Featured Research

from universities, journals, and other organizations

Protocol developed to harvest mouse cell lines for melanoma research

Date:
January 31, 2014
Source:
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center
Summary:
Researchers have developed a protocol that permits cells harvested from melanoma tumors in mice to grow readily in cell culture.

Dartmouth researchers have developed a protocol that permits cells harvested from melanoma tumors in mice to grow readily in cell culture. Their findings were published in an article, Multiple murine BRafV600E melanoma cell lines with sensitivity to PLX4032, in the January 25, 2014 issue of Pigment Cell & Melanoma Research.

Related Articles


"We anticipate that these cell lines will be extremely useful to many investigators who use mouse melanoma as a model system," said Constance E. Brinckerhoff, PhD, professor of Medicine and of Biochemistry at the Geisel School of Medicine at Dartmouth College and a member of the Norris Cotton Cancer Center (NCCC) Mechanism Research Program.

There is a lack of mouse cell lines that harbor the BRAF mutation that is so prevalent in human melanomas, and the cell lines that are available grow slowly in culture and are not representative of human melanoma cell lines. Detailed experiments on molecular mechanisms controlling mouse cell line behavior have been difficult because the currently available mouse cell lines do not grow well in culture.

The Geisel researchers are the first to have developed a protocol that permits mouse melanoma cells to be harvested from tumors in the mice and to grow readily in cell culture. Importantly, these cell lines are genetically compatible with a strain of mice that are immunologically competent, while human cells need to be placed into immunologically weakened mice in order to grow. Thus, the ability to study these mouse melanoma cell lines both in culture and in mice with an intact immune system is an experimental advantage.


Story Source:

The above story is based on materials provided by Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. Note: Materials may be edited for content and length.


Journal Reference:

  1. Molly H. Jenkins, Shannon M. Steinberg, Matthew P. Alexander, Jan L. Fisher, Marc S. Ernstoff, Mary Jo Turk, David W. Mullins, Constance E. Brinckerhoff. Multiple murine BRafV600Emelanoma cell lines with sensitivity to PLX4032. Pigment Cell & Melanoma Research, 2014; DOI: 10.1111/pcmr.12220

Cite This Page:

Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. "Protocol developed to harvest mouse cell lines for melanoma research." ScienceDaily. ScienceDaily, 31 January 2014. <www.sciencedaily.com/releases/2014/01/140131130626.htm>.
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. (2014, January 31). Protocol developed to harvest mouse cell lines for melanoma research. ScienceDaily. Retrieved December 19, 2014 from www.sciencedaily.com/releases/2014/01/140131130626.htm
Norris Cotton Cancer CenterDartmouth-Hitchcock Medical Center. "Protocol developed to harvest mouse cell lines for melanoma research." ScienceDaily. www.sciencedaily.com/releases/2014/01/140131130626.htm (accessed December 19, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, December 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Navy Unveils Robot Fish

Navy Unveils Robot Fish

Reuters - Light News Video Online (Dec. 18, 2014) The U.S. Navy unveils an underwater device that mimics the movement of a fish. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
The Hottest Food Trends for 2015

The Hottest Food Trends for 2015

Buzz60 (Dec. 17, 2014) Urbanspoon predicts whicg food trends will dominate the culinary scene in 2015. Mara Montalbano (@maramontalbano) has the story. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins