Featured Research

from universities, journals, and other organizations

Faithful cell division requires tightly controlled protein placement at the centromeres

Date:
July 17, 2014
Source:
Whitehead Institute for Biomedical Research
Summary:
The protein CENP-A, which is integrated into human DNA at the centromere on each chromosome, has a vital role in cell division. New work describes how the vital and tightly controlled replenishment of CENP-A progresses. "The assumption was that the timing of CENP-A replenishment had to be important for centromere function because it was so tightly controlled. Now we have finally been able to test that assumption," says a co-author.

Images of cells with normal and abnormal CENP-A deposition. Centromere (red) location and proper division of chromosomes (blue) by microtubules (green) in mitosis require the protein CENP-A to be deposited in the correct place. When the the two kinases responsible for regulating this process--CDK and Plk1--are blocked, dividing cells have severe mitotic defects, including dramatically misaligned chromosomes and multipolar spindles (right), compared to normal cells (left).
Credit: Kara McKinley/Whitehead Institute

From fertilized egg to adult, the cells of the human body go through an astronomical number of divisions. During division of any of the body's roughly 30 trillion cells, DNA from the initial cell must be split precisely between the two resulting cells. Critical to successful cell division is the integrity of the centromere -- a region of DNA on each chromosome where the cell division machinery attaches to segregate the chromosomes. For the segregation machinery to recognize this region, it must contain many copies of a pivotal protein known as CENP-A.

Each time the cell makes a copy of its DNA in preparation for cell division, it needs to ensure that the centromeres of the new and old DNA strand get re-filled with CENP-A. Although scientists have known that the centromere replenishes its stock of CENP-A deposits during a phase of the cell cycle referred to as G1, exactly how this process is regulated had remained a mystery.

Now work by Whitehead Institute Member Iain Cheeseman and Kara McKinley, a graduate student in Cheeseman's lab has identified the molecular controls that ensure that CENP-A deposition at centromeres occurs in the right place at precisely the right time. Their findings are described in the current issue of the journal Cell.

In preparation for mitosis, the cell's DNA is copied and condensed into paired chromosomes. Thin protein fibers, called microtubules stretch out from points on opposite sides of the cell and latch onto a protein complex, called the kinetochore, which is anchored by CENP-A molecules at the centromere. As mitosis progresses, the microtubules align the chromosomes along the mid-line of the cell, then shorten and pull the chromosome pairs at their centromeres to opposite sides of the cell.

The fate of the cell depends on this process going smoothly. If the chromosomes have too many or too few centromeres, or the centromeres are located in the wrong place, proper chromosome segregation fails, and the cell either dies or becomes diseased. Because the centromere's integrity is so vital, its precise location is amazingly maintained each time cells divide, and passed on through multiple generations.

In her research, McKinley found that two kinases -- Plk1 and CDK -- work in separate steps to ensure that CENP-A replenishment proceeds correctly. For CENP-A to fill in all of the gaps in the centromere, both steps must work correctly. By defining the ways in which these kinases work, McKinley was able to disrupt the precise timing of CENP-A replenishment and cause severe problems for chromosome segregation.

"The assumption was that the timing of CENP-A replenishment had to be important for centromere function because it was so tightly controlled. Now we have finally been able to test that assumption," says McKinley.

"Kara's work has defined the basis for how the CENP-A deposition process is controlled," says Cheeseman, who is also associate professor of biology at Massachusetts Institute of Technology. "Since this step is such a central part of being a centromere, her work is a fundamental advance in how we think about this as a process."


Story Source:

The above story is based on materials provided by Whitehead Institute for Biomedical Research. The original article was written by Nicole Giese Rura. Note: Materials may be edited for content and length.


Journal Reference:

  1. KaraL. McKinley, IainM. Cheeseman. Polo-like Kinase 1 Licenses CENP-A Deposition at Centromeres. Cell, 2014; 158 (2): 397 DOI: 10.1016/j.cell.2014.06.016

Cite This Page:

Whitehead Institute for Biomedical Research. "Faithful cell division requires tightly controlled protein placement at the centromeres." ScienceDaily. ScienceDaily, 17 July 2014. <www.sciencedaily.com/releases/2014/07/140717124517.htm>.
Whitehead Institute for Biomedical Research. (2014, July 17). Faithful cell division requires tightly controlled protein placement at the centromeres. ScienceDaily. Retrieved September 16, 2014 from www.sciencedaily.com/releases/2014/07/140717124517.htm
Whitehead Institute for Biomedical Research. "Faithful cell division requires tightly controlled protein placement at the centromeres." ScienceDaily. www.sciencedaily.com/releases/2014/07/140717124517.htm (accessed September 16, 2014).

Share This



More Plants & Animals News

Tuesday, September 16, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Some Tobacco Farmers Thrive Amid Challenges

Some Tobacco Farmers Thrive Amid Challenges

AP (Sep. 16, 2014) The South's tobacco country is surviving, and even thriving in some cases, as demand overseas keeps growers in the fields of one of America's oldest cash crops. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Scientists Given Rare Glimpse of 350-Kilo Colossal Squid

Scientists Given Rare Glimpse of 350-Kilo Colossal Squid

AFP (Sep. 16, 2014) Scientists say a female colossal squid weighing an estimated 350 kilograms (770 lbs) and thought to be only the second intact specimen ever found was carrying eggs when discovered in the Antarctic. Duration: 00:47 Video provided by AFP
Powered by NewsLook.com
Raw: Scientists Examine Colossal Squid

Raw: Scientists Examine Colossal Squid

AP (Sep. 16, 2014) Squid experts in New Zealand thawed and examined an unusual catch on Tuesday: a colossal squid. It was captured in Antarctica's remote Ross Sea in December last year and has been frozen for eight months. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Conservationists Face Uphill PR Battle With New Shark Rules

Conservationists Face Uphill PR Battle With New Shark Rules

Newsy (Sep. 14, 2014) New conservation measures for shark fishing face an uphill PR battle in the fight to slow shark extinction. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

      Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins