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Moonlighting molecules: Finding new uses for old enzymes

Date:
November 30, 2015
Source:
University of Cambridge
Summary:
Researchers have identified a potentially significant new application for a well-known human enzyme, which may have implications for treating respiratory diseases such as asthma.
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A collaboration between the University of Cambridge and MedImmune, the global biologics research and development arm of AstraZeneca, has led researchers to identify a potentially significant new application for a well-known human enzyme, which may have implications for treating respiratory diseases such as asthma.

Enzymes are biological catalysts -- molecules that speed up chemical reactions within living materials. Many enzymes are already well characterised and their functions fairly well understood. For example, the enzyme known as MMP8 is present in the connective tissue of most mammals, where it breaks the chemical bonds found in collagen.

In pre-clinical research published in the journal Chemistry & Biology, Dr Florian Hollfelder from the Department of Biochemistry at Cambridge and Dr Lutz Jermutus,Senior Director, Research and Development at MedImmune, led a study to map a list of human enzymes (proteases) against potential protein drug targets.

Using automation technology at MedImmune, the team then tested each of the enzymes against each target protein in turn, allowing them to identify a significant number of so-far unknown interactions.

Of particular interest was how MMP8 was able to disable a molecule known as IL-13, which is known to play an important role in several inflammatory diseases such as asthma and dermatitis. The researchers believe this may be a previously-unknown way in which the body regulates the action of IL-13, preventing these diseases in the majority of individuals. If so, it could provide an interesting target for new drugs against these common diseases.

"MMP8 is well-known to biochemists and we all thought we understood its function, but it's clear that this -- and probably many other enzymes -- 'moonlight' and have several functions within the body," explains Dr Hollfelder. "Because the enzyme already had a 'name' and a function, nobody thought to see if it had a promiscuous side."

Designing new enzymes has proven an extremely difficult technical challenge, hence the drive to find new uses for previously 'understood' enzymes. By focusing on human proteases, rather than bacterial proteases -- which are actually easier to source -- the researchers are confident that their research will be far more applicable to drug discovery.

"Our approach is new: we 'recycle' known enzymes and ask whether they can do other things than the ones they are known for," adds Dr Jermutus. "In fact, we believe we have found other enzymes that could be similarly deployed against other disease-causing proteins, and this approach, if expanded, could provide further leads for new drugs."

Commenting on the benefits of the collaboration with industry, Dr Hollfelder adds: "Without MedImmune, our work would have stopped after seeing and characterising the interactions. The additional extension to cell and mouse models would have been inconceivable in my basic science group."


Story Source:

Materials provided by University of Cambridge. The original story is licensed under a Creative Commons Licence. Note: Content may be edited for style and length.


Journal Reference:

  1. Carole Urbach, Nathaniel C. Gordon, Ian Strickland, David Lowne, Cathy Joberty-Candotti, Richard May, Athula Herath, DirkJan Hijnen, Judith L. Thijs, Carla A. Bruijnzeel-Koomen, Ralph R. Minter, Florian Hollfelder, Lutz Jermutus. Combinatorial Screening Identifies Novel Promiscuous Matrix Metalloproteinase Activities that Lead to Inhibition of the Therapeutic Target IL-13. Chemistry & Biology, 2015; 22 (11): 1442 DOI: 10.1016/j.chembiol.2015.09.013

Cite This Page:

University of Cambridge. "Moonlighting molecules: Finding new uses for old enzymes." ScienceDaily. ScienceDaily, 30 November 2015. <www.sciencedaily.com/releases/2015/11/151130111147.htm>.
University of Cambridge. (2015, November 30). Moonlighting molecules: Finding new uses for old enzymes. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2015/11/151130111147.htm
University of Cambridge. "Moonlighting molecules: Finding new uses for old enzymes." ScienceDaily. www.sciencedaily.com/releases/2015/11/151130111147.htm (accessed May 26, 2017).

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