods that make use of them actually mimic the body's own system and can therefore be thought of as safer with a view to future clinical application than the previously developed systems for large-scale erythrocyte production using oncogene-derived from Human papillomavirus that are not normally expressed in the human body. According to rough calculations, the amount of culture medium needed to produce enough erythrocytes for a normal transfusion pack (containing one trillion units) is 1,000 -- 2,000 liters when using direct differentiation from undifferentiated iPS cells, whereas 50-100 liters is sufficient with the newly developed method. The same results were achieved with cells placed in frozen storage and then thawed. If a more efficient enucleation method can be established along with a method for generating erythrocytes containing only adult-type hemoglobin, progress will have been made toward a more reliable supply of erythrocytes for transfusion in the future.


Story Source:

The above story is based on materials provided by Center for iPS Cell Research and Application - Kyoto University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Sho-ichi Hirose, Naoya Takayama, Sou Nakamura, Kazumichi Nagasawa, Kiyosumi Ochi, Shinji Hirata, Satoshi Yamazaki, Tomoyuki Yamaguchi, Makoto Otsu, Shinya Sano, Nobuyasu Takahashi, Akira Sawaguchi, Mamoru Ito, Takashi Kato, Hiromitsu Nakauchi, Koji Eto. Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells. Stem Cell Reports, 2013; DOI: 10.1016/j.stemcr.2013.10.010

Cite This Page:

Center for iPS Cell Research and Application - Kyoto University. "Large-scale erythrocyte production using erythrocyte progenitor cells." ScienceDaily. ScienceDaily, 5 December 2013. <www.sciencedaily.com/releases/2013/12/131205141902.htm>.
Center for iPS Cell Research and Application - Kyoto University. (2013, December 5). Large-scale erythrocyte production using erythrocyte progenitor cells. ScienceDaily. Retrieved July 29, 2014 from www.sciencedaily.com/releases/2013/12/131205141902.htm
Center for iPS Cell Research and Application - Kyoto University. "Large-scale erythrocyte production using erythrocyte progenitor cells." ScienceDaily. www.sciencedaily.com/releases/2013/12/131205141902.htm (accessed July 29, 2014).

Share This




More Plants & Animals News

Tuesday, July 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deadly Ebola Virus Threatens West Africa

Deadly Ebola Virus Threatens West Africa

AP (July 28, 2014) West African nations and international health organizations are working to contain the largest Ebola outbreak in history. It's one of the deadliest diseases known to man, but the CDC says it's unlikely to spread in the U.S. (July 28) Video provided by AP
Powered by NewsLook.com
Traditional African Dishes Teach Healthy Eating

Traditional African Dishes Teach Healthy Eating

AP (July 28, 2014) Classes are being offered nationwide to encourage African Americans to learn about cooking fresh foods based on traditional African cuisine. The program is trying to combat obesity, heart disease and other ailments often linked to diet. (July 28) Video provided by AP
Powered by NewsLook.com
Asteroid's Timing Was 'Colossal Bad Luck' For The Dinosaurs

Asteroid's Timing Was 'Colossal Bad Luck' For The Dinosaurs

Newsy (July 28, 2014) The asteroid that killed the dinosaurs struck at the worst time for them. A new study says that if it hit earlier or later, they might've survived. Video provided by Newsy
Powered by NewsLook.com
Raw: Sea Turtle Hatchlings Emerge from Nest

Raw: Sea Turtle Hatchlings Emerge from Nest

AP (July 27, 2014) A live-streaming webcam catches loggerhead sea turtle hatchlings emerging from a nest in the Florida Keys. (July 27) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins