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X-ray ptychography, fluorescence microscopy combo sheds new light on trace elements

Date:
April 13, 2015
Source:
DOE/Argonne National Laboratory
Summary:
A new approach that combines ptychographic X-ray imaging and fluorescence microscopy has been developed by scientists to study the important role trace elements play in biological functions on hydrated cells.
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Scientists have developed a new approach that combines ptychographic X-ray imaging and fluorescence microscopy to study the important role trace elements play in biological functions on hydrated cells.

A team of researchers using the Advanced Photon Source, a U.S. Department of Energy Office of Science User Facility at Argonne National Laboratory, demonstrated unparalleled sensitivity for measuring the distribution of trace elements in thicker specimens at cryogenic temperatures, in this case at about 260 degrees below Fahrenheit.

Trace metals are important in normal and disease-causing biological functions. Until now, researchers believe, it was impossible to obtain images of biological samples with such high combined structural and chemical fidelity.

"X-ray fluorescence lets us see trace metals in cells with fantastic sensitivity, and ptychography lets us correlate that with cellular ultrastructure," said Junjing Deng, an applied physics graduate student at Northwestern University. Ptychography is an approach to obtain absorption and phase contrast images from scattered light with no lens-imposed resolution limit, making use of the overlap between multiple coherent illumination spots.

Scientists studied a frozen-hydrated green algae specimen. Frozen hydrated samples under cryogenic conditions are better able to withstand beam-induced degradation and can provide high-fidelity structural and ionic elemental preservation.

"This advance required the high brightness of the APS as an X-ray source and points the way to advances that can be expected as it is planned to be increased a hundredfold in the future," said Chris Jacobsen, an associate division director in the X-ray Sciences Division at Argonne and a professor at Northwestern University.


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Materials provided by DOE/Argonne National Laboratory. Note: Content may be edited for style and length.


Journal Reference:

  1. Junjing Deng, David J. Vine, Si Chen, Youssef S. G. Nashed, Qiaoling Jin, Nicholas W. Phillips, Tom Peterka, Rob Ross, Stefan Vogt, Chris J. Jacobsen. Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae. Proceedings of the National Academy of Sciences, 2015; 112 (8): 2314 DOI: 10.1073/pnas.1413003112

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DOE/Argonne National Laboratory. "X-ray ptychography, fluorescence microscopy combo sheds new light on trace elements." ScienceDaily. ScienceDaily, 13 April 2015. <www.sciencedaily.com/releases/2015/04/150413130821.htm>.
DOE/Argonne National Laboratory. (2015, April 13). X-ray ptychography, fluorescence microscopy combo sheds new light on trace elements. ScienceDaily. Retrieved May 24, 2017 from www.sciencedaily.com/releases/2015/04/150413130821.htm
DOE/Argonne National Laboratory. "X-ray ptychography, fluorescence microscopy combo sheds new light on trace elements." ScienceDaily. www.sciencedaily.com/releases/2015/04/150413130821.htm (accessed May 24, 2017).

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