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Strengthening legumes to tackle fertilizer pollution

April 23, 2013
DOE/Argonne National Laboratory
Scientists create the first model of legume iron transportation aimed at maximizing nitrogen fixation, even in poor soil.

Metal distribution in zone II of M. truncatula nodule.
Credit: Image courtesy of DOE/Argonne National Laboratory

The overuse of nitrogen fertilizers in agriculture can wreak havoc on waterways, health and the environment.

An international team of scientists aims to lessen the reliance on these fertilizers by helping beans and similar plants boost their nitrogen production, even in areas with traditionally poor soil quality.

Researchers from the Center of Plant Genomics and Biotechnology at the Technical University of Madrid (UPM) and the Advanced Photon Source (APS) at the U.S. Department of Energy's Argonne National Laboratory report as an advance article April 5 for the Metallomics journal of The Royal Society of Chemistry on how to use X-ray analysis to map a path to increasing the amount of nitrogen that legumes deposit into the soil.

Cultivation of legumes, the plant family that includes peas, beans, alfalfa, soybeans, and peanuts, is one of the main ways farmers add natural nitrogen to agricultural fields. Rotating bean and corn crops to take advantage of the nitrogen beans deposit in the soil has long been a global farming tradition. Legumes use iron in the soil to carry out a complex chemical process called nitrogen fixation, which collects atmospheric nitrogen and converts it into organic forms that help the plant grow. When the plant dies, the excess nitrogen is released back into to the soil to help the next crop.

But often legumes are grown in areas with iron-depleted soil, which limits their nitrogen fixation. That's where research can lend a hand. The Argonne-UPM team has created the world's first model for how iron is transported in the plant's root nodule to trigger nitrogen fixation. This is the first step in modifying the plants to maximize iron use.

"The long-term goal is to help sustainable agriculture practices and further diminish the environmental damage from overuse of nitrogen fertilizers," said Manuel Gonzalez-Guerrero, lead author of the paper from UPM. "This can be done by maximizing the delivery of essential metal oligonutrients to nitrogen-fixing rhizobia."

The research team, which included Lydia Finney and Stefan Vogt from the APS, used high-energy X-rays from the 8-BM and 2-ID-E beamlines of the APS to track the distribution of minute iron amounts in the different developmental regions of rhizobia-containing roots. This is the first high-energy X-ray analysis of plant-microbe interactions.

X-rays, such as those from the APS, provided a high sensitivity to elements and a high spatial resolution not attainable by other means. Full details can be found in the paper Iron distribution through the developmental stages of Medicago truncatula nodules.

In future studies at the APS, Gonzalez-Guerrero hopes to identify and characterize the key biological proteins responsible for iron transportation. That would give researchers targets to manipulate and screen for new legume varieties with increased nitrogen-fixation capabilities and higher nutritional value.

Story Source:

Materials provided by DOE/Argonne National Laboratory. Original written by Tona Kunz. Note: Content may be edited for style and length.

Journal Reference:

  1. Benjamín Rodríguez-Haas, Lydia Finney, Stefan Vogt, Pablo González-Melendi, Juan Imperial, Manuel Gonzalez-Guerrero. Iron distribution through the developmental stages of Medicago truncatula nodules. Metallomics, 2013; DOI: 10.1039/C3MT00060E

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DOE/Argonne National Laboratory. "Strengthening legumes to tackle fertilizer pollution." ScienceDaily. ScienceDaily, 23 April 2013. <>.
DOE/Argonne National Laboratory. (2013, April 23). Strengthening legumes to tackle fertilizer pollution. ScienceDaily. Retrieved April 28, 2017 from
DOE/Argonne National Laboratory. "Strengthening legumes to tackle fertilizer pollution." ScienceDaily. (accessed April 28, 2017).